Mouse models and human islet transplantation sites for intravital imaging
Human islet transplantations into rodent models are an essential tool to aid in the development and testing of islet and cellular-based therapies for diabetes prevention and treatment. Through the ability to evaluate human islets in an in vivo setting, these studies allow for experimental approaches...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.992540/full |
| _version_ | 1797997459303038976 |
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| author | Leslie E. Wagner Olha Melnyk Bryce E. Duffett Amelia K. Linnemann Amelia K. Linnemann Amelia K. Linnemann |
| author_facet | Leslie E. Wagner Olha Melnyk Bryce E. Duffett Amelia K. Linnemann Amelia K. Linnemann Amelia K. Linnemann |
| author_sort | Leslie E. Wagner |
| collection | DOAJ |
| description | Human islet transplantations into rodent models are an essential tool to aid in the development and testing of islet and cellular-based therapies for diabetes prevention and treatment. Through the ability to evaluate human islets in an in vivo setting, these studies allow for experimental approaches to answer questions surrounding normal and disease pathophysiology that cannot be answered using other in vitro and in vivo techniques alone. Intravital microscopy enables imaging of tissues in living organisms with dynamic temporal resolution and can be employed to measure biological processes in transplanted human islets revealing how experimental variables can influence engraftment, and transplant survival and function. A key consideration in experimental design for transplant imaging is the surgical placement site, which is guided by the presence of vasculature to aid in functional engraftment of the islets and promote their survival. Here, we review transplantation sites and mouse models used to study beta cell biology in vivo using intravital microscopy and we highlight fundamental observations made possible using this methodology. |
| first_indexed | 2024-04-11T10:32:13Z |
| format | Article |
| id | doaj.art-37c49744422749848259dae60fd1ed89 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-11T10:32:13Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-37c49744422749848259dae60fd1ed892022-12-22T04:29:23ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-10-011310.3389/fendo.2022.992540992540Mouse models and human islet transplantation sites for intravital imagingLeslie E. Wagner0Olha Melnyk1Bryce E. Duffett2Amelia K. Linnemann3Amelia K. Linnemann4Amelia K. Linnemann5Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, United StatesCenter for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, IN, United StatesHuman islet transplantations into rodent models are an essential tool to aid in the development and testing of islet and cellular-based therapies for diabetes prevention and treatment. Through the ability to evaluate human islets in an in vivo setting, these studies allow for experimental approaches to answer questions surrounding normal and disease pathophysiology that cannot be answered using other in vitro and in vivo techniques alone. Intravital microscopy enables imaging of tissues in living organisms with dynamic temporal resolution and can be employed to measure biological processes in transplanted human islets revealing how experimental variables can influence engraftment, and transplant survival and function. A key consideration in experimental design for transplant imaging is the surgical placement site, which is guided by the presence of vasculature to aid in functional engraftment of the islets and promote their survival. Here, we review transplantation sites and mouse models used to study beta cell biology in vivo using intravital microscopy and we highlight fundamental observations made possible using this methodology.https://www.frontiersin.org/articles/10.3389/fendo.2022.992540/fulldiabeteshuman isletsintravital microscopyislet transplantationhumanized mice |
| spellingShingle | Leslie E. Wagner Olha Melnyk Bryce E. Duffett Amelia K. Linnemann Amelia K. Linnemann Amelia K. Linnemann Mouse models and human islet transplantation sites for intravital imaging Frontiers in Endocrinology diabetes human islets intravital microscopy islet transplantation humanized mice |
| title | Mouse models and human islet transplantation sites for intravital imaging |
| title_full | Mouse models and human islet transplantation sites for intravital imaging |
| title_fullStr | Mouse models and human islet transplantation sites for intravital imaging |
| title_full_unstemmed | Mouse models and human islet transplantation sites for intravital imaging |
| title_short | Mouse models and human islet transplantation sites for intravital imaging |
| title_sort | mouse models and human islet transplantation sites for intravital imaging |
| topic | diabetes human islets intravital microscopy islet transplantation humanized mice |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.992540/full |
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