Photoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese Quail
The molecular mechanism underlying photoperiodic response in seasonal breeding animals such as the Japanese quail, red jungle fowl, sheep, mouse, and hamster involves thyroid-stimulating hormone beta subunit (TSHβ) mRNA expression in the pars tuberalis stimulated by the extension in day length. Furt...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2021-01-01
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| Series: | The Journal of Poultry Science |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/58/1/58_0190135/_html/-char/en |
| _version_ | 1827939058453577728 |
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| author | Nobuhiro Nakao Kei Nakagawa Asuna Sasaki Arisa Yamaguchi Nobumichi Tsushima Minoru Tanaka |
| author_facet | Nobuhiro Nakao Kei Nakagawa Asuna Sasaki Arisa Yamaguchi Nobumichi Tsushima Minoru Tanaka |
| author_sort | Nobuhiro Nakao |
| collection | DOAJ |
| description | The molecular mechanism underlying photoperiodic response in seasonal breeding animals such as the Japanese quail, red jungle fowl, sheep, mouse, and hamster involves thyroid-stimulating hormone beta subunit (TSHβ) mRNA expression in the pars tuberalis stimulated by the extension in day length. Furthermore, this mechanism is regulated by eyes absent 3 (Eya3) in mammals. Even in birds, the expression of both TSHβ and EYA3 is induced in the pars tuberalis by the extension in day length; however, the relationship between the two genes is unknown. To clarify the function of EYA3 in quail photoperiodism, in the present study, we performed mRNA structure analysis of the Japanese quail EYA3 mRNA using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. The results revealed that there are four types of splice variants within regions of exons 7, 8, and 9 of quail EYA3 mRNA. Among the four splice variants of quail EYA3, the splice variant containing exon 7 was expressed in the pars tuberalis on the first long day, when quails were transferred from the short-day condition to the long-day condition. The results indicate that EYA3 splice variant containing exon 7 is involved in the photoperiodic response of the pars tuberalis in the Japanese quail. |
| first_indexed | 2024-03-13T08:46:50Z |
| format | Article |
| id | doaj.art-37c4c085df0440e2ba75f8c850bb0a7a |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:50Z |
| publishDate | 2021-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-37c4c085df0440e2ba75f8c850bb0a7a2023-05-30T01:04:19ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862021-01-01581646910.2141/jpsa.0190135jpsaPhotoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese QuailNobuhiro Nakao0Kei Nakagawa1Asuna Sasaki2Arisa Yamaguchi3Nobumichi Tsushima4Minoru Tanaka5Department of Animal Science, Faculty of Applied Life Science, Nippon Veterinary and Life Science UniversityDepartment of Animal Science, Faculty of Applied Life Science, Nippon Veterinary and Life Science UniversityDepartment of Animal Science, Faculty of Applied Life Science, Nippon Veterinary and Life Science UniversityDepartment of Animal Science, Faculty of Applied Life Science, Nippon Veterinary and Life Science UniversityDepartment of Animal Science, Faculty of Applied Life Science, Nippon Veterinary and Life Science UniversityDepartment of Animal Science, Faculty of Applied Life Science, Nippon Veterinary and Life Science UniversityThe molecular mechanism underlying photoperiodic response in seasonal breeding animals such as the Japanese quail, red jungle fowl, sheep, mouse, and hamster involves thyroid-stimulating hormone beta subunit (TSHβ) mRNA expression in the pars tuberalis stimulated by the extension in day length. Furthermore, this mechanism is regulated by eyes absent 3 (Eya3) in mammals. Even in birds, the expression of both TSHβ and EYA3 is induced in the pars tuberalis by the extension in day length; however, the relationship between the two genes is unknown. To clarify the function of EYA3 in quail photoperiodism, in the present study, we performed mRNA structure analysis of the Japanese quail EYA3 mRNA using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. The results revealed that there are four types of splice variants within regions of exons 7, 8, and 9 of quail EYA3 mRNA. Among the four splice variants of quail EYA3, the splice variant containing exon 7 was expressed in the pars tuberalis on the first long day, when quails were transferred from the short-day condition to the long-day condition. The results indicate that EYA3 splice variant containing exon 7 is involved in the photoperiodic response of the pars tuberalis in the Japanese quail.https://www.jstage.jst.go.jp/article/jpsa/58/1/58_0190135/_html/-char/eneya3japanese quailphotoperiodismsplice variant |
| spellingShingle | Nobuhiro Nakao Kei Nakagawa Asuna Sasaki Arisa Yamaguchi Nobumichi Tsushima Minoru Tanaka Photoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese Quail The Journal of Poultry Science eya3 japanese quail photoperiodism splice variant |
| title | Photoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese Quail |
| title_full | Photoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese Quail |
| title_fullStr | Photoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese Quail |
| title_full_unstemmed | Photoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese Quail |
| title_short | Photoperiod-Specific Expression of Eyes Absent 3 Splice Variant in the Pars Tuberalis of the Japanese Quail |
| title_sort | photoperiod specific expression of eyes absent 3 splice variant in the pars tuberalis of the japanese quail |
| topic | eya3 japanese quail photoperiodism splice variant |
| url | https://www.jstage.jst.go.jp/article/jpsa/58/1/58_0190135/_html/-char/en |
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