Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties
Improvement of the medical textile industry has received more attention recently, especially with widespread of microbial and viral infections. Medical textiles with new properties, such as bacterial pathogens self-cleaning, have been explored with nanotechnology. In this study, an endophytic actino...
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MDPI AG
2020-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/10/641 |
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| author | Ahmed M. Eid Amr Fouda Gniewko Niedbała Saad El-Din Hassan Salem S. Salem Abdullah M. Abdo Helal F. Hetta Tharwat I. Shaheen |
| author_facet | Ahmed M. Eid Amr Fouda Gniewko Niedbała Saad El-Din Hassan Salem S. Salem Abdullah M. Abdo Helal F. Hetta Tharwat I. Shaheen |
| author_sort | Ahmed M. Eid |
| collection | DOAJ |
| description | Improvement of the medical textile industry has received more attention recently, especially with widespread of microbial and viral infections. Medical textiles with new properties, such as bacterial pathogens self-cleaning, have been explored with nanotechnology. In this study, an endophytic actinomycetes strain of <i>Streptomyces laurentii</i> R-1 was isolated from the roots of the medicinal plant <i>Achillea fragrantissima.</i> This is used as a catalyst for the mediated biosynthesis of silver nanoparticles (Ag-NPs) for applications in the textile industry. The biosynthesized Ag-NPs were characterized using UV-vis spectroscopy, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), and X-ray Diffraction (XRD), which confirmed the successful formation of crystalline, spherical metal nanoparticles. The biosynthesized Ag-NPs exhibited broad-spectrum antibacterial activity. Our data elucidated that the biosynthesized Ag-NPs had a highly cytotoxic effect against the cancerous caco-2 cell line. The selected safe dose of Ag-NPs for loading on cotton fabrics was 100 ppm, regarding their antibacterial activity and safe cytotoxic efficacy. Interestingly, scanning electron microscope connected with energy dispersive X-ray spectroscopy (SEM-EDX) of loaded cotton fabrics demonstrated the smooth distribution of Ag-NPs on treated fabrics. The obtained results highlighted the broad-spectrum activity of nano-finished fabrics against pathogenic bacteria, even after 5 and 10 washing cycles. This study contributes a suitable guide for the performance of green synthesized NPs for utilization in different biotechnological sectors. |
| first_indexed | 2024-03-10T16:04:32Z |
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| id | doaj.art-37c7df6d352947c6ad3c4d0b9355474f |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T16:04:32Z |
| publishDate | 2020-09-01 |
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| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-37c7df6d352947c6ad3c4d0b9355474f2023-11-20T15:00:43ZengMDPI AGAntibiotics2079-63822020-09-0191064110.3390/antibiotics9100641Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric PropertiesAhmed M. Eid0Amr Fouda1Gniewko Niedbała2Saad El-Din Hassan3Salem S. Salem4Abdullah M. Abdo5Helal F. Hetta6Tharwat I. Shaheen7Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Biosystems Engineering, Faculty of Environmental Engineering and Mechanical Engineering, Poznań University of Life Sciences, Wojska Polskiego 50, 60-627 Poznań, PolandDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, EgyptNational Research Centre, El-Behouth St., Dokki, P.O. Giza 12622, EgyptImprovement of the medical textile industry has received more attention recently, especially with widespread of microbial and viral infections. Medical textiles with new properties, such as bacterial pathogens self-cleaning, have been explored with nanotechnology. In this study, an endophytic actinomycetes strain of <i>Streptomyces laurentii</i> R-1 was isolated from the roots of the medicinal plant <i>Achillea fragrantissima.</i> This is used as a catalyst for the mediated biosynthesis of silver nanoparticles (Ag-NPs) for applications in the textile industry. The biosynthesized Ag-NPs were characterized using UV-vis spectroscopy, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), and X-ray Diffraction (XRD), which confirmed the successful formation of crystalline, spherical metal nanoparticles. The biosynthesized Ag-NPs exhibited broad-spectrum antibacterial activity. Our data elucidated that the biosynthesized Ag-NPs had a highly cytotoxic effect against the cancerous caco-2 cell line. The selected safe dose of Ag-NPs for loading on cotton fabrics was 100 ppm, regarding their antibacterial activity and safe cytotoxic efficacy. Interestingly, scanning electron microscope connected with energy dispersive X-ray spectroscopy (SEM-EDX) of loaded cotton fabrics demonstrated the smooth distribution of Ag-NPs on treated fabrics. The obtained results highlighted the broad-spectrum activity of nano-finished fabrics against pathogenic bacteria, even after 5 and 10 washing cycles. This study contributes a suitable guide for the performance of green synthesized NPs for utilization in different biotechnological sectors.https://www.mdpi.com/2079-6382/9/10/641silver nanoparticlesendophytic actinomycetesgreen synthesiscotton fabricsantibacterial |
| spellingShingle | Ahmed M. Eid Amr Fouda Gniewko Niedbała Saad El-Din Hassan Salem S. Salem Abdullah M. Abdo Helal F. Hetta Tharwat I. Shaheen Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties Antibiotics silver nanoparticles endophytic actinomycetes green synthesis cotton fabrics antibacterial |
| title | Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties |
| title_full | Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties |
| title_fullStr | Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties |
| title_full_unstemmed | Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties |
| title_short | Endophytic <i>Streptomyces laurentii</i> Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties |
| title_sort | endophytic i streptomyces laurentii i mediated green synthesis of ag nps with antibacterial and anticancer properties for developing functional textile fabric properties |
| topic | silver nanoparticles endophytic actinomycetes green synthesis cotton fabrics antibacterial |
| url | https://www.mdpi.com/2079-6382/9/10/641 |
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