Simultaneous two-photon imaging and wireless EEG recording in mice
Background: In vivo two-photon imaging is a reliable method with high spatial resolution that allows observation of individual neuron and dendritic activity longitudinally. Neurons in local brain regions can be influenced by global brain states such as levels of arousal and attention that change ove...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024019418 |
| _version_ | 1797259920328884224 |
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| author | Bowon Kim Weihua Ding Liuyue Yang Qian Chen Jianren Mao Guoping Feng Jee Hyun Choi Shiqian Shen |
| author_facet | Bowon Kim Weihua Ding Liuyue Yang Qian Chen Jianren Mao Guoping Feng Jee Hyun Choi Shiqian Shen |
| author_sort | Bowon Kim |
| collection | DOAJ |
| description | Background: In vivo two-photon imaging is a reliable method with high spatial resolution that allows observation of individual neuron and dendritic activity longitudinally. Neurons in local brain regions can be influenced by global brain states such as levels of arousal and attention that change over relatively short time scales, such as minutes. As such, the scientific rigor of investigating regional neuronal activities could be enhanced by considering the global brain state. New method: In order to assess the global brain state during in vivo two-photon imaging, CBRAIN (collective brain research platform aided by illuminating neural activity), a wireless EEG collecting and labeling device, was controlled by the same computer of two-photon microscope. In an experiment to explore neuronal responses to isoflurane anesthesia through two-photon imaging, we investigated whether the response of individual cells correlated with concurrent EEG changes induced by anesthesia. Results: In two-photon imaging, calcium activities of the excitatory neurons in the primary somatosensory cortex disappeared in about 30s after to the initiation of isoflurane anesthesia. The simultaneously recorded EEG showed various transitional activity for about 7 min from the initiation of anesthesia and continued with burst and suppression alternating pattern thereafter. As such, there was a dissociation between excitatory neuron activity of the primary somatosensory cortex and the global brain activity under anesthesia. Comparison with existing method(s): Existing methods to combine two-photon and EEG recording used wired EEG recording. In this study, wireless EEG was used in conjunction with two-photon imaging, facilitated by CBRAIN. More importantly, built-in algorithms of the CBRAIN can automatically detect brain state such as sleep. The codes used for EEG classification are easy to use, with no prior experience required. Conclusion: Simultaneous recording of wireless EEG and two-photon imaging provides a practical way to capture individual neuronal activities with respect to global brain state in an experimental set-up. |
| first_indexed | 2024-03-07T15:46:18Z |
| format | Article |
| id | doaj.art-37cf5eee55094d6684bb014065b4b442 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:17:05Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-37cf5eee55094d6684bb014065b4b4422024-03-17T07:55:06ZengElsevierHeliyon2405-84402024-03-01105e25910Simultaneous two-photon imaging and wireless EEG recording in miceBowon Kim0Weihua Ding1Liuyue Yang2Qian Chen3Jianren Mao4Guoping Feng5Jee Hyun Choi6Shiqian Shen7Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA; Center for Neuroscience, Korea Institute of Science and Technology, Seoul, Republic of KoreaCenter for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USACenter for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USAMcGovern Institute for Brain Research and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge MA, USA; Current address: Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, ChinaCenter for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USAMcGovern Institute for Brain Research and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge MA, USACenter for Neuroscience, Korea Institute of Science and Technology, Seoul, Republic of KoreaCenter for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA; Corresponding author. 149 13th Street, room 6224, Boston, MA 02129, USA.Background: In vivo two-photon imaging is a reliable method with high spatial resolution that allows observation of individual neuron and dendritic activity longitudinally. Neurons in local brain regions can be influenced by global brain states such as levels of arousal and attention that change over relatively short time scales, such as minutes. As such, the scientific rigor of investigating regional neuronal activities could be enhanced by considering the global brain state. New method: In order to assess the global brain state during in vivo two-photon imaging, CBRAIN (collective brain research platform aided by illuminating neural activity), a wireless EEG collecting and labeling device, was controlled by the same computer of two-photon microscope. In an experiment to explore neuronal responses to isoflurane anesthesia through two-photon imaging, we investigated whether the response of individual cells correlated with concurrent EEG changes induced by anesthesia. Results: In two-photon imaging, calcium activities of the excitatory neurons in the primary somatosensory cortex disappeared in about 30s after to the initiation of isoflurane anesthesia. The simultaneously recorded EEG showed various transitional activity for about 7 min from the initiation of anesthesia and continued with burst and suppression alternating pattern thereafter. As such, there was a dissociation between excitatory neuron activity of the primary somatosensory cortex and the global brain activity under anesthesia. Comparison with existing method(s): Existing methods to combine two-photon and EEG recording used wired EEG recording. In this study, wireless EEG was used in conjunction with two-photon imaging, facilitated by CBRAIN. More importantly, built-in algorithms of the CBRAIN can automatically detect brain state such as sleep. The codes used for EEG classification are easy to use, with no prior experience required. Conclusion: Simultaneous recording of wireless EEG and two-photon imaging provides a practical way to capture individual neuronal activities with respect to global brain state in an experimental set-up.http://www.sciencedirect.com/science/article/pii/S2405844024019418Two-photonElectroencephalogram (EEG)AnesthesiaIsofluraneGlobal brain stateMulti-dimensional brain activity |
| spellingShingle | Bowon Kim Weihua Ding Liuyue Yang Qian Chen Jianren Mao Guoping Feng Jee Hyun Choi Shiqian Shen Simultaneous two-photon imaging and wireless EEG recording in mice Heliyon Two-photon Electroencephalogram (EEG) Anesthesia Isoflurane Global brain state Multi-dimensional brain activity |
| title | Simultaneous two-photon imaging and wireless EEG recording in mice |
| title_full | Simultaneous two-photon imaging and wireless EEG recording in mice |
| title_fullStr | Simultaneous two-photon imaging and wireless EEG recording in mice |
| title_full_unstemmed | Simultaneous two-photon imaging and wireless EEG recording in mice |
| title_short | Simultaneous two-photon imaging and wireless EEG recording in mice |
| title_sort | simultaneous two photon imaging and wireless eeg recording in mice |
| topic | Two-photon Electroencephalogram (EEG) Anesthesia Isoflurane Global brain state Multi-dimensional brain activity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024019418 |
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