Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation Sequencing
Abstract Background The clinical treatment of patients suspected of pulmonary infections often rely on empirical antibiotics. However, preliminary diagnoses were based on clinical manifestations and conventional microbiological tests, which could later be proved wrong. In this case, we presented a p...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-03-01
|
| Series: | BMC Pulmonary Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12890-022-01894-3 |
| _version_ | 1828256820642185216 |
|---|---|
| author | Ping Wei Yang Gao Jing Zhang Jianlong Lin Huibin Liu Keqiang Chen Weikai Lin Xiaojia Wang Chune Wang Chao Liu |
| author_facet | Ping Wei Yang Gao Jing Zhang Jianlong Lin Huibin Liu Keqiang Chen Weikai Lin Xiaojia Wang Chune Wang Chao Liu |
| author_sort | Ping Wei |
| collection | DOAJ |
| description | Abstract Background The clinical treatment of patients suspected of pulmonary infections often rely on empirical antibiotics. However, preliminary diagnoses were based on clinical manifestations and conventional microbiological tests, which could later be proved wrong. In this case, we presented a patient whose initial diagnosis was lung abscess, but antibiotic treatments had no effect, and metagenomic Next-Generation Sequencing (mNGS) indicated presence of neoplasm. Case presentation A 62-year-old female was diagnosed with lung abscess at three different health facilities. However, mNGS of bronchoalveolar lavage fluid did not support pulmonary infections. Rather, the copy number variation analysis using host DNA sequences suggested neoplasm. Using H&E staining and immunohistochemistry of lung biopsy, the patient was eventually diagnosed with lung squamous cell carcinoma. Conclusions mNGS not only detects pathogens and helps diagnose infectious diseases, but also has potential in detecting neoplasm via host chromosomal copy number analysis. This might be beneficial for febrile patients with unknown or complex etiology, especially when infectious diseases were initially suspected but empirical antibiotic regimen failed. |
| first_indexed | 2024-04-13T02:33:48Z |
| format | Article |
| id | doaj.art-37d0d96b32144f979cea61f969c47c97 |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-04-13T02:33:48Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-37d0d96b32144f979cea61f969c47c972022-12-22T03:06:28ZengBMCBMC Pulmonary Medicine1471-24662022-03-012211710.1186/s12890-022-01894-3Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation SequencingPing Wei0Yang Gao1Jing Zhang2Jianlong Lin3Huibin Liu4Keqiang Chen5Weikai Lin6Xiaojia Wang7Chune Wang8Chao Liu9Fujian University of Traditional Chinese MedicineSchool of Biological Science and Medical Engineering, Beihang UniversityThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityHangzhou Matridx Biotechnology Co., LtdThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityHangzhou Matridx Biotechnology Co., LtdAbstract Background The clinical treatment of patients suspected of pulmonary infections often rely on empirical antibiotics. However, preliminary diagnoses were based on clinical manifestations and conventional microbiological tests, which could later be proved wrong. In this case, we presented a patient whose initial diagnosis was lung abscess, but antibiotic treatments had no effect, and metagenomic Next-Generation Sequencing (mNGS) indicated presence of neoplasm. Case presentation A 62-year-old female was diagnosed with lung abscess at three different health facilities. However, mNGS of bronchoalveolar lavage fluid did not support pulmonary infections. Rather, the copy number variation analysis using host DNA sequences suggested neoplasm. Using H&E staining and immunohistochemistry of lung biopsy, the patient was eventually diagnosed with lung squamous cell carcinoma. Conclusions mNGS not only detects pathogens and helps diagnose infectious diseases, but also has potential in detecting neoplasm via host chromosomal copy number analysis. This might be beneficial for febrile patients with unknown or complex etiology, especially when infectious diseases were initially suspected but empirical antibiotic regimen failed.https://doi.org/10.1186/s12890-022-01894-3Metagenomic Next-Generation SequencingLung abscessLung squamous cell carcinomaPulmonary infectionCopy number variation |
| spellingShingle | Ping Wei Yang Gao Jing Zhang Jianlong Lin Huibin Liu Keqiang Chen Weikai Lin Xiaojia Wang Chune Wang Chao Liu Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation Sequencing BMC Pulmonary Medicine Metagenomic Next-Generation Sequencing Lung abscess Lung squamous cell carcinoma Pulmonary infection Copy number variation |
| title | Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation Sequencing |
| title_full | Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation Sequencing |
| title_fullStr | Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation Sequencing |
| title_full_unstemmed | Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation Sequencing |
| title_short | Diagnosis of lung squamous cell carcinoma based on metagenomic Next-Generation Sequencing |
| title_sort | diagnosis of lung squamous cell carcinoma based on metagenomic next generation sequencing |
| topic | Metagenomic Next-Generation Sequencing Lung abscess Lung squamous cell carcinoma Pulmonary infection Copy number variation |
| url | https://doi.org/10.1186/s12890-022-01894-3 |
| work_keys_str_mv | AT pingwei diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT yanggao diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT jingzhang diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT jianlonglin diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT huibinliu diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT keqiangchen diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT weikailin diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT xiaojiawang diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT chunewang diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing AT chaoliu diagnosisoflungsquamouscellcarcinomabasedonmetagenomicnextgenerationsequencing |