Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry
Amygdalin is a natural cyanogenic compound that plants produce in the fight against insects and herbivores. Excessive amounts of amygdalin by animals and humans can potentially lead to fatal intoxication. However, studies confirm that amygdalin has antitumor properties, including the ability to inhi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/10/1459 |
| _version_ | 1797550509842759680 |
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| author | Maria Guć Sandra Rutecka Grzegorz Schroeder |
| author_facet | Maria Guć Sandra Rutecka Grzegorz Schroeder |
| author_sort | Maria Guć |
| collection | DOAJ |
| description | Amygdalin is a natural cyanogenic compound that plants produce in the fight against insects and herbivores. Excessive amounts of amygdalin by animals and humans can potentially lead to fatal intoxication. However, studies confirm that amygdalin has antitumor properties, including the ability to inhibit the proliferation of cancer cells and to induce their apoptosis. The analysis of amygdalin in various matrices is an important analytical problem today. The publication presents the methodology of direct determination of amygdalin in water, sewage, and biological materials using electrospray ionization mass spectrometry (ESI-MS) and a new analytical method using flowing atmospheric-pressure afterglow mass spectrometry (FAPA-MS). The methods of analyte pre-concentration using a magnetic, molecularly imprinted polymer (mag-MIP) and the influence of interferents on the recorded spectra were discussed. Analytical parameters in ESI-MS and FAPA-MS methods were established. The linearity range was 4.5 µg L<sup>−1</sup>–45 mg L<sup>−1</sup> in positive mode ESI-MS and FAPA-MS. The limit of detection (LOD) for ESI-MS was 0.101 ± 0.003 µg L<sup>−1</sup> and the limit of quantification (LOQ) was 0.303 ± 0.009 µg L<sup>−1</sup>. In FAPA-MS, the LOD was 0.050 ± 0.002 µg L<sup>−1</sup> and the LOQ was 0.150 ± 0.006 µg L<sup>−1</sup>. The content of amygdalin in various matrices was determined. |
| first_indexed | 2024-03-10T15:30:20Z |
| format | Article |
| id | doaj.art-37d42ea48cb94c2e9dc960c04552e4dc |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T15:30:20Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-37d42ea48cb94c2e9dc960c04552e4dc2023-11-20T17:40:24ZengMDPI AGBiomolecules2218-273X2020-10-011010145910.3390/biom10101459Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass SpectrometryMaria Guć0Sandra Rutecka1Grzegorz Schroeder2Faculty of Chemistry, Adam Mickiewicz University in Poznan, Uniwersytetu Poznańskiego 8, 61-614 Poznań, PolandFaculty of Chemistry, Adam Mickiewicz University in Poznan, Uniwersytetu Poznańskiego 8, 61-614 Poznań, PolandFaculty of Chemistry, Adam Mickiewicz University in Poznan, Uniwersytetu Poznańskiego 8, 61-614 Poznań, PolandAmygdalin is a natural cyanogenic compound that plants produce in the fight against insects and herbivores. Excessive amounts of amygdalin by animals and humans can potentially lead to fatal intoxication. However, studies confirm that amygdalin has antitumor properties, including the ability to inhibit the proliferation of cancer cells and to induce their apoptosis. The analysis of amygdalin in various matrices is an important analytical problem today. The publication presents the methodology of direct determination of amygdalin in water, sewage, and biological materials using electrospray ionization mass spectrometry (ESI-MS) and a new analytical method using flowing atmospheric-pressure afterglow mass spectrometry (FAPA-MS). The methods of analyte pre-concentration using a magnetic, molecularly imprinted polymer (mag-MIP) and the influence of interferents on the recorded spectra were discussed. Analytical parameters in ESI-MS and FAPA-MS methods were established. The linearity range was 4.5 µg L<sup>−1</sup>–45 mg L<sup>−1</sup> in positive mode ESI-MS and FAPA-MS. The limit of detection (LOD) for ESI-MS was 0.101 ± 0.003 µg L<sup>−1</sup> and the limit of quantification (LOQ) was 0.303 ± 0.009 µg L<sup>−1</sup>. In FAPA-MS, the LOD was 0.050 ± 0.002 µg L<sup>−1</sup> and the LOQ was 0.150 ± 0.006 µg L<sup>−1</sup>. The content of amygdalin in various matrices was determined.https://www.mdpi.com/2218-273X/10/10/1459amygdalinmag-MIPESI-MSFAPA-MS |
| spellingShingle | Maria Guć Sandra Rutecka Grzegorz Schroeder Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry Biomolecules amygdalin mag-MIP ESI-MS FAPA-MS |
| title | Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry |
| title_full | Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry |
| title_fullStr | Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry |
| title_full_unstemmed | Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry |
| title_short | Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry |
| title_sort | analysis of amygdalin in various matrices using electrospray ionization and flowing atmospheric pressure afterglow mass spectrometry |
| topic | amygdalin mag-MIP ESI-MS FAPA-MS |
| url | https://www.mdpi.com/2218-273X/10/10/1459 |
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