Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD
Non-alcoholic fatty liver disease (NAFLD) is a multifactorial condition characterized from hypertriglyceridemia and hepatic fat accumulation, in the absence of alcohol intake. NAFLD starts as steatosis (NAFL), and the continued injury relative to the toxic fat induces inflammation, steatohepatitis (...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/6/1133 |
| _version_ | 1797444018216370176 |
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| author | Maria Principia Scavo Nicoletta Depalo Federica Rizzi Livianna Carrieri Grazia Serino Isabella Franco Caterina Bonfiglio Pasqua Letizia Pesole Raffaele Cozzolongo Vito Gianuzzi Maria Lucia Curri Alberto Ruben Osella Gianluigi Giannelli |
| author_facet | Maria Principia Scavo Nicoletta Depalo Federica Rizzi Livianna Carrieri Grazia Serino Isabella Franco Caterina Bonfiglio Pasqua Letizia Pesole Raffaele Cozzolongo Vito Gianuzzi Maria Lucia Curri Alberto Ruben Osella Gianluigi Giannelli |
| author_sort | Maria Principia Scavo |
| collection | DOAJ |
| description | Non-alcoholic fatty liver disease (NAFLD) is a multifactorial condition characterized from hypertriglyceridemia and hepatic fat accumulation, in the absence of alcohol intake. NAFLD starts as steatosis (NAFL), and the continued injury relative to the toxic fat induces inflammation, steatohepatitis (NASH), and HCC. One of the factors determining liver degeneration during the evolution of NAFLD is a modification of Wnt/Frizzled (FZD) signaling. In particular, an inhibition of Wnt signaling and an overexpression of a specific FZD receptor protein, namely, the FZD7, have been observed in NAFLD. Actually, the prognosis and the follow-up of NAFLD is not easy, and the liver biopsy is the gold standard for an accurate detection of liver fibrosis. In this study, the modulation of the FZD7 expression levels in plasma-derived exosomes of NAFLD-affected patients, before and after specific lifestyle interventions, were experimentally evaluated by Western blotting analysis. The experimental data were analyzed by an accurate statistical study that indicated, in the exosomes derived from plasma of NAFLD patients with moderate or severe steatosis, an average expression level of FZD7 that was significantly higher than healthy subjects at baseline; conversely, the values were normalized after 90 days of specific lifestyle interventions. The overall results suggested that the FZD7 delivered by exosomes represents a good candidate as a new and effective biomarker for diagnosis and prognosis of NAFLD. |
| first_indexed | 2024-03-09T13:05:31Z |
| format | Article |
| id | doaj.art-37d479ae4e3c49038e91c5f59afb094f |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T13:05:31Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-37d479ae4e3c49038e91c5f59afb094f2023-11-30T21:48:22ZengMDPI AGNutrients2072-66432022-03-01146113310.3390/nu14061133Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLDMaria Principia Scavo0Nicoletta Depalo1Federica Rizzi2Livianna Carrieri3Grazia Serino4Isabella Franco5Caterina Bonfiglio6Pasqua Letizia Pesole7Raffaele Cozzolongo8Vito Gianuzzi9Maria Lucia Curri10Alberto Ruben Osella11Gianluigi Giannelli12Laboratory of Personalized Medicine, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyInstitute for Chemical-Physical Processes, Italian National Research Council (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, ItalyInstitute for Chemical-Physical Processes, Italian National Research Council (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, ItalyLaboratory of Personalized Medicine, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyMolecular Medicine, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyLaboratory of Epidemiology and Biostatistics, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyLaboratory of Epidemiology and Biostatistics, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyDepartment of Pathology, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyDepartment of Gastroenterology, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Castellana Grotte, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyDepartment of Gastroenterology, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Castellana Grotte, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyInstitute for Chemical-Physical Processes, Italian National Research Council (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, ItalyLaboratory of Epidemiology and Biostatistics, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, ItalyScientific Direction, National Institute of Gastroenterology IRCCS “S. de Bellis”, Research Hospital, Castellana Grotte, 70013 Bari, ItalyNon-alcoholic fatty liver disease (NAFLD) is a multifactorial condition characterized from hypertriglyceridemia and hepatic fat accumulation, in the absence of alcohol intake. NAFLD starts as steatosis (NAFL), and the continued injury relative to the toxic fat induces inflammation, steatohepatitis (NASH), and HCC. One of the factors determining liver degeneration during the evolution of NAFLD is a modification of Wnt/Frizzled (FZD) signaling. In particular, an inhibition of Wnt signaling and an overexpression of a specific FZD receptor protein, namely, the FZD7, have been observed in NAFLD. Actually, the prognosis and the follow-up of NAFLD is not easy, and the liver biopsy is the gold standard for an accurate detection of liver fibrosis. In this study, the modulation of the FZD7 expression levels in plasma-derived exosomes of NAFLD-affected patients, before and after specific lifestyle interventions, were experimentally evaluated by Western blotting analysis. The experimental data were analyzed by an accurate statistical study that indicated, in the exosomes derived from plasma of NAFLD patients with moderate or severe steatosis, an average expression level of FZD7 that was significantly higher than healthy subjects at baseline; conversely, the values were normalized after 90 days of specific lifestyle interventions. The overall results suggested that the FZD7 delivered by exosomes represents a good candidate as a new and effective biomarker for diagnosis and prognosis of NAFLD.https://www.mdpi.com/2072-6643/14/6/1133NAFLDplasma-derived exosomesFrizzled 7low glycemic index Mediterranean dietphysical activity |
| spellingShingle | Maria Principia Scavo Nicoletta Depalo Federica Rizzi Livianna Carrieri Grazia Serino Isabella Franco Caterina Bonfiglio Pasqua Letizia Pesole Raffaele Cozzolongo Vito Gianuzzi Maria Lucia Curri Alberto Ruben Osella Gianluigi Giannelli Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD Nutrients NAFLD plasma-derived exosomes Frizzled 7 low glycemic index Mediterranean diet physical activity |
| title | Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD |
| title_full | Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD |
| title_fullStr | Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD |
| title_full_unstemmed | Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD |
| title_short | Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD |
| title_sort | exosomal fzd 7 expression is modulated by different lifestyle interventions in patients with nafld |
| topic | NAFLD plasma-derived exosomes Frizzled 7 low glycemic index Mediterranean diet physical activity |
| url | https://www.mdpi.com/2072-6643/14/6/1133 |
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