The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats
Aim: To evaluate the possible protective effect of pomegranate extract (PE) on rats following renal ischemia-reperfusion (I/R) injury. Materials and Methods: Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 minutes without ischemia. I/R grou...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Urology Annals |
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| Online Access: | http://www.urologyannals.com/article.asp?issn=0974-7796;year=2014;volume=6;issue=1;spage=46;epage=50;aulast=Sancaktutar |
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| author | Ahmet A Sancaktutar Mehmet N Bodakci Namık K Hatipoglu Haluk Soylemez Kemal Basarılı Gul Turkcu |
| author_facet | Ahmet A Sancaktutar Mehmet N Bodakci Namık K Hatipoglu Haluk Soylemez Kemal Basarılı Gul Turkcu |
| author_sort | Ahmet A Sancaktutar |
| collection | DOAJ |
| description | Aim: To evaluate the possible protective effect of pomegranate extract (PE) on rats following renal ischemia-reperfusion (I/R) injury.
Materials and Methods: Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 minutes without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 minutes of reperfusion. I/R + PE group were subjected to the same renal I/R as the I/R group were also given 225 mg/kg PE peroral 30 minutes prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues.
Results: Serum TAC levels were significantly decreased in I/R group when compared with S group (P = 0.001). Serum MDA levels were increased in I/R group; however, it was not statistically significant. In rat kidney tissues, TOS levels and OSI index were significantly increased after I/R injury, while TAC levels were decreased. In I/R + PE group, PE reversed the negative effects of I/R injury. PE pretreatment was effective in decreasing tubular necrosis score.
Conclusion: PE pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury. |
| first_indexed | 2024-12-10T12:45:17Z |
| format | Article |
| id | doaj.art-37d55ad0d533440d964c0e12553086e3 |
| institution | Directory Open Access Journal |
| issn | 0974-7796 0974-7834 |
| language | English |
| last_indexed | 2024-12-10T12:45:17Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Urology Annals |
| spelling | doaj.art-37d55ad0d533440d964c0e12553086e32022-12-22T01:48:25ZengWolters Kluwer Medknow PublicationsUrology Annals0974-77960974-78342014-01-0161465010.4103/0974-7796.127029The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male ratsAhmet A SancaktutarMehmet N BodakciNamık K HatipogluHaluk SoylemezKemal BasarılıGul TurkcuAim: To evaluate the possible protective effect of pomegranate extract (PE) on rats following renal ischemia-reperfusion (I/R) injury. Materials and Methods: Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 minutes without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 minutes of reperfusion. I/R + PE group were subjected to the same renal I/R as the I/R group were also given 225 mg/kg PE peroral 30 minutes prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues. Results: Serum TAC levels were significantly decreased in I/R group when compared with S group (P = 0.001). Serum MDA levels were increased in I/R group; however, it was not statistically significant. In rat kidney tissues, TOS levels and OSI index were significantly increased after I/R injury, while TAC levels were decreased. In I/R + PE group, PE reversed the negative effects of I/R injury. PE pretreatment was effective in decreasing tubular necrosis score. Conclusion: PE pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.http://www.urologyannals.com/article.asp?issn=0974-7796;year=2014;volume=6;issue=1;spage=46;epage=50;aulast=SancaktutarAntioxidantischemia-reperfusionkidneypomegranate extractrat |
| spellingShingle | Ahmet A Sancaktutar Mehmet N Bodakci Namık K Hatipoglu Haluk Soylemez Kemal Basarılı Gul Turkcu The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats Urology Annals Antioxidant ischemia-reperfusion kidney pomegranate extract rat |
| title | The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats |
| title_full | The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats |
| title_fullStr | The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats |
| title_full_unstemmed | The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats |
| title_short | The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats |
| title_sort | protective effects of pomegranate extracts against renal ischemia reperfusion injury in male rats |
| topic | Antioxidant ischemia-reperfusion kidney pomegranate extract rat |
| url | http://www.urologyannals.com/article.asp?issn=0974-7796;year=2014;volume=6;issue=1;spage=46;epage=50;aulast=Sancaktutar |
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