Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for to...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
|
| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S135041772300247X |
| _version_ | 1797755799049601024 |
|---|---|
| author | Josipa Dukić Karla Košpić Vanja Kelava Renata Mavrić Marinela Nutrizio Biljana Balen Ana Butorac Mecit Halil Öztop Anet Režek Jambrak |
| author_facet | Josipa Dukić Karla Košpić Vanja Kelava Renata Mavrić Marinela Nutrizio Biljana Balen Ana Butorac Mecit Halil Öztop Anet Režek Jambrak |
| author_sort | Josipa Dukić |
| collection | DOAJ |
| description | Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/gd.m. with cold and 96.75 ± 4.30 mg/gd.m. with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/gd.m. with argon and 34.89 ± 1.59 mg/gd.m. with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products. |
| first_indexed | 2024-03-12T17:51:21Z |
| format | Article |
| id | doaj.art-37d91030931c4c68a7453ce763f8c6cf |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-03-12T17:51:21Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-37d91030931c4c68a7453ce763f8c6cf2023-08-03T04:22:59ZengElsevierUltrasonics Sonochemistry1350-41772023-10-0199106535Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical dischargeJosipa Dukić0Karla Košpić1Vanja Kelava2Renata Mavrić3Marinela Nutrizio4Biljana Balen5Ana Butorac6Mecit Halil Öztop7Anet Režek Jambrak8Faculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, Croatia; Corresponding authors.Department of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia; Corresponding authors.BICRO BIOCentre Ltd, Cent Lab, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, CroatiaBICRO BIOCentre Ltd, Cent Lab, 10000 Zagreb, CroatiaDepartment of Food Engineering, Middle East Technical University, 06800 Ankara, TurkeyFaculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, CroatiaUltrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/gd.m. with cold and 96.75 ± 4.30 mg/gd.m. with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/gd.m. with argon and 34.89 ± 1.59 mg/gd.m. with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products.http://www.sciencedirect.com/science/article/pii/S135041772300247XZero waste conceptUltrasoundHigh voltage electrical dischargeSugar beet leavesProtein extractionRuBisCO |
| spellingShingle | Josipa Dukić Karla Košpić Vanja Kelava Renata Mavrić Marinela Nutrizio Biljana Balen Ana Butorac Mecit Halil Öztop Anet Režek Jambrak Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge Ultrasonics Sonochemistry Zero waste concept Ultrasound High voltage electrical discharge Sugar beet leaves Protein extraction RuBisCO |
| title | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
| title_full | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
| title_fullStr | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
| title_full_unstemmed | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
| title_short | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
| title_sort | alternative methods for rubisco extraction from sugar beet waste a comparative approach of ultrasound and high voltage electrical discharge |
| topic | Zero waste concept Ultrasound High voltage electrical discharge Sugar beet leaves Protein extraction RuBisCO |
| url | http://www.sciencedirect.com/science/article/pii/S135041772300247X |
| work_keys_str_mv | AT josipadukic alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT karlakospic alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT vanjakelava alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT renatamavric alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT marinelanutrizio alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT biljanabalen alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT anabutorac alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT mecithaliloztop alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge AT anetrezekjambrak alternativemethodsforrubiscoextractionfromsugarbeetwasteacomparativeapproachofultrasoundandhighvoltageelectricaldischarge |