Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq
klebsiella is the most common pathogen responsible for acquired infections in the hospitals and because of the extensive ability to produce β-lactamases made this bacteria resistance to many antimicrobial agents. This study was done to determine Klebsiella pneumoniae isolates containing both blaFOX...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2016-12-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4353 |
| _version_ | 1827389131736481792 |
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| author | Abeer Mohammed Ali Al-garawyi |
| author_facet | Abeer Mohammed Ali Al-garawyi |
| author_sort | Abeer Mohammed Ali Al-garawyi |
| collection | DOAJ |
| description | klebsiella is the most common pathogen responsible for acquired infections in the hospitals and because of the extensive ability to produce β-lactamases made this bacteria resistance to many antimicrobial agents. This study was done to determine Klebsiella pneumoniae isolates containing both blaFOX and blaCIT by multiplex polymerase chain reaction . Out of fifty one Klebsiella isolates , 31 (60.8%) K. pneumoniae isolates were resistant to cefoxitin by disk diffusion method . The production of molecular class C β-lactamase tested through modified three dimension method in all cefox resistance isolates and observed 14 (45.2%) isolates were producing for AmpC β-lactamase . The cefoxitin resistance isolates further screened by AmpC disk method and the result was 11 (35.5%)isolates were producing for AmpC β-lactamase . Multiplex PCR technique showed 8/31 (25.8%) K. pneumoniae isolates possess AmpC β- lactamase gene as well as this technique also revealed that FOX gene was exists in (37.5%) and CIT gene (62.5%)of the isolates . |
| first_indexed | 2024-03-08T16:29:05Z |
| format | Article |
| id | doaj.art-37db62e15e6240ee96894717e9927c75 |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:29:05Z |
| publishDate | 2016-12-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-37db62e15e6240ee96894717e9927c752024-01-06T10:17:21ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782016-12-017210.36326/kjvs/2016/v7i24353Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- IraqAbeer Mohammed Ali Al-garawyiklebsiella is the most common pathogen responsible for acquired infections in the hospitals and because of the extensive ability to produce β-lactamases made this bacteria resistance to many antimicrobial agents. This study was done to determine Klebsiella pneumoniae isolates containing both blaFOX and blaCIT by multiplex polymerase chain reaction . Out of fifty one Klebsiella isolates , 31 (60.8%) K. pneumoniae isolates were resistant to cefoxitin by disk diffusion method . The production of molecular class C β-lactamase tested through modified three dimension method in all cefox resistance isolates and observed 14 (45.2%) isolates were producing for AmpC β-lactamase . The cefoxitin resistance isolates further screened by AmpC disk method and the result was 11 (35.5%)isolates were producing for AmpC β-lactamase . Multiplex PCR technique showed 8/31 (25.8%) K. pneumoniae isolates possess AmpC β- lactamase gene as well as this technique also revealed that FOX gene was exists in (37.5%) and CIT gene (62.5%)of the isolates .https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4353AmpCCITFOX and PCRKlebsiella pneumoniae |
| spellingShingle | Abeer Mohammed Ali Al-garawyi Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq Kufa Journal for Veterinary Medical Sciences AmpC CIT FOX and PCR Klebsiella pneumoniae |
| title | Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq |
| title_full | Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq |
| title_fullStr | Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq |
| title_full_unstemmed | Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq |
| title_short | Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in Al-Muthanna Province- Iraq |
| title_sort | molecular expression of blacit and blafox in klebsiella spp isolates from al hussein teaching hospital in al muthanna province iraq |
| topic | AmpC CIT FOX and PCR Klebsiella pneumoniae |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4353 |
| work_keys_str_mv | AT abeermohammedalialgarawyi molecularexpressionofblacitandblafoxinklebsiellasppisolatesfromalhusseinteachinghospitalinalmuthannaprovinceiraq |