Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization
Abstract Fucoidan has attracted considerable attention from scientists and pharmaceutical companies due to its antioxidant, anticoagulant, anti-inflammatory, anti-tumor, and health-enhancing properties. However, the extraction of fucoidan from seaweeds often involves the use of harsh chemicals, whic...
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-55225-z |
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| author | Viruja Ummat Saravana Periaswamy Sivagnanam Dilip K. Rai Colm O’Donnell Gillian E. Conway Shane M. Heffernan Stephen Fitzpatrick Henry Lyons James Curtin Brijesh Kumar Tiwari |
| author_facet | Viruja Ummat Saravana Periaswamy Sivagnanam Dilip K. Rai Colm O’Donnell Gillian E. Conway Shane M. Heffernan Stephen Fitzpatrick Henry Lyons James Curtin Brijesh Kumar Tiwari |
| author_sort | Viruja Ummat |
| collection | DOAJ |
| description | Abstract Fucoidan has attracted considerable attention from scientists and pharmaceutical companies due to its antioxidant, anticoagulant, anti-inflammatory, anti-tumor, and health-enhancing properties. However, the extraction of fucoidan from seaweeds often involves the use of harsh chemicals, which necessitates the search for alternative solvents. Additionally, the high viscosity and low cell permeability of high molecular weight (Mw) fucoidan can limit its effectiveness in drug action, while lower Mw fractions exhibit increased biological activity and are also utilized as dietary supplements. The study aimed to (1) extract fucoidan from the seaweed Fucus vesiculosus (FV) using an environmentally friendly solvent and compare it with the most commonly used extraction solvent, hydrochloric acid, and (2) assess the impact of ultrasound-assisted depolymerization on reducing the molecular weight of the fucoidan extracts and examine the cytotoxic effect of different molecular weight fractions. The findings indicated that the green depolymerization solvent, in conjunction with a brief ultrasound treatment, effectively reduced the molecular weight. Moreover, a significant decrease in cell viability was observed in selected samples, indicating potential anticancer properties. As a result, ultrasound was determined to be an effective method for depolymerizing crude fucoidan from Fucus Vesiculosus seaweed. |
| first_indexed | 2024-04-24T23:07:53Z |
| format | Article |
| id | doaj.art-37dbdf482fb8455ba73061a9df2b217f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:07:53Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-37dbdf482fb8455ba73061a9df2b217f2024-03-17T12:23:29ZengNature PortfolioScientific Reports2045-23222024-03-0114111310.1038/s41598-024-55225-zConventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerizationViruja Ummat0Saravana Periaswamy Sivagnanam1Dilip K. Rai2Colm O’Donnell3Gillian E. Conway4Shane M. Heffernan5Stephen Fitzpatrick6Henry Lyons7James Curtin8Brijesh Kumar Tiwari9Teagasc Ashtown Food Research Centre, TeagascDepartment of Biological Sciences, Munster Technological UniversityTeagasc Ashtown Food Research Centre, TeagascUCD School of Biosystems and Food Engineering, University College DublinIn Vitro Toxicology Group, Institute of Life Science, College of Medicine, Swansea UniversityApplied Sports Science Technology and Medicine Research Centre (A-STEM), Faculty of Science and Engineering, Swansea UniversityNutramara Ltd.Nutramara Ltd.School of Food Science and Environmental Health, College of Science and Health, Technological University DublinTeagasc Ashtown Food Research Centre, TeagascAbstract Fucoidan has attracted considerable attention from scientists and pharmaceutical companies due to its antioxidant, anticoagulant, anti-inflammatory, anti-tumor, and health-enhancing properties. However, the extraction of fucoidan from seaweeds often involves the use of harsh chemicals, which necessitates the search for alternative solvents. Additionally, the high viscosity and low cell permeability of high molecular weight (Mw) fucoidan can limit its effectiveness in drug action, while lower Mw fractions exhibit increased biological activity and are also utilized as dietary supplements. The study aimed to (1) extract fucoidan from the seaweed Fucus vesiculosus (FV) using an environmentally friendly solvent and compare it with the most commonly used extraction solvent, hydrochloric acid, and (2) assess the impact of ultrasound-assisted depolymerization on reducing the molecular weight of the fucoidan extracts and examine the cytotoxic effect of different molecular weight fractions. The findings indicated that the green depolymerization solvent, in conjunction with a brief ultrasound treatment, effectively reduced the molecular weight. Moreover, a significant decrease in cell viability was observed in selected samples, indicating potential anticancer properties. As a result, ultrasound was determined to be an effective method for depolymerizing crude fucoidan from Fucus Vesiculosus seaweed.https://doi.org/10.1038/s41598-024-55225-zSeaweedFucoidanExtractionUltrasoundDepolymerization |
| spellingShingle | Viruja Ummat Saravana Periaswamy Sivagnanam Dilip K. Rai Colm O’Donnell Gillian E. Conway Shane M. Heffernan Stephen Fitzpatrick Henry Lyons James Curtin Brijesh Kumar Tiwari Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization Scientific Reports Seaweed Fucoidan Extraction Ultrasound Depolymerization |
| title | Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization |
| title_full | Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization |
| title_fullStr | Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization |
| title_full_unstemmed | Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization |
| title_short | Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization |
| title_sort | conventional extraction of fucoidan from irish brown seaweed fucus vesiculosus followed by ultrasound assisted depolymerization |
| topic | Seaweed Fucoidan Extraction Ultrasound Depolymerization |
| url | https://doi.org/10.1038/s41598-024-55225-z |
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