Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants
Abstract Background Extensive population growth and climate change accelerate the search for alternative ways of plant-based biomass, biofuel and feed production. Here, we focus on hitherto unknow, new promising cold-stimulated function of phospholipid:diacylglycerol acyltransferase1 (PDAT1) – an en...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04379-5 |
| _version_ | 1797769558759571456 |
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| author | Sylwia Klińska-Bąchor Sara Kędzierska Kamil Demski Antoni Banaś |
| author_facet | Sylwia Klińska-Bąchor Sara Kędzierska Kamil Demski Antoni Banaś |
| author_sort | Sylwia Klińska-Bąchor |
| collection | DOAJ |
| description | Abstract Background Extensive population growth and climate change accelerate the search for alternative ways of plant-based biomass, biofuel and feed production. Here, we focus on hitherto unknow, new promising cold-stimulated function of phospholipid:diacylglycerol acyltransferase1 (PDAT1) – an enzyme catalyzing the last step of triacylglycerol (TAG) biosynthesis. Result Overexpression of AtPDAT1 boosted seed yield by 160% in Arabidopsis plants exposed to long-term cold compared to standard conditions. Such seeds increased both their weight and acyl-lipids content. This work also elucidates PDAT1’s role in leaves, which was previously unclear. Aerial parts of AtPDAT1-overexpressing plants were characterized by accelerated growth at early and vegetative stages of development and by biomass weighing three times more than control. Overexpression of PDAT1 increased the expression of SUGAR-DEPENDENT1 (SDP1) TAG lipase and enhanced lipid remodeling, driving lipid turnover and influencing biomass increment. This effect was especially pronounced in cold conditions, where the elevated synergistic expression of PDAT1 and SDP1 resulted in double biomass increase compared to standard conditions. Elevated phospholipid remodeling also enhanced autophagy flux in AtPDAT1-overexpresing lines subjected to cold, despite the overall diminished autophagy intensity in cold conditions. Conclusions Our data suggest that PDAT1 promotes greater vitality in cold-exposed plants, stimulates their longevity and boosts oilseed oil production at low temperature. |
| first_indexed | 2024-03-12T21:10:48Z |
| format | Article |
| id | doaj.art-37dc4d6117ff4d4d9e60aa61f01f6fec |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-12T21:10:48Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-37dc4d6117ff4d4d9e60aa61f01f6fec2023-07-30T11:11:14ZengBMCBMC Plant Biology1471-22292023-07-0123112010.1186/s12870-023-04379-5Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plantsSylwia Klińska-Bąchor0Sara Kędzierska1Kamil Demski2Antoni Banaś3Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of GdańskIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of GdańskDepartment of Plant Breeding, Swedish University of Agricultural SciencesIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of GdańskAbstract Background Extensive population growth and climate change accelerate the search for alternative ways of plant-based biomass, biofuel and feed production. Here, we focus on hitherto unknow, new promising cold-stimulated function of phospholipid:diacylglycerol acyltransferase1 (PDAT1) – an enzyme catalyzing the last step of triacylglycerol (TAG) biosynthesis. Result Overexpression of AtPDAT1 boosted seed yield by 160% in Arabidopsis plants exposed to long-term cold compared to standard conditions. Such seeds increased both their weight and acyl-lipids content. This work also elucidates PDAT1’s role in leaves, which was previously unclear. Aerial parts of AtPDAT1-overexpressing plants were characterized by accelerated growth at early and vegetative stages of development and by biomass weighing three times more than control. Overexpression of PDAT1 increased the expression of SUGAR-DEPENDENT1 (SDP1) TAG lipase and enhanced lipid remodeling, driving lipid turnover and influencing biomass increment. This effect was especially pronounced in cold conditions, where the elevated synergistic expression of PDAT1 and SDP1 resulted in double biomass increase compared to standard conditions. Elevated phospholipid remodeling also enhanced autophagy flux in AtPDAT1-overexpresing lines subjected to cold, despite the overall diminished autophagy intensity in cold conditions. Conclusions Our data suggest that PDAT1 promotes greater vitality in cold-exposed plants, stimulates their longevity and boosts oilseed oil production at low temperature.https://doi.org/10.1186/s12870-023-04379-5Phospholipid:diacylglycerol acyltransferaseSeed yieldLipid remodelingOilseed biotechnologyLipid compositionAbiotic stress |
| spellingShingle | Sylwia Klińska-Bąchor Sara Kędzierska Kamil Demski Antoni Banaś Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants BMC Plant Biology Phospholipid:diacylglycerol acyltransferase Seed yield Lipid remodeling Oilseed biotechnology Lipid composition Abiotic stress |
| title | Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants |
| title_full | Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants |
| title_fullStr | Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants |
| title_full_unstemmed | Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants |
| title_short | Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants |
| title_sort | phospholipid diacylglycerol acyltransferase1 overexpression stimulates lipid turnover oil production and fitness in cold grown plants |
| topic | Phospholipid:diacylglycerol acyltransferase Seed yield Lipid remodeling Oilseed biotechnology Lipid composition Abiotic stress |
| url | https://doi.org/10.1186/s12870-023-04379-5 |
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