Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses
Abstract Background Elevated levels of environmental ionizing radiation can be a selective pressure for wildlife by producing reactive oxygen species and DNA damage. However, the underlying molecular mechanisms that are affected are not known. Results We isolated skin fibroblasts from bank voles (My...
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | BMC Cell Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12860-018-0169-9 |
| _version_ | 1818856893935779840 |
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| author | Venla Mustonen Jenni Kesäniemi Anton Lavrinienko Eugene Tukalenko Tapio Mappes Phillip C. Watts Jaana Jurvansuu |
| author_facet | Venla Mustonen Jenni Kesäniemi Anton Lavrinienko Eugene Tukalenko Tapio Mappes Phillip C. Watts Jaana Jurvansuu |
| author_sort | Venla Mustonen |
| collection | DOAJ |
| description | Abstract Background Elevated levels of environmental ionizing radiation can be a selective pressure for wildlife by producing reactive oxygen species and DNA damage. However, the underlying molecular mechanisms that are affected are not known. Results We isolated skin fibroblasts from bank voles (Myodes glareolus) inhabiting the Chernobyl nuclear power plant accident site where background radiation levels are about 100 times greater than in uncontaminated areas. After a 10 Gy dose of gamma radiation fibroblasts from Chernobyl animals recovered faster than fibroblasts isolated from bank voles living in uncontaminated control area. The Chernobyl fibroblasts were able to sustain significantly higher doses of an oxidant and they had, on average, a higher total antioxidant capacity than the control fibroblasts. Furthermore, the Chernobyl fibroblasts were also significantly more resistant than the control fibroblasts to continuous exposure to three DNA damaging drugs. After drug treatment transcription of p53-target gene pro-apoptotic Bax was higher in the control than in the Chernobyl fibroblasts. Conclusion Fibroblasts isolated from bank voles inhabiting Chernobyl nuclear power plant accident site show elevated antioxidant levels, lower sensitivity to apoptosis, and increased resistance against oxidative and DNA stresses. These cellular qualities may help bank voles inhabiting Chernobyl to cope with environmental radioactivity. |
| first_indexed | 2024-12-19T08:31:44Z |
| format | Article |
| id | doaj.art-37dce54af13f41beb8415484ededae60 |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-12-19T08:31:44Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
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| series | BMC Cell Biology |
| spelling | doaj.art-37dce54af13f41beb8415484ededae602022-12-21T20:29:10ZengBMCBMC Cell Biology1471-21212018-08-0119111010.1186/s12860-018-0169-9Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stressesVenla Mustonen0Jenni Kesäniemi1Anton Lavrinienko2Eugene Tukalenko3Tapio Mappes4Phillip C. Watts5Jaana Jurvansuu6Department of Ecology and Genetics, University of OuluDepartment of Ecology and Genetics, University of OuluDepartment of Ecology and Genetics, University of OuluInstitute of Biology and Medicine, Taras Shevchenko National University of KyivDepartment of Biological and Environmental Science, University of JyväskyläDepartment of Ecology and Genetics, University of OuluDepartment of Ecology and Genetics, University of OuluAbstract Background Elevated levels of environmental ionizing radiation can be a selective pressure for wildlife by producing reactive oxygen species and DNA damage. However, the underlying molecular mechanisms that are affected are not known. Results We isolated skin fibroblasts from bank voles (Myodes glareolus) inhabiting the Chernobyl nuclear power plant accident site where background radiation levels are about 100 times greater than in uncontaminated areas. After a 10 Gy dose of gamma radiation fibroblasts from Chernobyl animals recovered faster than fibroblasts isolated from bank voles living in uncontaminated control area. The Chernobyl fibroblasts were able to sustain significantly higher doses of an oxidant and they had, on average, a higher total antioxidant capacity than the control fibroblasts. Furthermore, the Chernobyl fibroblasts were also significantly more resistant than the control fibroblasts to continuous exposure to three DNA damaging drugs. After drug treatment transcription of p53-target gene pro-apoptotic Bax was higher in the control than in the Chernobyl fibroblasts. Conclusion Fibroblasts isolated from bank voles inhabiting Chernobyl nuclear power plant accident site show elevated antioxidant levels, lower sensitivity to apoptosis, and increased resistance against oxidative and DNA stresses. These cellular qualities may help bank voles inhabiting Chernobyl to cope with environmental radioactivity.http://link.springer.com/article/10.1186/s12860-018-0169-9Bank voleChernobylEnvironmental ionizing radiationp53DNA damageAntioxidant capacity |
| spellingShingle | Venla Mustonen Jenni Kesäniemi Anton Lavrinienko Eugene Tukalenko Tapio Mappes Phillip C. Watts Jaana Jurvansuu Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses BMC Cell Biology Bank vole Chernobyl Environmental ionizing radiation p53 DNA damage Antioxidant capacity |
| title | Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses |
| title_full | Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses |
| title_fullStr | Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses |
| title_full_unstemmed | Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses |
| title_short | Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses |
| title_sort | fibroblasts from bank voles inhabiting chernobyl have increased resistance against oxidative and dna stresses |
| topic | Bank vole Chernobyl Environmental ionizing radiation p53 DNA damage Antioxidant capacity |
| url | http://link.springer.com/article/10.1186/s12860-018-0169-9 |
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