Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance.
Alfalfa (Medicago sativa L.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abioti...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4422619?pdf=render |
| _version_ | 1830454988648742912 |
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| author | Zhi Wang Qingbo Ke Myoung Duck Kim Sun Ha Kim Chang Yoon Ji Jae Cheol Jeong Haeng-Soon Lee Woo Sung Park Mi-Jeong Ahn Hongbing Li Bingcheng Xu Xiping Deng Sang-Hoon Lee Yong Pyo Lim Sang-Soo Kwak |
| author_facet | Zhi Wang Qingbo Ke Myoung Duck Kim Sun Ha Kim Chang Yoon Ji Jae Cheol Jeong Haeng-Soon Lee Woo Sung Park Mi-Jeong Ahn Hongbing Li Bingcheng Xu Xiping Deng Sang-Hoon Lee Yong Pyo Lim Sang-Soo Kwak |
| author_sort | Zhi Wang |
| collection | DOAJ |
| description | Alfalfa (Medicago sativa L.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. In this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the IbOr gene into alfalfa (cv. Xinjiang Daye) under the control of an oxidative stress-inducible peroxidase (SWPA2) promoter through Agrobacterium tumefaciens-mediated transformation. Among the 11 transgenic alfalfa lines (referred to as SOR plants), three lines (SOR2, SOR3, and SOR8) selected based on their IbOr transcript levels were examined for their tolerance to methyl viologen (MV)-induced oxidative stress in a leaf disc assay. The SOR plants exhibited less damage in response to MV-mediated oxidative stress and salt stress than non-transgenic plants. The SOR plants also exhibited enhanced tolerance to drought stress, along with higher total carotenoid levels. The results suggest that SOR alfalfa plants would be useful as forage crops with improved nutritional value and increased tolerance to multiple abiotic stresses, which would enhance the development of sustainable agriculture on marginal lands. |
| first_indexed | 2024-12-21T09:34:39Z |
| format | Article |
| id | doaj.art-37dcf958cede4bc78b69d3a3acc28511 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T09:34:39Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-37dcf958cede4bc78b69d3a3acc285112022-12-21T19:08:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012605010.1371/journal.pone.0126050Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance.Zhi WangQingbo KeMyoung Duck KimSun Ha KimChang Yoon JiJae Cheol JeongHaeng-Soon LeeWoo Sung ParkMi-Jeong AhnHongbing LiBingcheng XuXiping DengSang-Hoon LeeYong Pyo LimSang-Soo KwakAlfalfa (Medicago sativa L.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. In this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the IbOr gene into alfalfa (cv. Xinjiang Daye) under the control of an oxidative stress-inducible peroxidase (SWPA2) promoter through Agrobacterium tumefaciens-mediated transformation. Among the 11 transgenic alfalfa lines (referred to as SOR plants), three lines (SOR2, SOR3, and SOR8) selected based on their IbOr transcript levels were examined for their tolerance to methyl viologen (MV)-induced oxidative stress in a leaf disc assay. The SOR plants exhibited less damage in response to MV-mediated oxidative stress and salt stress than non-transgenic plants. The SOR plants also exhibited enhanced tolerance to drought stress, along with higher total carotenoid levels. The results suggest that SOR alfalfa plants would be useful as forage crops with improved nutritional value and increased tolerance to multiple abiotic stresses, which would enhance the development of sustainable agriculture on marginal lands.http://europepmc.org/articles/PMC4422619?pdf=render |
| spellingShingle | Zhi Wang Qingbo Ke Myoung Duck Kim Sun Ha Kim Chang Yoon Ji Jae Cheol Jeong Haeng-Soon Lee Woo Sung Park Mi-Jeong Ahn Hongbing Li Bingcheng Xu Xiping Deng Sang-Hoon Lee Yong Pyo Lim Sang-Soo Kwak Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance. PLoS ONE |
| title | Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance. |
| title_full | Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance. |
| title_fullStr | Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance. |
| title_full_unstemmed | Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance. |
| title_short | Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance. |
| title_sort | transgenic alfalfa plants expressing the sweetpotato orange gene exhibit enhanced abiotic stress tolerance |
| url | http://europepmc.org/articles/PMC4422619?pdf=render |
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