Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth Factor
To evaluate the wound-healing effect of <i>Antheraea pernyi</i> epidermal growth factor (ApEGF), we performed the sequence analysis, cloning, and prokaryotic expression of cDNA from the ApEGF gene, examined the transcriptional changes, and investigated the wound-healing effect of this pr...
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MDPI AG
2022-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/11/975 |
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| author | Yu-Lan Piao Chun-Yang Zhang Yue Zhang Kun Qian Ying Zhou Jun-Yan Liu Young-Cheol Chang Hoon Cho Dubok Choi |
| author_facet | Yu-Lan Piao Chun-Yang Zhang Yue Zhang Kun Qian Ying Zhou Jun-Yan Liu Young-Cheol Chang Hoon Cho Dubok Choi |
| author_sort | Yu-Lan Piao |
| collection | DOAJ |
| description | To evaluate the wound-healing effect of <i>Antheraea pernyi</i> epidermal growth factor (ApEGF), we performed the sequence analysis, cloning, and prokaryotic expression of cDNA from the ApEGF gene, examined the transcriptional changes, and investigated the wound-healing effect of this protein in cells and rat epidermis. Primers were designed based on available sequence information related to the ApEGF gene in a public database, and part of the ApEGF sequence was obtained. The full-length cDNA sequence of ApEGF was obtained using inverse PCR. The gene sequence fragment of ApEGF was 666 bp in length, encoding 221 amino acids, with a predicted protein mass of 24.19 kD, an isoelectric point of 5.15, and no signal peptide sequence. Sequence homology analysis revealed 86.1% sequence homology with <i>Bombyx mori</i>, 92.7% with <i>Manducal sexta</i>, 92.6% with <i>Trichoplusia ni</i>, and 91.8% with <i>Helicoverpa armigera</i>. ApEGF was truncated and then subjected to prokaryotic expression, isolation, and purification. Truncated ApEGF was used for wound-healing experiments in vitro and in vivo. The results showed that after 48 h, transforming growth factor (TGF)-β1 had 187.32% cell growth effects, and the ApEGF group had 211.15% cell growth compared to the control group in vitro. In rat epidermis, truncated ApEGF showed a significantly better healing effect than the control. This result indicated that ApEGF, which exerted a direct wound-healing effect, could be used in wound-healing therapy. |
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| last_indexed | 2024-03-09T18:58:27Z |
| publishDate | 2022-10-01 |
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| series | Insects |
| spelling | doaj.art-37def83f44ef4203a78cea3c4206df6b2023-11-24T05:12:53ZengMDPI AGInsects2075-44502022-10-01131197510.3390/insects13110975Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth FactorYu-Lan Piao0Chun-Yang Zhang1Yue Zhang2Kun Qian3Ying Zhou4Jun-Yan Liu5Young-Cheol Chang6Hoon Cho7Dubok Choi8School of Food Engineering, Jilin Agriculture Science and Technology University, Jilin 132109, ChinaJilin Province Sericultural Scientific Research Institute, Jilin 132012, ChinaJilin Province Sericultural Scientific Research Institute, Jilin 132012, ChinaJilin Province Sericultural Scientific Research Institute, Jilin 132012, ChinaJilin Province Sericultural Scientific Research Institute, Jilin 132012, ChinaJilin Province Aikangshou Biotechnology Co., Ltd., Jilin 132012, ChinaCourse of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, Muroran 050-8585, JapanDepartment of Biochemical & Polymer Engineering, Chosun University, Gwangju 61452, KoreaFaculty of Advanced Industry Convergence, Chosun University, Gwangju 61452, KoreaTo evaluate the wound-healing effect of <i>Antheraea pernyi</i> epidermal growth factor (ApEGF), we performed the sequence analysis, cloning, and prokaryotic expression of cDNA from the ApEGF gene, examined the transcriptional changes, and investigated the wound-healing effect of this protein in cells and rat epidermis. Primers were designed based on available sequence information related to the ApEGF gene in a public database, and part of the ApEGF sequence was obtained. The full-length cDNA sequence of ApEGF was obtained using inverse PCR. The gene sequence fragment of ApEGF was 666 bp in length, encoding 221 amino acids, with a predicted protein mass of 24.19 kD, an isoelectric point of 5.15, and no signal peptide sequence. Sequence homology analysis revealed 86.1% sequence homology with <i>Bombyx mori</i>, 92.7% with <i>Manducal sexta</i>, 92.6% with <i>Trichoplusia ni</i>, and 91.8% with <i>Helicoverpa armigera</i>. ApEGF was truncated and then subjected to prokaryotic expression, isolation, and purification. Truncated ApEGF was used for wound-healing experiments in vitro and in vivo. The results showed that after 48 h, transforming growth factor (TGF)-β1 had 187.32% cell growth effects, and the ApEGF group had 211.15% cell growth compared to the control group in vitro. In rat epidermis, truncated ApEGF showed a significantly better healing effect than the control. This result indicated that ApEGF, which exerted a direct wound-healing effect, could be used in wound-healing therapy.https://www.mdpi.com/2075-4450/13/11/975wound healing<i>Antheraea pernyi</i>epidermal growth factorprokaryotic expressionre-epithelialization |
| spellingShingle | Yu-Lan Piao Chun-Yang Zhang Yue Zhang Kun Qian Ying Zhou Jun-Yan Liu Young-Cheol Chang Hoon Cho Dubok Choi Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth Factor Insects wound healing <i>Antheraea pernyi</i> epidermal growth factor prokaryotic expression re-epithelialization |
| title | Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth Factor |
| title_full | Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth Factor |
| title_fullStr | Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth Factor |
| title_full_unstemmed | Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth Factor |
| title_short | Wound-Healing Effect of <i>Antheraea pernyi</i> Epidermal Growth Factor |
| title_sort | wound healing effect of i antheraea pernyi i epidermal growth factor |
| topic | wound healing <i>Antheraea pernyi</i> epidermal growth factor prokaryotic expression re-epithelialization |
| url | https://www.mdpi.com/2075-4450/13/11/975 |
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