A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection
Hens infected with avian leukosis virus subgroup A (ALV-A) experience stunted growth, immunosuppression, and potentially, lymphoma development. According to past research, A20 can both promote and inhibit tumor growth. In this study, DF-1 cells were infected with ALV-A rHB2015012, and Gp85 expressio...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/10/2210 |
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| author | Xueyang Chen Xingming Wang Yuxin Yang Chun Fang Jing Liu Xiongyan Liang Yuying Yang |
| author_facet | Xueyang Chen Xingming Wang Yuxin Yang Chun Fang Jing Liu Xiongyan Liang Yuying Yang |
| author_sort | Xueyang Chen |
| collection | DOAJ |
| description | Hens infected with avian leukosis virus subgroup A (ALV-A) experience stunted growth, immunosuppression, and potentially, lymphoma development. According to past research, A20 can both promote and inhibit tumor growth. In this study, DF-1 cells were infected with ALV-A rHB2015012, and Gp85 expression was measured at various time points. A recombinant plasmid encoding the chicken A20 gene and short hairpin RNA targeting chicken A20 (A20-shRNA) was constructed and transfected into DF-1 cells to determine the effect on ALV-A replication. The potential signaling pathways of A20 were explored using bioinformatics prediction, co-immunoprecipitation, and other techniques. The results demonstrate that A20 and ALV-A promoted each other after ALV-A infection of DF-1 cells, upregulated A20, inhibited TRAF6 ubiquitination, and promoted STAT3 phosphorylation. The phosphorylated-STAT3 (p-STAT3) promoted the expression of proto-oncogene c-myc, which may lead to tumorigenesis. This study will help to further understand the tumorigenic process of ALV-A and provide a reference for preventing and controlling ALV. |
| first_indexed | 2024-03-09T19:22:45Z |
| format | Article |
| id | doaj.art-37e015be8bca45a89b4e070d58584df5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T19:22:45Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-37e015be8bca45a89b4e070d58584df52023-11-24T03:09:25ZengMDPI AGViruses1999-49152022-10-011410221010.3390/v14102210A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A InfectionXueyang Chen0Xingming Wang1Yuxin Yang2Chun Fang3Jing Liu4Xiongyan Liang5Yuying Yang6College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, ChinaCollege of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, ChinaCollege of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, ChinaCollege of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, ChinaCollege of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, ChinaCollege of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, ChinaCollege of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, ChinaHens infected with avian leukosis virus subgroup A (ALV-A) experience stunted growth, immunosuppression, and potentially, lymphoma development. According to past research, A20 can both promote and inhibit tumor growth. In this study, DF-1 cells were infected with ALV-A rHB2015012, and Gp85 expression was measured at various time points. A recombinant plasmid encoding the chicken A20 gene and short hairpin RNA targeting chicken A20 (A20-shRNA) was constructed and transfected into DF-1 cells to determine the effect on ALV-A replication. The potential signaling pathways of A20 were explored using bioinformatics prediction, co-immunoprecipitation, and other techniques. The results demonstrate that A20 and ALV-A promoted each other after ALV-A infection of DF-1 cells, upregulated A20, inhibited TRAF6 ubiquitination, and promoted STAT3 phosphorylation. The phosphorylated-STAT3 (p-STAT3) promoted the expression of proto-oncogene c-myc, which may lead to tumorigenesis. This study will help to further understand the tumorigenic process of ALV-A and provide a reference for preventing and controlling ALV.https://www.mdpi.com/1999-4915/14/10/2210ALV-Achicken A20TRAF6STAT3c-myctumorigenic mechanism |
| spellingShingle | Xueyang Chen Xingming Wang Yuxin Yang Chun Fang Jing Liu Xiongyan Liang Yuying Yang A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection Viruses ALV-A chicken A20 TRAF6 STAT3 c-myc tumorigenic mechanism |
| title | A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection |
| title_full | A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection |
| title_fullStr | A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection |
| title_full_unstemmed | A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection |
| title_short | A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection |
| title_sort | a20 enhances the expression of the proto oncogene c myc by downregulating traf6 ubiquitination after alv a infection |
| topic | ALV-A chicken A20 TRAF6 STAT3 c-myc tumorigenic mechanism |
| url | https://www.mdpi.com/1999-4915/14/10/2210 |
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