<i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis
Leptospirosis is an underestimated tropical disease caused by the pathogenic <i>Leptospira</i> species and responsible for several serious health problems. Here, we aimed to develop an ultrasensitive DNA biosensor for the rapid and on-site detection of the <i>Loa22</i> gene o...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/1424-8220/21/7/2552 |
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| author | Vivek Verma Deepak Kala Shagun Gupta Harsh Kumar Ankur Kaushal Kamil Kuča Natália Cruz-Martins Dinesh Kumar |
| author_facet | Vivek Verma Deepak Kala Shagun Gupta Harsh Kumar Ankur Kaushal Kamil Kuča Natália Cruz-Martins Dinesh Kumar |
| author_sort | Vivek Verma |
| collection | DOAJ |
| description | Leptospirosis is an underestimated tropical disease caused by the pathogenic <i>Leptospira</i> species and responsible for several serious health problems. Here, we aimed to develop an ultrasensitive DNA biosensor for the rapid and on-site detection of the <i>Loa22</i> gene of <i>Leptospira interrogans</i> using a gold nanoparticle–carbon nanofiber composite (AuN/CNF)-based screen-printed electrode. Cyclic voltammetry and electrochemical impedance were performed for electrochemical analysis. The sensitivity of the sensor was 5431.74 μA/cm<sup>2</sup>/ng with a LOD (detection limit) of 0.0077 ng/μL using cyclic voltammetry. The developed DNA biosensor was found highly specific to the <i>Loa22</i> gene of <i>L. interrogans</i>, with a storage stability at 4 °C for 180 days and a 6% loss of the initial response. This DNA-based sensor only takes 30 min for rapid detection of the pathogen, with a higher specificity and sensitivity. The promising results obtained suggest the application of the developed sensor as a point of care device for the diagnosis of leptospirosis. |
| first_indexed | 2024-03-10T12:34:39Z |
| format | Article |
| id | doaj.art-37e248181c9e47fc8864eac813cd4818 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T12:34:39Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-37e248181c9e47fc8864eac813cd48182023-11-21T14:20:11ZengMDPI AGSensors1424-82202021-04-01217255210.3390/s21072552<i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of LeptospirosisVivek Verma0Deepak Kala1Shagun Gupta2Harsh Kumar3Ankur Kaushal4Kamil Kuča5Natália Cruz-Martins6Dinesh Kumar7School of Bioengineering and Food Technology, Shoolini University, Solan 173229, HP, IndiaAmity Center of Nanotechnology, Amity University, Gurugram 122413, HR, IndiaCollege of Horticulture & Forestry-Neri, Hamirpur 177001, HP, IndiaSchool of Bioengineering and Food Technology, Shoolini University, Solan 173229, HP, IndiaAmity Center of Nanotechnology, Amity University, Gurugram 122413, HR, IndiaCenter for Basic and Applied Research, Faculty of Informatics and Management, University of Hradec Kralove, 50003 Hradec Kralove, Czech RepublicFaculty of Medicine, University of Porto, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, PortugalSchool of Bioengineering and Food Technology, Shoolini University, Solan 173229, HP, IndiaLeptospirosis is an underestimated tropical disease caused by the pathogenic <i>Leptospira</i> species and responsible for several serious health problems. Here, we aimed to develop an ultrasensitive DNA biosensor for the rapid and on-site detection of the <i>Loa22</i> gene of <i>Leptospira interrogans</i> using a gold nanoparticle–carbon nanofiber composite (AuN/CNF)-based screen-printed electrode. Cyclic voltammetry and electrochemical impedance were performed for electrochemical analysis. The sensitivity of the sensor was 5431.74 μA/cm<sup>2</sup>/ng with a LOD (detection limit) of 0.0077 ng/μL using cyclic voltammetry. The developed DNA biosensor was found highly specific to the <i>Loa22</i> gene of <i>L. interrogans</i>, with a storage stability at 4 °C for 180 days and a 6% loss of the initial response. This DNA-based sensor only takes 30 min for rapid detection of the pathogen, with a higher specificity and sensitivity. The promising results obtained suggest the application of the developed sensor as a point of care device for the diagnosis of leptospirosis.https://www.mdpi.com/1424-8220/21/7/2552DNA sensor<i>Leptospira interrogans</i><i>Loa22</i> geneAuN/CNF |
| spellingShingle | Vivek Verma Deepak Kala Shagun Gupta Harsh Kumar Ankur Kaushal Kamil Kuča Natália Cruz-Martins Dinesh Kumar <i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis Sensors DNA sensor <i>Leptospira interrogans</i> <i>Loa22</i> gene AuN/CNF |
| title | <i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis |
| title_full | <i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis |
| title_fullStr | <i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis |
| title_full_unstemmed | <i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis |
| title_short | <i>Leptospira interrogans</i> Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis |
| title_sort | i leptospira interrogans i outer membrane protein based nanohybrid sensor for the diagnosis of leptospirosis |
| topic | DNA sensor <i>Leptospira interrogans</i> <i>Loa22</i> gene AuN/CNF |
| url | https://www.mdpi.com/1424-8220/21/7/2552 |
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