Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats
In search of a new potent as an antioxidant from natural sources, plumieride—an iridoid isolated from the methanol extract of the bark of Plumeria bicolor (family Apocynaceae) was evaluated for its antioxidant potential against CCl4-induced peroxidative damage in liver of rats. The antioxidant pote...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2076-3921/3/4/798 |
| _version_ | 1827884027041808384 |
|---|---|
| author | Dharmendra Singh Priya Vrat Arya Ashutosh Sharma Ved Prakash Aggarwal Mahabeer Prasad Dobhal Radhey Shyam Gupta |
| author_facet | Dharmendra Singh Priya Vrat Arya Ashutosh Sharma Ved Prakash Aggarwal Mahabeer Prasad Dobhal Radhey Shyam Gupta |
| author_sort | Dharmendra Singh |
| collection | DOAJ |
| description | In search of a new potent as an antioxidant from natural sources, plumieride—an iridoid isolated from the methanol extract of the bark of Plumeria bicolor (family Apocynaceae) was evaluated for its antioxidant potential against CCl4-induced peroxidative damage in liver of rats. The antioxidant potential was evaluated by using hepatic tissue for SOD (superoxide dismutase), CAT (catalase), GSH (reduced glutathione), GPx (glutathione peroxidase), GR (glutathione reductase) and LPO (lipid peroxidation) alongwith the concomitant blood serum for AST & ALT (aspartate and alanine transaminases), GGT (gamma glutamyl transpeptidase), ALP (alkaline phosphatase), total bilirubin and total protein contents. All the biochemical parameters were significantly (p ≤ 0.001) altered by CCl4 (0.3 mL/kg body weight/twice a week, intra-peritoneally for 30 days). Simultaneously, oral treatment with plumieride (5, 10 and 20 mg/kg body weight/day for 30 days), restored all the parameters towards a normal level, remarkably. The histological findings of liver sections further corroborated the antioxidant potential of plumieride compared with standard drug-silymarin. In conclusion, plumieride consists of sugar molecules, which have alcoholic groups. Therefore, the alcoholic groups of sugar increase its antioxidant potential through intermolecular hydrogen bonding along with the thiol(SH) group of non-protein thiols and enzymes resulting in the restoration of the antioxidant system. Therefore, it might be considered a natural antioxidant against peroxidative damage in rats. |
| first_indexed | 2024-03-12T19:21:39Z |
| format | Article |
| id | doaj.art-37e6465d737148cb9a764200e3997d10 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T19:21:39Z |
| publishDate | 2014-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-37e6465d737148cb9a764200e3997d102023-08-02T05:08:14ZengMDPI AGAntioxidants2076-39212014-11-013479881310.3390/antiox3040798antiox3040798Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in RatsDharmendra Singh0Priya Vrat Arya1Ashutosh Sharma2Ved Prakash Aggarwal3Mahabeer Prasad Dobhal4Radhey Shyam Gupta5Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur 302 055, IndiaDepartment of Zoology, Dyal Singh College, University of Delhi, Lodhi Road, New Delhi 110 003, IndiaDepartment of Chemistry, University of Rajasthan, Jaipur 302 055, IndiaDepartment of Zoology, Dyal Singh College, University of Delhi, Lodhi Road, New Delhi 110 003, IndiaDepartment of Chemistry, University of Rajasthan, Jaipur 302 055, IndiaCentre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur 302 055, IndiaIn search of a new potent as an antioxidant from natural sources, plumieride—an iridoid isolated from the methanol extract of the bark of Plumeria bicolor (family Apocynaceae) was evaluated for its antioxidant potential against CCl4-induced peroxidative damage in liver of rats. The antioxidant potential was evaluated by using hepatic tissue for SOD (superoxide dismutase), CAT (catalase), GSH (reduced glutathione), GPx (glutathione peroxidase), GR (glutathione reductase) and LPO (lipid peroxidation) alongwith the concomitant blood serum for AST & ALT (aspartate and alanine transaminases), GGT (gamma glutamyl transpeptidase), ALP (alkaline phosphatase), total bilirubin and total protein contents. All the biochemical parameters were significantly (p ≤ 0.001) altered by CCl4 (0.3 mL/kg body weight/twice a week, intra-peritoneally for 30 days). Simultaneously, oral treatment with plumieride (5, 10 and 20 mg/kg body weight/day for 30 days), restored all the parameters towards a normal level, remarkably. The histological findings of liver sections further corroborated the antioxidant potential of plumieride compared with standard drug-silymarin. In conclusion, plumieride consists of sugar molecules, which have alcoholic groups. Therefore, the alcoholic groups of sugar increase its antioxidant potential through intermolecular hydrogen bonding along with the thiol(SH) group of non-protein thiols and enzymes resulting in the restoration of the antioxidant system. Therefore, it might be considered a natural antioxidant against peroxidative damage in rats.http://www.mdpi.com/2076-3921/3/4/798antioxidantscarbon tetrachloridefree radicalsmarker enzymesplumieride |
| spellingShingle | Dharmendra Singh Priya Vrat Arya Ashutosh Sharma Ved Prakash Aggarwal Mahabeer Prasad Dobhal Radhey Shyam Gupta Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats Antioxidants antioxidants carbon tetrachloride free radicals marker enzymes plumieride |
| title | Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats |
| title_full | Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats |
| title_fullStr | Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats |
| title_full_unstemmed | Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats |
| title_short | Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats |
| title_sort | antioxidant potential of plumieride against ccl4 induced peroxidative damage in rats |
| topic | antioxidants carbon tetrachloride free radicals marker enzymes plumieride |
| url | http://www.mdpi.com/2076-3921/3/4/798 |
| work_keys_str_mv | AT dharmendrasingh antioxidantpotentialofplumierideagainstccl4inducedperoxidativedamageinrats AT priyavratarya antioxidantpotentialofplumierideagainstccl4inducedperoxidativedamageinrats AT ashutoshsharma antioxidantpotentialofplumierideagainstccl4inducedperoxidativedamageinrats AT vedprakashaggarwal antioxidantpotentialofplumierideagainstccl4inducedperoxidativedamageinrats AT mahabeerprasaddobhal antioxidantpotentialofplumierideagainstccl4inducedperoxidativedamageinrats AT radheyshyamgupta antioxidantpotentialofplumierideagainstccl4inducedperoxidativedamageinrats |