In Vitro Growth of Human Follicles: Current and Future Perspectives
Ovarian tissue cryopreservation is gaining importance as a successful method to restore fertility to girls and young women at high risk of sterility. However, there are concerns regarding the safety of transplantation after ovarian tissue cryopreservation due to the high risk of reintroducing cancer...
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| Language: | English |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1510 |
| _version_ | 1797318723298656256 |
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| author | Clara Malo Sara Oliván Ignacio Ochoa Ariella Shikanov |
| author_facet | Clara Malo Sara Oliván Ignacio Ochoa Ariella Shikanov |
| author_sort | Clara Malo |
| collection | DOAJ |
| description | Ovarian tissue cryopreservation is gaining importance as a successful method to restore fertility to girls and young women at high risk of sterility. However, there are concerns regarding the safety of transplantation after ovarian tissue cryopreservation due to the high risk of reintroducing cancer cells and causing disease recurrence. In these cases, the development of culture systems that support oocyte development from the primordial follicle stage is required. Notable achievements have been reached in human follicle in vitro growth in the past decade. Currently, systems for the in vitro culture of ovarian tissue are based on two-dimensional substrates that do not support the survival of follicles or recapitulate the mechanical heterogenicity in the mammalian ovary. Recognition of the importance of special arrangements between cells has spurred research in three-dimensional culture systems, and the provision of a precise culture system that maximizes the diffusion of nutrients and gases through the follicles has raised interest in advanced biomimetic models. The current review critically examines various culture systems employed for the in vitro development of follicles, with a particular focus on solutions utilizing Organ-on-a-Chip (OOC) technology. The emphasis on OOC technology underscores its role as a promising avenue in ensuring the successful cultivation and maintenance of follicular structures during the culture period. |
| first_indexed | 2024-03-08T03:55:25Z |
| format | Article |
| id | doaj.art-37e8c1e0783c4f19a666f464d84cad65 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:55:25Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-37e8c1e0783c4f19a666f464d84cad652024-02-09T15:13:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253151010.3390/ijms25031510In Vitro Growth of Human Follicles: Current and Future PerspectivesClara Malo0Sara Oliván1Ignacio Ochoa2Ariella Shikanov3Tissue Microenvironment (TME) Lab, Aragón Institute of Engineering Research (I3A), University of Zaragoza, 50018 Zaragoza, SpainTissue Microenvironment (TME) Lab, Aragón Institute of Engineering Research (I3A), University of Zaragoza, 50018 Zaragoza, SpainTissue Microenvironment (TME) Lab, Aragón Institute of Engineering Research (I3A), University of Zaragoza, 50018 Zaragoza, SpainDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USAOvarian tissue cryopreservation is gaining importance as a successful method to restore fertility to girls and young women at high risk of sterility. However, there are concerns regarding the safety of transplantation after ovarian tissue cryopreservation due to the high risk of reintroducing cancer cells and causing disease recurrence. In these cases, the development of culture systems that support oocyte development from the primordial follicle stage is required. Notable achievements have been reached in human follicle in vitro growth in the past decade. Currently, systems for the in vitro culture of ovarian tissue are based on two-dimensional substrates that do not support the survival of follicles or recapitulate the mechanical heterogenicity in the mammalian ovary. Recognition of the importance of special arrangements between cells has spurred research in three-dimensional culture systems, and the provision of a precise culture system that maximizes the diffusion of nutrients and gases through the follicles has raised interest in advanced biomimetic models. The current review critically examines various culture systems employed for the in vitro development of follicles, with a particular focus on solutions utilizing Organ-on-a-Chip (OOC) technology. The emphasis on OOC technology underscores its role as a promising avenue in ensuring the successful cultivation and maintenance of follicular structures during the culture period.https://www.mdpi.com/1422-0067/25/3/1510cancercryopreservationfertility preservationin vitro follicular growthovarian tissueorgan-on-chip |
| spellingShingle | Clara Malo Sara Oliván Ignacio Ochoa Ariella Shikanov In Vitro Growth of Human Follicles: Current and Future Perspectives International Journal of Molecular Sciences cancer cryopreservation fertility preservation in vitro follicular growth ovarian tissue organ-on-chip |
| title | In Vitro Growth of Human Follicles: Current and Future Perspectives |
| title_full | In Vitro Growth of Human Follicles: Current and Future Perspectives |
| title_fullStr | In Vitro Growth of Human Follicles: Current and Future Perspectives |
| title_full_unstemmed | In Vitro Growth of Human Follicles: Current and Future Perspectives |
| title_short | In Vitro Growth of Human Follicles: Current and Future Perspectives |
| title_sort | in vitro growth of human follicles current and future perspectives |
| topic | cancer cryopreservation fertility preservation in vitro follicular growth ovarian tissue organ-on-chip |
| url | https://www.mdpi.com/1422-0067/25/3/1510 |
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