Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers
Pore-size distribution (PSD) is the most critical parameter for size-exclusion virus removal filters. Yet, different dry- and wet-state porometry methods yield different pore-size values. The goal of this work is to conduct comparative analysis of nitrogen gas sorption (NGSP), liquid-liquid and cryo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/9/1/1 |
| _version_ | 1797758288595517440 |
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| author | Simon Gustafsson Frank Westermann Tobias Hanrieder Laura Jung Horst Ruppach Albert Mihranyan |
| author_facet | Simon Gustafsson Frank Westermann Tobias Hanrieder Laura Jung Horst Ruppach Albert Mihranyan |
| author_sort | Simon Gustafsson |
| collection | DOAJ |
| description | Pore-size distribution (PSD) is the most critical parameter for size-exclusion virus removal filters. Yet, different dry- and wet-state porometry methods yield different pore-size values. The goal of this work is to conduct comparative analysis of nitrogen gas sorption (NGSP), liquid-liquid and cryoporometry with differential scanning calorimetry (CP-DSC) methods with respect to characterization of regular and cross-linked virus removal filter paper based on cellulose nanofibers, i.e. the mille-feuille filter. The filters were further characterized with atomic force and scanning electron microscopy. Finally, the removal of the worst-case model virus, i.e. minute virus of mice (MVM; 20 nm, nonenveloped parvovirus) was evaluated. The results revealed that there is no difference of the obtained PSDs between the wet methods, i.e. DSC and liquid-liquid porometry (LLP), as well as no difference between the regular and cross-linked filters regardless of method. MVM filtration at different trans membrane pressure (TMP) revealed strong dependence of the virus removal capability on applied pressure. It was further observed that cross-linking filters showed enhanced virus removal, especially at lower TMP. In all, the results of this study highlight the complex nature of virus capture in size-exclusion filters. |
| first_indexed | 2024-03-12T18:27:49Z |
| format | Article |
| id | doaj.art-37edd7358a0846459f6be3daee35e5ec |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-12T18:27:49Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-37edd7358a0846459f6be3daee35e5ec2023-08-02T08:26:41ZengMDPI AGMembranes2077-03752018-12-0191110.3390/membranes9010001membranes9010001Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter PapersSimon Gustafsson0Frank Westermann1Tobias Hanrieder2Laura Jung3Horst Ruppach4Albert Mihranyan5Division for Nanotechnology and Functional Materials, Department for Engineering Sciences, Uppsala University, Box 534, SE-751 21 Uppsala, SwedenCharles River Biopharmaceutical Services, Gottfried Hagen Str. 20, 51105 Köln, GermanyCharles River Biopharmaceutical Services, Gottfried Hagen Str. 20, 51105 Köln, GermanyCharles River Biopharmaceutical Services, Gottfried Hagen Str. 20, 51105 Köln, GermanyCharles River Biopharmaceutical Services, Gottfried Hagen Str. 20, 51105 Köln, GermanyDivision for Nanotechnology and Functional Materials, Department for Engineering Sciences, Uppsala University, Box 534, SE-751 21 Uppsala, SwedenPore-size distribution (PSD) is the most critical parameter for size-exclusion virus removal filters. Yet, different dry- and wet-state porometry methods yield different pore-size values. The goal of this work is to conduct comparative analysis of nitrogen gas sorption (NGSP), liquid-liquid and cryoporometry with differential scanning calorimetry (CP-DSC) methods with respect to characterization of regular and cross-linked virus removal filter paper based on cellulose nanofibers, i.e. the mille-feuille filter. The filters were further characterized with atomic force and scanning electron microscopy. Finally, the removal of the worst-case model virus, i.e. minute virus of mice (MVM; 20 nm, nonenveloped parvovirus) was evaluated. The results revealed that there is no difference of the obtained PSDs between the wet methods, i.e. DSC and liquid-liquid porometry (LLP), as well as no difference between the regular and cross-linked filters regardless of method. MVM filtration at different trans membrane pressure (TMP) revealed strong dependence of the virus removal capability on applied pressure. It was further observed that cross-linking filters showed enhanced virus removal, especially at lower TMP. In all, the results of this study highlight the complex nature of virus capture in size-exclusion filters.https://www.mdpi.com/2077-0375/9/1/1gas sorption porometryliquid-liquid porometrycryoporometrymille-feuille filter, parvovirusvirus removal filter |
| spellingShingle | Simon Gustafsson Frank Westermann Tobias Hanrieder Laura Jung Horst Ruppach Albert Mihranyan Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers Membranes gas sorption porometry liquid-liquid porometry cryoporometry mille-feuille filter, parvovirus virus removal filter |
| title | Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers |
| title_full | Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers |
| title_fullStr | Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers |
| title_full_unstemmed | Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers |
| title_short | Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers |
| title_sort | comparative analysis of dry and wet porometry methods for characterization of regular and cross linked virus removal filter papers |
| topic | gas sorption porometry liquid-liquid porometry cryoporometry mille-feuille filter, parvovirus virus removal filter |
| url | https://www.mdpi.com/2077-0375/9/1/1 |
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