Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract
Objective: To evaluate Brachychiton acerifolius leaf extracts as antidiabetic potential agent and to identify the main active constituents using bioactivity guided fractionation. Methods: In vitro antioxidant activity was evaluated for B. acerifolius different extracts using DPPH assay and vitamin C...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2017-05-01
|
| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169116306530 |
| _version_ | 1818024214694723584 |
|---|---|
| author | Aisha Hussein Abou Zeid Mohamed Ali Farag Manal Abdel Aziz Hamed Zeinab Abdel Aziz Kandil Radwa Hassan El-Akad Hanaa Mohamed El-Rafie |
| author_facet | Aisha Hussein Abou Zeid Mohamed Ali Farag Manal Abdel Aziz Hamed Zeinab Abdel Aziz Kandil Radwa Hassan El-Akad Hanaa Mohamed El-Rafie |
| author_sort | Aisha Hussein Abou Zeid |
| collection | DOAJ |
| description | Objective: To evaluate Brachychiton acerifolius leaf extracts as antidiabetic potential agent and to identify the main active constituents using bioactivity guided fractionation.
Methods: In vitro antioxidant activity was evaluated for B. acerifolius different extracts using DPPH assay and vitamin C as control. Antidiabetic activity was then determined using STZ-induced rats treated daily with ethyl acetate and 70% ethanol leaf extracts for 4 weeks at a dose of 200 g/kg body weight against gliclazide reference drug. Blood glucose, α-amylase, lipid profile, liver function enzymes and oxidative stress markers were assessed along with histopathological study for liver and pancreatic tissues. Isolation and structural elucidation of active compounds were made using Diaion and Sephadex followed by spectral analyses.
Results: The results indicated that ethyl acetate and ethanol leaf extracts exhibited the strongest antioxidant activity compared to that of vitamin C (IC50 0.05, 0.03 and 12 mg/mL, respectively). Both extracts showed potent anti-hyperglycemic activity evidenced by a significant decrease in serum glucose levels by 82.5% and 80.9% and α-amylase by 45.2% and 53.6%, as compared with gliclazide 68% and 59.4%, respectively. Fractionation of ethanol extract resulted in the isolation of 9 flavonoids including apigenin-7-O-α-rhamnosyl(1→2)-β-d-glucuronide, apigenin-7-O-β-d-glucuronide, apigenin-7-O-β-d-glucoside and luteolin-7-O-β-d-glucuronide.
Conclusions: This study highlights the potential use of B. acerifolius leaf extract enriched in flavones for the treatment of diabetes that would warrant further clinical trials investigation. |
| first_indexed | 2024-12-10T03:56:40Z |
| format | Article |
| id | doaj.art-37ef76358c294618a7e34fd6ecef4fa7 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-10T03:56:40Z |
| publishDate | 2017-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-37ef76358c294618a7e34fd6ecef4fa72022-12-22T02:03:05ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-05-017538939610.1016/j.apjtb.2017.01.009Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extractAisha Hussein Abou Zeid0Mohamed Ali Farag1Manal Abdel Aziz Hamed2Zeinab Abdel Aziz Kandil3Radwa Hassan El-Akad4Hanaa Mohamed El-Rafie5Pharmacognosy Department, National Research Centre, 33 El-Bohouth St. Former El-Tahrir St., P.O. 12622, Dokki, Giza, EgyptPharmacognosy Department, College of Pharmacy, Cairo University, Cairo, EgyptTherapeutic Chemistry Department, National Research Centre, 33 El-Bohouth St. Former El-Tahrir St., P.O. 12622, Dokki, Giza, EgyptPharmacognosy Department, College of Pharmacy, Cairo University, Cairo, EgyptPharmacognosy Department, National Research Centre, 33 El-Bohouth St. Former El-Tahrir St., P.O. 12622, Dokki, Giza, EgyptPharmacognosy Department, National Research Centre, 33 El-Bohouth St. Former El-Tahrir St., P.O. 12622, Dokki, Giza, EgyptObjective: To evaluate Brachychiton acerifolius leaf extracts as antidiabetic potential agent and to identify the main active constituents using bioactivity guided fractionation. Methods: In vitro antioxidant activity was evaluated for B. acerifolius different extracts using DPPH assay and vitamin C as control. Antidiabetic activity was then determined using STZ-induced rats treated daily with ethyl acetate and 70% ethanol leaf extracts for 4 weeks at a dose of 200 g/kg body weight against gliclazide reference drug. Blood glucose, α-amylase, lipid profile, liver function enzymes and oxidative stress markers were assessed along with histopathological study for liver and pancreatic tissues. Isolation and structural elucidation of active compounds were made using Diaion and Sephadex followed by spectral analyses. Results: The results indicated that ethyl acetate and ethanol leaf extracts exhibited the strongest antioxidant activity compared to that of vitamin C (IC50 0.05, 0.03 and 12 mg/mL, respectively). Both extracts showed potent anti-hyperglycemic activity evidenced by a significant decrease in serum glucose levels by 82.5% and 80.9% and α-amylase by 45.2% and 53.6%, as compared with gliclazide 68% and 59.4%, respectively. Fractionation of ethanol extract resulted in the isolation of 9 flavonoids including apigenin-7-O-α-rhamnosyl(1→2)-β-d-glucuronide, apigenin-7-O-β-d-glucuronide, apigenin-7-O-β-d-glucoside and luteolin-7-O-β-d-glucuronide. Conclusions: This study highlights the potential use of B. acerifolius leaf extract enriched in flavones for the treatment of diabetes that would warrant further clinical trials investigation.http://www.sciencedirect.com/science/article/pii/S2221169116306530STZ-induced diabetesDiaion fractionationFlavonoid glucuronidesBrachychiton acerifolius |
| spellingShingle | Aisha Hussein Abou Zeid Mohamed Ali Farag Manal Abdel Aziz Hamed Zeinab Abdel Aziz Kandil Radwa Hassan El-Akad Hanaa Mohamed El-Rafie Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract Asian Pacific Journal of Tropical Biomedicine STZ-induced diabetes Diaion fractionation Flavonoid glucuronides Brachychiton acerifolius |
| title | Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract |
| title_full | Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract |
| title_fullStr | Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract |
| title_full_unstemmed | Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract |
| title_short | Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract |
| title_sort | flavonoid chemical composition and antidiabetic potential of brachychiton acerifolius leaves extract |
| topic | STZ-induced diabetes Diaion fractionation Flavonoid glucuronides Brachychiton acerifolius |
| url | http://www.sciencedirect.com/science/article/pii/S2221169116306530 |
| work_keys_str_mv | AT aishahusseinabouzeid flavonoidchemicalcompositionandantidiabeticpotentialofbrachychitonacerifoliusleavesextract AT mohamedalifarag flavonoidchemicalcompositionandantidiabeticpotentialofbrachychitonacerifoliusleavesextract AT manalabdelazizhamed flavonoidchemicalcompositionandantidiabeticpotentialofbrachychitonacerifoliusleavesextract AT zeinababdelazizkandil flavonoidchemicalcompositionandantidiabeticpotentialofbrachychitonacerifoliusleavesextract AT radwahassanelakad flavonoidchemicalcompositionandantidiabeticpotentialofbrachychitonacerifoliusleavesextract AT hanaamohamedelrafie flavonoidchemicalcompositionandantidiabeticpotentialofbrachychitonacerifoliusleavesextract |