Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria
Antibacterial resistance is observed as a public health issue around the world. Every day, new resistance mechanisms appear and spread over the world. For that reason, it is imperative to improve the treatment schemes that have been developed to treat infections caused by wound infections, for insta...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/3/138 |
| _version_ | 1797486493315366912 |
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| author | Farah M. Abdulsada Nehia N. Hussein Ghassan M. Sulaiman Amer Al Ali Muhanad Alhujaily |
| author_facet | Farah M. Abdulsada Nehia N. Hussein Ghassan M. Sulaiman Amer Al Ali Muhanad Alhujaily |
| author_sort | Farah M. Abdulsada |
| collection | DOAJ |
| description | Antibacterial resistance is observed as a public health issue around the world. Every day, new resistance mechanisms appear and spread over the world. For that reason, it is imperative to improve the treatment schemes that have been developed to treat infections caused by wound infections, for instance, <i>Staphylococcus epidermidis</i> (<i>S. epidermidis</i>), <i>Proteus mirabilis</i> (<i>P. mirabilis)</i>, and <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>). In this case, we proposed a method that involves mixing the Gentamicin (Gen) with iron oxide nanoparticles (Fe<sub>3</sub>O<sub>4</sub> NPs) and a polymer (polyethylene glycol (PEG)) with Fe<sub>3</sub>O<sub>4</sub> NPs. X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), scanning electron microscope (SEM), and transmission electron microscope (TEM) were used to characterize Fe<sub>3</sub>O<sub>4</sub> NPs. Zeta potential and dynamic light scattering (DLS) were also assessed. The antibacterial activity of Fe<sub>3</sub>O<sub>4</sub> NPs, Fe<sub>3</sub>O<sub>4</sub> NPs+PEG, Fe<sub>3</sub>O<sub>4</sub> NPs+Gen, and Fe<sub>3</sub>O<sub>4</sub> NPs+PEG+Gen composites was investigated. The results showed a significant improvement in the antibacterial activity of nanoparticles against bacterial isolates, especially for the Fe<sub>3</sub>O<sub>4</sub> NPs+PEG+Gen as the diameter of the inhibition zone reached 26.33 ± 0.57 mm for <i>A. baumannii</i>, 25.66 ± 0.57 mm for <i>P. mirabilis,</i> and 23.66 ± 0.57 mm for <i>S. epidermidis</i>. The Fe<sub>3</sub>O<sub>4</sub> NPs, Fe<sub>3</sub>O<sub>4</sub> NPs+PEG, Fe<sub>3</sub>O<sub>4</sub>+Gen, and Fe<sub>3</sub>O<sub>4</sub>+PEG+Gen also showed effectiveness against the biofilm produced by these isolated bacteria. The minimum inhibitory concentration (MIC) of Fe<sub>3</sub>O<sub>4</sub> NPs for <i>S. epidermidis</i> was 25 µg mL<sup>−1</sup> and for <i>P. mirabilis</i> and <i>A. baumannii</i> was 50 µg mL<sup>−1</sup>. The findings suggest that the prepared nanoparticles could be potential therapeutic options for treating wound infections caused by <i>S. epidermidis</i>, <i>P. mirabilis,</i> and <i>A. baumannii</i>. |
| first_indexed | 2024-03-09T23:33:57Z |
| format | Article |
| id | doaj.art-37efc61533944c8ca9bbc8ccf0e916f4 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T23:33:57Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-37efc61533944c8ca9bbc8ccf0e916f42023-11-23T17:03:42ZengMDPI AGJournal of Functional Biomaterials2079-49832022-09-0113313810.3390/jfb13030138Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant BacteriaFarah M. Abdulsada0Nehia N. Hussein1Ghassan M. Sulaiman2Amer Al Ali3Muhanad Alhujaily4Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, 255, Bisha 67714, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, 255, Bisha 67714, Saudi ArabiaAntibacterial resistance is observed as a public health issue around the world. Every day, new resistance mechanisms appear and spread over the world. For that reason, it is imperative to improve the treatment schemes that have been developed to treat infections caused by wound infections, for instance, <i>Staphylococcus epidermidis</i> (<i>S. epidermidis</i>), <i>Proteus mirabilis</i> (<i>P. mirabilis)</i>, and <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>). In this case, we proposed a method that involves mixing the Gentamicin (Gen) with iron oxide nanoparticles (Fe<sub>3</sub>O<sub>4</sub> NPs) and a polymer (polyethylene glycol (PEG)) with Fe<sub>3</sub>O<sub>4</sub> NPs. X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), scanning electron microscope (SEM), and transmission electron microscope (TEM) were used to characterize Fe<sub>3</sub>O<sub>4</sub> NPs. Zeta potential and dynamic light scattering (DLS) were also assessed. The antibacterial activity of Fe<sub>3</sub>O<sub>4</sub> NPs, Fe<sub>3</sub>O<sub>4</sub> NPs+PEG, Fe<sub>3</sub>O<sub>4</sub> NPs+Gen, and Fe<sub>3</sub>O<sub>4</sub> NPs+PEG+Gen composites was investigated. The results showed a significant improvement in the antibacterial activity of nanoparticles against bacterial isolates, especially for the Fe<sub>3</sub>O<sub>4</sub> NPs+PEG+Gen as the diameter of the inhibition zone reached 26.33 ± 0.57 mm for <i>A. baumannii</i>, 25.66 ± 0.57 mm for <i>P. mirabilis,</i> and 23.66 ± 0.57 mm for <i>S. epidermidis</i>. The Fe<sub>3</sub>O<sub>4</sub> NPs, Fe<sub>3</sub>O<sub>4</sub> NPs+PEG, Fe<sub>3</sub>O<sub>4</sub>+Gen, and Fe<sub>3</sub>O<sub>4</sub>+PEG+Gen also showed effectiveness against the biofilm produced by these isolated bacteria. The minimum inhibitory concentration (MIC) of Fe<sub>3</sub>O<sub>4</sub> NPs for <i>S. epidermidis</i> was 25 µg mL<sup>−1</sup> and for <i>P. mirabilis</i> and <i>A. baumannii</i> was 50 µg mL<sup>−1</sup>. The findings suggest that the prepared nanoparticles could be potential therapeutic options for treating wound infections caused by <i>S. epidermidis</i>, <i>P. mirabilis,</i> and <i>A. baumannii</i>.https://www.mdpi.com/2079-4983/13/3/138pathogenic bacteriaantibacterial activitybiofilmPEGFe<sub>3</sub>O<sub>4</sub> nanoparticle |
| spellingShingle | Farah M. Abdulsada Nehia N. Hussein Ghassan M. Sulaiman Amer Al Ali Muhanad Alhujaily Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria Journal of Functional Biomaterials pathogenic bacteria antibacterial activity biofilm PEG Fe<sub>3</sub>O<sub>4</sub> nanoparticle |
| title | Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria |
| title_full | Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria |
| title_fullStr | Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria |
| title_full_unstemmed | Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria |
| title_short | Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria |
| title_sort | evaluation of the antibacterial properties of iron oxide polyethylene glycol and gentamicin conjugated nanoparticles against some multidrug resistant bacteria |
| topic | pathogenic bacteria antibacterial activity biofilm PEG Fe<sub>3</sub>O<sub>4</sub> nanoparticle |
| url | https://www.mdpi.com/2079-4983/13/3/138 |
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