Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis model
Background/purpose: Epigallocatechin-3-gallate (EGCG) is playing an increasingly important role in the treatment of oral diseases. However, its mechanisms remain to be clarified. This study aimed to investigate the effect of EGCG on oxidative and inflammatory stress and bone loss in experimental per...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790223000533 |
| _version_ | 1797672741083545600 |
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| author | Qin Fan Xiao-Hong Zhou Teng-Fei Wang Feng-Jiao Zeng Xia Liu Yu Gu Bin Chen Jie Yang Zi-Yi Pang Jian-Guo Liu Guo-Hui Bai |
| author_facet | Qin Fan Xiao-Hong Zhou Teng-Fei Wang Feng-Jiao Zeng Xia Liu Yu Gu Bin Chen Jie Yang Zi-Yi Pang Jian-Guo Liu Guo-Hui Bai |
| author_sort | Qin Fan |
| collection | DOAJ |
| description | Background/purpose: Epigallocatechin-3-gallate (EGCG) is playing an increasingly important role in the treatment of oral diseases. However, its mechanisms remain to be clarified. This study aimed to investigate the effect of EGCG on oxidative and inflammatory stress and bone loss in experimental periodontitis. Materials and methods: Periodontitis was induced in rats, followed by gavage using different concentrations of EGCG for 5 weeks. The levels of interleukin-1β (IL-1β), interleukin-18 (IL-18), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD) and malondialdehyde (MDA) in rats were measured. The degree of alveolar bone loss and the number of inflammatory cells were detected. The integrated optical density of nuclear factor erythroid 2-related factor (Nrf2), heme oxygenase-1 (HO-1), NLR pyrin domain-containing 3 (NLRP3) and nuclear factor-kappaB p65 (NF-κB p65) was measured. Results: EGCG (200 mg/kg) significantly reduced alveolar bone loss in the ligated maxillary molars and the number of inflammatory cells in the EGCG-200 group compared with the periodontitis, EGCG-100 and EGCG-400 groups. 200 mg/kg was the optimal dose of EGCG and was used in subsequent experiments. The expression levels of IL-1β, IL-18, TNF-α and MDA were significantly lower and the expression level of SOD was significantly higher in the EGCG-200 group compared with the periodontitis group. The expression of NLRP3 and NF-κB p65 was significantly decreased, while the expression of Nrf2 and HO-1 was significantly increased in the EGCG-200 group compared with the periodontitis group. Conclusion: These results suggest that EGCG inhibits oxidative stress and inflammatory responses in the periodontitis model by modulating the Nrf2/HO-1/NLRP3/NF-κB p65 signaling pathway, thereby decreasing alveolar bone loss. |
| first_indexed | 2024-03-11T21:34:39Z |
| format | Article |
| id | doaj.art-37f004b103334ebfa81555189fc624fb |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-03-11T21:34:39Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-37f004b103334ebfa81555189fc624fb2023-09-27T04:42:20ZengElsevierJournal of Dental Sciences1991-79022023-10-0118415671575Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis modelQin Fan0Xiao-Hong Zhou1Teng-Fei Wang2Feng-Jiao Zeng3Xia Liu4Yu Gu5Bin Chen6Jie Yang7Zi-Yi Pang8Jian-Guo Liu9Guo-Hui Bai10Affiliated Stomatological Hospital, Zunyi Medical University, Zunyi, China; Special Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, ChinaAffiliated Stomatological Hospital, Zunyi Medical University, Zunyi, ChinaSpecial Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, China; The Department of Stomatology, Chengdu Seventh People's Hospital, Chengdu, ChinaAffiliated Stomatological Hospital, Zunyi Medical University, Zunyi, ChinaAffiliated Stomatological Hospital, Zunyi Medical University, Zunyi, ChinaSpecial Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, China; Zhuhai Campus of Zunyi Medical University, Zhuhai, ChinaSpecial Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, ChinaSpecial Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, China; Hospital of Stomatology, Zunyi Medical University, Zunyi, ChinaSpecial Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, China; Hospital of Stomatology, Zunyi Medical University, Zunyi, ChinaAffiliated Stomatological Hospital, Zunyi Medical University, Zunyi, China; Special Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, China; Corresponding author. Affiliated Stomatological Hospital, Special Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, No.6 Xuefu West Road, Xinpu New District, Zunyi 563099, China.Affiliated Stomatological Hospital, Zunyi Medical University, Zunyi, China; Special Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, Zunyi, China; Corresponding author. Affiliated Stomatological Hospital, Special Key Laboratory of Oral Disease Research, Higher Education Institution in Guizhou Province, Zunyi Medical University, No.6 Xuefu West Road, Xinpu New District, Zunyi 563099, China.,Background/purpose: Epigallocatechin-3-gallate (EGCG) is playing an increasingly important role in the treatment of oral diseases. However, its mechanisms remain to be clarified. This study aimed to investigate the effect of EGCG on oxidative and inflammatory stress and bone loss in experimental periodontitis. Materials and methods: Periodontitis was induced in rats, followed by gavage using different concentrations of EGCG for 5 weeks. The levels of interleukin-1β (IL-1β), interleukin-18 (IL-18), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD) and malondialdehyde (MDA) in rats were measured. The degree of alveolar bone loss and the number of inflammatory cells were detected. The integrated optical density of nuclear factor erythroid 2-related factor (Nrf2), heme oxygenase-1 (HO-1), NLR pyrin domain-containing 3 (NLRP3) and nuclear factor-kappaB p65 (NF-κB p65) was measured. Results: EGCG (200 mg/kg) significantly reduced alveolar bone loss in the ligated maxillary molars and the number of inflammatory cells in the EGCG-200 group compared with the periodontitis, EGCG-100 and EGCG-400 groups. 200 mg/kg was the optimal dose of EGCG and was used in subsequent experiments. The expression levels of IL-1β, IL-18, TNF-α and MDA were significantly lower and the expression level of SOD was significantly higher in the EGCG-200 group compared with the periodontitis group. The expression of NLRP3 and NF-κB p65 was significantly decreased, while the expression of Nrf2 and HO-1 was significantly increased in the EGCG-200 group compared with the periodontitis group. Conclusion: These results suggest that EGCG inhibits oxidative stress and inflammatory responses in the periodontitis model by modulating the Nrf2/HO-1/NLRP3/NF-κB p65 signaling pathway, thereby decreasing alveolar bone loss.http://www.sciencedirect.com/science/article/pii/S1991790223000533Epigallocatechin-3-gallateHO-1NF-κB p65NLRP3Nrf2Periodontitis |
| spellingShingle | Qin Fan Xiao-Hong Zhou Teng-Fei Wang Feng-Jiao Zeng Xia Liu Yu Gu Bin Chen Jie Yang Zi-Yi Pang Jian-Guo Liu Guo-Hui Bai Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis model Journal of Dental Sciences Epigallocatechin-3-gallate HO-1 NF-κB p65 NLRP3 Nrf2 Periodontitis |
| title | Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis model |
| title_full | Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis model |
| title_fullStr | Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis model |
| title_full_unstemmed | Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis model |
| title_short | Effects of epigallocatechin-3-gallate on oxidative stress, inflammation, and bone loss in a rat periodontitis model |
| title_sort | effects of epigallocatechin 3 gallate on oxidative stress inflammation and bone loss in a rat periodontitis model |
| topic | Epigallocatechin-3-gallate HO-1 NF-κB p65 NLRP3 Nrf2 Periodontitis |
| url | http://www.sciencedirect.com/science/article/pii/S1991790223000533 |
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