Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite Markers
The Montenegrin sheep population mostly consists of local breeds and their crossbreeds that are very valuable from their genome preservation point of view. The aim of this study was the investigation of the genetic diversity of seven Montenegrin sheep breeds (Jezeropivska—JP, Sora—SOR, Zetska zuja—Z...
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MDPI AG
2022-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/21/3029 |
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| author | Milan Marković Dušica Radonjić Minja Zorc Milena Đokić Božidarka Marković |
| author_facet | Milan Marković Dušica Radonjić Minja Zorc Milena Đokić Božidarka Marković |
| author_sort | Milan Marković |
| collection | DOAJ |
| description | The Montenegrin sheep population mostly consists of local breeds and their crossbreeds that are very valuable from their genome preservation point of view. The aim of this study was the investigation of the genetic diversity of seven Montenegrin sheep breeds (Jezeropivska—JP, Sora—SOR, Zetska zuja—ZZ, Bardoka—BAR, Sjenička—SJ, Ljaba—Lj, and Piperska zuja—PIP) using 18 microsatellite sets of markers. The genotyping was done for 291 samples from seven populations using the multiplex amplification of sequences with polymerase chain reaction (PCR). The parameters of genetic diversity were estimated using several software tools. In total, 243 alleles were found, with a range of 6 to 25 by locus. The mean observed heterozygosity (Ho), polymorphism information content (PIC), and Fis values (fixation index) per marker were 0.728, 0.781, and −0.007, respectively. The mean number of alleles per breed varied from 4.889 in ZZ to 10.056 in JP. The highest Ho was estimated for JP (0.763) and the lowest for ZZ (0.640). The genetic structure showed close relations between SOR and JP, and both of them with SJ, while ZZ, LJ, and PIP were more distanced. This study provides useful indicators for the development of further in-depth studies and the creation of appropriate conservation programs. |
| first_indexed | 2024-03-09T19:19:53Z |
| format | Article |
| id | doaj.art-37f16f9aad5341b9977d7d6b5cacecfa |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T19:19:53Z |
| publishDate | 2022-11-01 |
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| series | Animals |
| spelling | doaj.art-37f16f9aad5341b9977d7d6b5cacecfa2023-11-24T03:25:56ZengMDPI AGAnimals2076-26152022-11-011221302910.3390/ani12213029Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite MarkersMilan Marković0Dušica Radonjić1Minja Zorc2Milena Đokić3Božidarka Marković4Biotechnical Faculty, University of Montenegro, Mihaila Lalića 15, 81000 Podgorica, MontenegroBiotechnical Faculty, University of Montenegro, Mihaila Lalića 15, 81000 Podgorica, MontenegroBiotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, SloveniaBiotechnical Faculty, University of Montenegro, Mihaila Lalića 15, 81000 Podgorica, MontenegroBiotechnical Faculty, University of Montenegro, Mihaila Lalića 15, 81000 Podgorica, MontenegroThe Montenegrin sheep population mostly consists of local breeds and their crossbreeds that are very valuable from their genome preservation point of view. The aim of this study was the investigation of the genetic diversity of seven Montenegrin sheep breeds (Jezeropivska—JP, Sora—SOR, Zetska zuja—ZZ, Bardoka—BAR, Sjenička—SJ, Ljaba—Lj, and Piperska zuja—PIP) using 18 microsatellite sets of markers. The genotyping was done for 291 samples from seven populations using the multiplex amplification of sequences with polymerase chain reaction (PCR). The parameters of genetic diversity were estimated using several software tools. In total, 243 alleles were found, with a range of 6 to 25 by locus. The mean observed heterozygosity (Ho), polymorphism information content (PIC), and Fis values (fixation index) per marker were 0.728, 0.781, and −0.007, respectively. The mean number of alleles per breed varied from 4.889 in ZZ to 10.056 in JP. The highest Ho was estimated for JP (0.763) and the lowest for ZZ (0.640). The genetic structure showed close relations between SOR and JP, and both of them with SJ, while ZZ, LJ, and PIP were more distanced. This study provides useful indicators for the development of further in-depth studies and the creation of appropriate conservation programs.https://www.mdpi.com/2076-2615/12/21/3029local breedsmicrosatellitesgenetic diversitypopulation structure |
| spellingShingle | Milan Marković Dušica Radonjić Minja Zorc Milena Đokić Božidarka Marković Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite Markers Animals local breeds microsatellites genetic diversity population structure |
| title | Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite Markers |
| title_full | Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite Markers |
| title_fullStr | Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite Markers |
| title_full_unstemmed | Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite Markers |
| title_short | Genetic Diversity of Montenegrin Local Sheep Breeds Based on Microsatellite Markers |
| title_sort | genetic diversity of montenegrin local sheep breeds based on microsatellite markers |
| topic | local breeds microsatellites genetic diversity population structure |
| url | https://www.mdpi.com/2076-2615/12/21/3029 |
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