Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells
Astragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown. This study aimed to investigate expression of inflammatory cytokines...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-03-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/17/3/3155/ |
| _version_ | 1830404519221002240 |
|---|---|
| author | Aiping Lu Cheng Lu Li Xu Jun Shu Xiaojuan He |
| author_facet | Aiping Lu Cheng Lu Li Xu Jun Shu Xiaojuan He |
| author_sort | Aiping Lu |
| collection | DOAJ |
| description | Astragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown. This study aimed to investigate expression of inflammatory cytokines and the MAPK/NF-κB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS). The results showed that the concentrations of TNF-a and IL-1β released from LPS stimulated THP-1 cells increased significantly compared to control (p < 0.01). After treatment with APS, the TNF-a and IL-1β levels were significantly lower than those in the LPS group (p < 0.05). The mRNA expression of TNF-a and IL-1β were also inhibited. Mechanistic studies indicated that APS strongly suppressed NF-κB activation and down-regulated the phosphorylation of ERK and JNK, which are important signaling pathways involved in the production of TNF-a and IL-1β, demonstrating that APS could suppress the production of TNF-a and IL-1β by LPS stimulated macrophages by inhibiting NF-κB activation and ERK and JNK phosphorylation. |
| first_indexed | 2024-12-20T17:27:06Z |
| format | Article |
| id | doaj.art-37f99d67ad624923b300bd4d5436afc6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T17:27:06Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-37f99d67ad624923b300bd4d5436afc62022-12-21T19:31:30ZengMDPI AGMolecules1420-30492012-03-011733155316410.3390/molecules17033155Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 CellsAiping LuCheng LuLi XuJun ShuXiaojuan HeAstragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown. This study aimed to investigate expression of inflammatory cytokines and the MAPK/NF-κB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS). The results showed that the concentrations of TNF-a and IL-1β released from LPS stimulated THP-1 cells increased significantly compared to control (p < 0.01). After treatment with APS, the TNF-a and IL-1β levels were significantly lower than those in the LPS group (p < 0.05). The mRNA expression of TNF-a and IL-1β were also inhibited. Mechanistic studies indicated that APS strongly suppressed NF-κB activation and down-regulated the phosphorylation of ERK and JNK, which are important signaling pathways involved in the production of TNF-a and IL-1β, demonstrating that APS could suppress the production of TNF-a and IL-1β by LPS stimulated macrophages by inhibiting NF-κB activation and ERK and JNK phosphorylation.http://www.mdpi.com/1420-3049/17/3/3155/Astragalus polysaccharidesanti-inflammatorymacrophageMAPKNF-κB |
| spellingShingle | Aiping Lu Cheng Lu Li Xu Jun Shu Xiaojuan He Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells Molecules Astragalus polysaccharides anti-inflammatory macrophage MAPK NF-κB |
| title | Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells |
| title_full | Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells |
| title_fullStr | Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells |
| title_full_unstemmed | Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells |
| title_short | Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells |
| title_sort | inhibitory effect of astragalus polysaccharides on lipopolysaccharide induced tnf a and il 1β production in thp 1 cells |
| topic | Astragalus polysaccharides anti-inflammatory macrophage MAPK NF-κB |
| url | http://www.mdpi.com/1420-3049/17/3/3155/ |
| work_keys_str_mv | AT aipinglu inhibitoryeffectofastragaluspolysaccharidesonlipopolysaccharideinducedtnfaandil1bproductioninthp1cells AT chenglu inhibitoryeffectofastragaluspolysaccharidesonlipopolysaccharideinducedtnfaandil1bproductioninthp1cells AT lixu inhibitoryeffectofastragaluspolysaccharidesonlipopolysaccharideinducedtnfaandil1bproductioninthp1cells AT junshu inhibitoryeffectofastragaluspolysaccharidesonlipopolysaccharideinducedtnfaandil1bproductioninthp1cells AT xiaojuanhe inhibitoryeffectofastragaluspolysaccharidesonlipopolysaccharideinducedtnfaandil1bproductioninthp1cells |