Phage-antibiotic synergy reduces Burkholderia cenocepacia population
Abstract Background Burkholderia cenocepacia is an opportunistic pathogen that can cause acute and chronic infections in patients with weakened immune systems and in patients with cystic fibrosis. B. cenocepacia is resistant to many antibiotics making treatment challenging. Consequently, there is a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02738-0 |
| _version_ | 1797958794712449024 |
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| author | Anna G. Mankovich Kristen Maciel Madison Kavanaugh Erin Kistler Emily Muckle Christine L. Weingart |
| author_facet | Anna G. Mankovich Kristen Maciel Madison Kavanaugh Erin Kistler Emily Muckle Christine L. Weingart |
| author_sort | Anna G. Mankovich |
| collection | DOAJ |
| description | Abstract Background Burkholderia cenocepacia is an opportunistic pathogen that can cause acute and chronic infections in patients with weakened immune systems and in patients with cystic fibrosis. B. cenocepacia is resistant to many antibiotics making treatment challenging. Consequently, there is a critical need for alternative strategies to treat B. cenocepacia infections such as using bacteriophages and/or bacteriophages with subinhibitory doses of antibiotic called phage-antibiotic synergy. Results We isolated a bacteriophage, KP1, from raw sewage that infects B. cenocepacia. Its morphological characteristics indicate it belongs in the family Siphoviridae, it has a 52 Kb ds DNA genome, and it has a narrow host range. We determined it rescued infections in Lemna minor (duckweed) and moderately reduced bacterial populations in our artificial sputum medium model. Conclusion These results suggest that KP1 phage alone in the duckweed model or in combination with antibiotics in the ASMDM model improves the efficacy of reducing B. cenocepacia populations. |
| first_indexed | 2024-04-11T00:23:49Z |
| format | Article |
| id | doaj.art-37fa117c672147b9a21d65c1896b5425 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-11T00:23:49Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-37fa117c672147b9a21d65c1896b54252023-01-08T12:06:07ZengBMCBMC Microbiology1471-21802023-01-0123111210.1186/s12866-022-02738-0Phage-antibiotic synergy reduces Burkholderia cenocepacia populationAnna G. Mankovich0Kristen Maciel1Madison Kavanaugh2Erin Kistler3Emily Muckle4Christine L. Weingart5Department of Cell and Developmental Biology, University of Illinois Urbana-ChampaignSoutheast Denver PediatricsDepartment of Pediatrics, UPMC Children’s Hospital of PittsburghDepartment of Biology, Denison UniversityDepartment of Biology, Denison UniversityDepartment of Biology, Denison UniversityAbstract Background Burkholderia cenocepacia is an opportunistic pathogen that can cause acute and chronic infections in patients with weakened immune systems and in patients with cystic fibrosis. B. cenocepacia is resistant to many antibiotics making treatment challenging. Consequently, there is a critical need for alternative strategies to treat B. cenocepacia infections such as using bacteriophages and/or bacteriophages with subinhibitory doses of antibiotic called phage-antibiotic synergy. Results We isolated a bacteriophage, KP1, from raw sewage that infects B. cenocepacia. Its morphological characteristics indicate it belongs in the family Siphoviridae, it has a 52 Kb ds DNA genome, and it has a narrow host range. We determined it rescued infections in Lemna minor (duckweed) and moderately reduced bacterial populations in our artificial sputum medium model. Conclusion These results suggest that KP1 phage alone in the duckweed model or in combination with antibiotics in the ASMDM model improves the efficacy of reducing B. cenocepacia populations.https://doi.org/10.1186/s12866-022-02738-0BurkholderiacenocepaciaBacteriophagePhage-antibiotic therapyASMDM model |
| spellingShingle | Anna G. Mankovich Kristen Maciel Madison Kavanaugh Erin Kistler Emily Muckle Christine L. Weingart Phage-antibiotic synergy reduces Burkholderia cenocepacia population BMC Microbiology Burkholderia cenocepacia Bacteriophage Phage-antibiotic therapy ASMDM model |
| title | Phage-antibiotic synergy reduces Burkholderia cenocepacia population |
| title_full | Phage-antibiotic synergy reduces Burkholderia cenocepacia population |
| title_fullStr | Phage-antibiotic synergy reduces Burkholderia cenocepacia population |
| title_full_unstemmed | Phage-antibiotic synergy reduces Burkholderia cenocepacia population |
| title_short | Phage-antibiotic synergy reduces Burkholderia cenocepacia population |
| title_sort | phage antibiotic synergy reduces burkholderia cenocepacia population |
| topic | Burkholderia cenocepacia Bacteriophage Phage-antibiotic therapy ASMDM model |
| url | https://doi.org/10.1186/s12866-022-02738-0 |
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