The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection.
The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4492946?pdf=render |
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| author | Brittany E Goldberg Emmanuel F Mongodin Cheron E Jones Michelle Chung Claire M Fraser Anupama Tate Steven L Zeichner |
| author_facet | Brittany E Goldberg Emmanuel F Mongodin Cheron E Jones Michelle Chung Claire M Fraser Anupama Tate Steven L Zeichner |
| author_sort | Brittany E Goldberg |
| collection | DOAJ |
| description | The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed population, the spectrum of potential oral disease is even broader, encompassing candidiasis, necrotizing gingivitis, parotid gland enlargement, Kaposi's sarcoma, oral warts and other diseases. Here, we used 454 pyrosequencing of bacterial 16S rRNA genes to examine the oral microbiome of saliva, mucosal and tooth samples from HIV-positive and negative children. Patient demographics and clinical characteristics were collected from a cross-section of patients undergoing routine dental care. Multiple specimens from different sampling sites in the mouth were collected for each patient. The goal of the study was to observe the potential diversity of the oral microbiota among individual patients, sample locations, HIV status and various dental characteristics. We found that there were significant differences in the microbiome among the enrolled patients, and between sampling locations. The analysis was complicated by uneven enrollment in the patient cohorts, with only five HIV-negative patients enrolled in the study and by the rapid improvement in the health of HIV-infected children between the time the study was conceived and completed. The generally good oral health of the HIV-negative patients limited the number of dental plaque samples that could be collected. We did not identify significant differences between well-controlled HIV-positive patients and HIV-negative controls, suggesting that well-controlled HIV-positive patients essentially harbor similar oral flora compared to patients without HIV. Nor were significant differences in the oral microbiota identified between different teeth or with different dental characteristics. Additional studies are needed to better characterize the oral microbiome in children and those with poorly-controlled HIV infections. |
| first_indexed | 2024-12-14T19:53:34Z |
| format | Article |
| id | doaj.art-37fdb18099b54581b230eef118d2c9dd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T19:53:34Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-37fdb18099b54581b230eef118d2c9dd2022-12-21T22:49:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013161510.1371/journal.pone.0131615The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection.Brittany E GoldbergEmmanuel F MongodinCheron E JonesMichelle ChungClaire M FraserAnupama TateSteven L ZeichnerThe oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed population, the spectrum of potential oral disease is even broader, encompassing candidiasis, necrotizing gingivitis, parotid gland enlargement, Kaposi's sarcoma, oral warts and other diseases. Here, we used 454 pyrosequencing of bacterial 16S rRNA genes to examine the oral microbiome of saliva, mucosal and tooth samples from HIV-positive and negative children. Patient demographics and clinical characteristics were collected from a cross-section of patients undergoing routine dental care. Multiple specimens from different sampling sites in the mouth were collected for each patient. The goal of the study was to observe the potential diversity of the oral microbiota among individual patients, sample locations, HIV status and various dental characteristics. We found that there were significant differences in the microbiome among the enrolled patients, and between sampling locations. The analysis was complicated by uneven enrollment in the patient cohorts, with only five HIV-negative patients enrolled in the study and by the rapid improvement in the health of HIV-infected children between the time the study was conceived and completed. The generally good oral health of the HIV-negative patients limited the number of dental plaque samples that could be collected. We did not identify significant differences between well-controlled HIV-positive patients and HIV-negative controls, suggesting that well-controlled HIV-positive patients essentially harbor similar oral flora compared to patients without HIV. Nor were significant differences in the oral microbiota identified between different teeth or with different dental characteristics. Additional studies are needed to better characterize the oral microbiome in children and those with poorly-controlled HIV infections.http://europepmc.org/articles/PMC4492946?pdf=render |
| spellingShingle | Brittany E Goldberg Emmanuel F Mongodin Cheron E Jones Michelle Chung Claire M Fraser Anupama Tate Steven L Zeichner The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection. PLoS ONE |
| title | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection. |
| title_full | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection. |
| title_fullStr | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection. |
| title_full_unstemmed | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection. |
| title_short | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection. |
| title_sort | oral bacterial communities of children with well controlled hiv infection and without hiv infection |
| url | http://europepmc.org/articles/PMC4492946?pdf=render |
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