Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis
The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx ana...
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MDPI AG
2014-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/15/3/4583 |
| _version_ | 1811322589968072704 |
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| author | Yanpeng Dong Guoqiang Fan Zhenli Zhao Minjie Deng |
| author_facet | Yanpeng Dong Guoqiang Fan Zhenli Zhao Minjie Deng |
| author_sort | Yanpeng Dong |
| collection | DOAJ |
| description | The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might be involved in this plant’s response to water deficit. Potted seedlings were subjected to well-watered conditions and drought stress, respectively. More than 290 million raw transcript reads were assembled into 111,660 unigenes, with a mean length of 1013 bp. Clusters of orthologous groups, gene ontology and the Kyoto Encyclopedia of Genes and Genomes annotations analyses were performed on the unigenes. Many differentially expressed genes and several metabolic pathways were identified. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of 14 genes. Our study identified altered gene expression in P. australis induced by drought stress and provided a comprehensive map of drought-responsive genes and pathways in this species. To our knowledge, this is the first publicly available global transcriptome study of P. australis. This study provides a valuable genetic resource for this species. |
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| format | Article |
| id | doaj.art-37fe5833c98647f1b32f6739a5c84e64 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T13:38:56Z |
| publishDate | 2014-03-01 |
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| spelling | doaj.art-37fe5833c98647f1b32f6739a5c84e642022-12-22T02:44:42ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-03-011534583460710.3390/ijms15034583ijms15034583Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australisYanpeng Dong0Guoqiang Fan1Zhenli Zhao2Minjie Deng3Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, ChinaInstitute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, ChinaInstitute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, ChinaCollege of Forestry, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, ChinaThe response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might be involved in this plant’s response to water deficit. Potted seedlings were subjected to well-watered conditions and drought stress, respectively. More than 290 million raw transcript reads were assembled into 111,660 unigenes, with a mean length of 1013 bp. Clusters of orthologous groups, gene ontology and the Kyoto Encyclopedia of Genes and Genomes annotations analyses were performed on the unigenes. Many differentially expressed genes and several metabolic pathways were identified. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of 14 genes. Our study identified altered gene expression in P. australis induced by drought stress and provided a comprehensive map of drought-responsive genes and pathways in this species. To our knowledge, this is the first publicly available global transcriptome study of P. australis. This study provides a valuable genetic resource for this species.http://www.mdpi.com/1422-0067/15/3/4583droughtdiploidautotetraploidtranscriptome |
| spellingShingle | Yanpeng Dong Guoqiang Fan Zhenli Zhao Minjie Deng Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis International Journal of Molecular Sciences drought diploid autotetraploid transcriptome |
| title | Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis |
| title_full | Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis |
| title_fullStr | Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis |
| title_full_unstemmed | Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis |
| title_short | Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis |
| title_sort | transcriptome expression profiling in response to drought stress in paulownia australis |
| topic | drought diploid autotetraploid transcriptome |
| url | http://www.mdpi.com/1422-0067/15/3/4583 |
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