The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens
Summary: This study aims to determine the influence of silver nanoparticles (Ag-NPs) on performance, clinical and pathological pictures, virulence gene expression, and electron microscopy of experimentally induced Escherichia coli infection (colisepticemia) in broiler chickens. One-day-old chickens...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-06-01
|
| Series: | Journal of Applied Poultry Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1056617121000180 |
| _version_ | 1818600463355871232 |
|---|---|
| author | M.H.H. Awaad K. M.El. Moustafa S.A. Zoulfakar M.S. Elhalawany F.F. Mohammed R.M. El-Refay E.A. Morsy |
| author_facet | M.H.H. Awaad K. M.El. Moustafa S.A. Zoulfakar M.S. Elhalawany F.F. Mohammed R.M. El-Refay E.A. Morsy |
| author_sort | M.H.H. Awaad |
| collection | DOAJ |
| description | Summary: This study aims to determine the influence of silver nanoparticles (Ag-NPs) on performance, clinical and pathological pictures, virulence gene expression, and electron microscopy of experimentally induced Escherichia coli infection (colisepticemia) in broiler chickens. One-day-old chickens (n = 120) were segregated into 4 equal groups (1–4) with 3 replicates of 10 birds each. Birds were fed mash diet in 3 phases: starter (1–14 d), grower (15–28 d), and finisher (29–35 d). Chickens of the first 3 groups (T1, T2, T3) were fed Ag-NPs in the following dietary levels: 4, 6, and 8 mg/kg diet, respectively, while chickens of the fourth group (C) were fed a basal diet. All groups were individually infected by crop gavages with 0.5 mL/bird of PBS containing 3 × 108 CFU/mL of E. coli serogroup O78 for 2 successive days. In vitro Ag-NPs had a potent inhibitory effect against E. coli growth in pure form only (2,000 mg). In vivo study revealed that treatment of colisepticemic broiler chickens at a low dose (4 mg/kg diet) improved performance, reduced both gross and histopathological lesion scores, and virulence genes. It also altered the electron microscopic profile of the inoculated E. coli in the examined liver tissue. Severe negative effect was recorded on supplementation of the higher dose of Ag-NPs (8 mg/kg diet) that resulted in major effects on all the measured parameters except the gene expression analysis. Ag-NPs treatment with a dose of 4 mg/kg diet could play an optimistic role in the impedance of colisepticemia in broiler chickens. |
| first_indexed | 2024-12-16T12:35:53Z |
| format | Article |
| id | doaj.art-3800b576598042c1a379b5702ac614a2 |
| institution | Directory Open Access Journal |
| issn | 1056-6171 |
| language | English |
| last_indexed | 2024-12-16T12:35:53Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Applied Poultry Research |
| spelling | doaj.art-3800b576598042c1a379b5702ac614a22022-12-21T22:31:34ZengElsevierJournal of Applied Poultry Research1056-61712021-06-01302100155The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickensM.H.H. Awaad0K. M.El. Moustafa1S.A. Zoulfakar2M.S. Elhalawany3F.F. Mohammed4R.M. El-Refay5E.A. Morsy6Faculty of Veterinary Medicine, Cairo University, Giza, EgyptAnimal Production Research Institute, Agriculture Research Center, Giza, EgyptFaculty of Veterinary Medicine, Cairo University, Giza, EgyptFaculty of Science, Cairo University, Giza, EgyptFaculty of Veterinary Medicine, Cairo University, Giza, EgyptFaculty of Veterinary Medicine, Cairo University, Giza, EgyptFaculty of Veterinary Medicine, Cairo University, Giza, Egypt; Corresponding author:Summary: This study aims to determine the influence of silver nanoparticles (Ag-NPs) on performance, clinical and pathological pictures, virulence gene expression, and electron microscopy of experimentally induced Escherichia coli infection (colisepticemia) in broiler chickens. One-day-old chickens (n = 120) were segregated into 4 equal groups (1–4) with 3 replicates of 10 birds each. Birds were fed mash diet in 3 phases: starter (1–14 d), grower (15–28 d), and finisher (29–35 d). Chickens of the first 3 groups (T1, T2, T3) were fed Ag-NPs in the following dietary levels: 4, 6, and 8 mg/kg diet, respectively, while chickens of the fourth group (C) were fed a basal diet. All groups were individually infected by crop gavages with 0.5 mL/bird of PBS containing 3 × 108 CFU/mL of E. coli serogroup O78 for 2 successive days. In vitro Ag-NPs had a potent inhibitory effect against E. coli growth in pure form only (2,000 mg). In vivo study revealed that treatment of colisepticemic broiler chickens at a low dose (4 mg/kg diet) improved performance, reduced both gross and histopathological lesion scores, and virulence genes. It also altered the electron microscopic profile of the inoculated E. coli in the examined liver tissue. Severe negative effect was recorded on supplementation of the higher dose of Ag-NPs (8 mg/kg diet) that resulted in major effects on all the measured parameters except the gene expression analysis. Ag-NPs treatment with a dose of 4 mg/kg diet could play an optimistic role in the impedance of colisepticemia in broiler chickens.http://www.sciencedirect.com/science/article/pii/S1056617121000180silver nanoparticlechicken performancecolisepticemiaE. coli electron microscopyE. coli virulence gene |
| spellingShingle | M.H.H. Awaad K. M.El. Moustafa S.A. Zoulfakar M.S. Elhalawany F.F. Mohammed R.M. El-Refay E.A. Morsy The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens Journal of Applied Poultry Research silver nanoparticle chicken performance colisepticemia E. coli electron microscopy E. coli virulence gene |
| title | The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens |
| title_full | The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens |
| title_fullStr | The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens |
| title_full_unstemmed | The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens |
| title_short | The role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens |
| title_sort | role of silver nanoparticles in the reluctance of colisepticemia in broiler chickens |
| topic | silver nanoparticle chicken performance colisepticemia E. coli electron microscopy E. coli virulence gene |
| url | http://www.sciencedirect.com/science/article/pii/S1056617121000180 |
| work_keys_str_mv | AT mhhawaad theroleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT kmelmoustafa theroleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT sazoulfakar theroleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT mselhalawany theroleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT ffmohammed theroleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT rmelrefay theroleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT eamorsy theroleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT mhhawaad roleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT kmelmoustafa roleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT sazoulfakar roleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT mselhalawany roleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT ffmohammed roleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT rmelrefay roleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens AT eamorsy roleofsilvernanoparticlesinthereluctanceofcolisepticemiainbroilerchickens |