Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis
ABSTRACT Introduction: Sepsis remains a major cause of death in critically ill patients in Indian population because of high susceptibility towards infectious diseases in the world. Procalcitonin (PCT) is a well-studied marker in foreign countries but needs to be established in Indian populatio...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2020-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/14053/45261_CE[Ra1]_F(SL)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
| Summary: | ABSTRACT
Introduction: Sepsis remains a major cause of death in critically
ill patients in Indian population because of high susceptibility
towards infectious diseases in the world. Procalcitonin (PCT) is a
well-studied marker in foreign countries but needs to be established
in Indian population. In the last few years, importance of Arginase
as a marker of immunity has also increased exceptionally, because
this enzyme is essentially involved in different inflammatory
processes. Keeping these facts in mind PCT and Arginase were
evaluated for their utility as markers to diagnose sepsis.
Aim: To evaluate Arginase and PCT as biomarkers for early
diagnosis of sepsis.
Materials and Methods: Hundred adult patients (age >18
years) with Systemic Inflammatory Response Syndrome (SIRS)
attending BJ Medical College which is affiliated with Sassoon
General Hospital (Pune, Maharashtra, India) during May 2012-
July 2015 were incorporated in the study. Age n sex-matched of
100 samples healthy controls were also collected. Arginase was
estimated by Roman and Ray method and PCT by Enzyme-linked
Immunosorbent Assay Kit (ELISA) method. Unpaired t-test was
done to compare the mean biomarker levels between the cases
and controls. The Area Under Curve (AUC) was calculated using
Receiver Operating Curve (ROC). All the data analysis was set at
95% Confidence Interval (CI) and value p<0.05 are statistically
significant. Data analysis was done using Statistical Package
for Social Sciences (SPSS) version 21.0.
Results: In the study, a significant increase was observed in
the levels of arginase (p<0.01) and PCT (p<0.01) in cases as
compared to controls. ROC curves were plotted to find out the
cut-offs of arginase (4.6 IU/L) and PCT (0.04 ng/mL) to check
the diagnostic efficacy of both the biomarkers.
Conclusion: Serum PCT and arginase offers a high level of
accuracy than other currently available tests and hence can be
helpful in the management of sepsis. In addition to this apart
from high sensitivity and specificity arginase estimation is cost
effective as compared to PCT. |
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| ISSN: | 2249-782X 0973-709X |