Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis
ABSTRACT Introduction: Sepsis remains a major cause of death in critically ill patients in Indian population because of high susceptibility towards infectious diseases in the world. Procalcitonin (PCT) is a well-studied marker in foreign countries but needs to be established in Indian populatio...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2020-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/14053/45261_CE[Ra1]_F(SL)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
| _version_ | 1818387234307440640 |
|---|---|
| author | Shruti Rahul Mulgund Subodhini Anant Abhang |
| author_facet | Shruti Rahul Mulgund Subodhini Anant Abhang |
| author_sort | Shruti Rahul Mulgund |
| collection | DOAJ |
| description | ABSTRACT
Introduction: Sepsis remains a major cause of death in critically
ill patients in Indian population because of high susceptibility
towards infectious diseases in the world. Procalcitonin (PCT) is a
well-studied marker in foreign countries but needs to be established
in Indian population. In the last few years, importance of Arginase
as a marker of immunity has also increased exceptionally, because
this enzyme is essentially involved in different inflammatory
processes. Keeping these facts in mind PCT and Arginase were
evaluated for their utility as markers to diagnose sepsis.
Aim: To evaluate Arginase and PCT as biomarkers for early
diagnosis of sepsis.
Materials and Methods: Hundred adult patients (age >18
years) with Systemic Inflammatory Response Syndrome (SIRS)
attending BJ Medical College which is affiliated with Sassoon
General Hospital (Pune, Maharashtra, India) during May 2012-
July 2015 were incorporated in the study. Age n sex-matched of
100 samples healthy controls were also collected. Arginase was
estimated by Roman and Ray method and PCT by Enzyme-linked
Immunosorbent Assay Kit (ELISA) method. Unpaired t-test was
done to compare the mean biomarker levels between the cases
and controls. The Area Under Curve (AUC) was calculated using
Receiver Operating Curve (ROC). All the data analysis was set at
95% Confidence Interval (CI) and value p<0.05 are statistically
significant. Data analysis was done using Statistical Package
for Social Sciences (SPSS) version 21.0.
Results: In the study, a significant increase was observed in
the levels of arginase (p<0.01) and PCT (p<0.01) in cases as
compared to controls. ROC curves were plotted to find out the
cut-offs of arginase (4.6 IU/L) and PCT (0.04 ng/mL) to check
the diagnostic efficacy of both the biomarkers.
Conclusion: Serum PCT and arginase offers a high level of
accuracy than other currently available tests and hence can be
helpful in the management of sepsis. In addition to this apart
from high sensitivity and specificity arginase estimation is cost
effective as compared to PCT. |
| first_indexed | 2024-12-14T04:06:42Z |
| format | Article |
| id | doaj.art-3804b9a9d22843418452eb7d50e53552 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-14T04:06:42Z |
| publishDate | 2020-09-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-3804b9a9d22843418452eb7d50e535522022-12-21T23:17:48ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2020-09-01149BC14BC1710.7860/JCDR/2020/45261.14053Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of SepsisShruti Rahul Mulgund0Subodhini Anant Abhang1Lecturer, Department of Biochemistry, Sinhgad Dental College and Hospital, Pune, Maharashtra, India.Professor, Department of Biochemistry, Symbiosis Medical College for Women, Pune, Maharashtra, India.ABSTRACT Introduction: Sepsis remains a major cause of death in critically ill patients in Indian population because of high susceptibility towards infectious diseases in the world. Procalcitonin (PCT) is a well-studied marker in foreign countries but needs to be established in Indian population. In the last few years, importance of Arginase as a marker of immunity has also increased exceptionally, because this enzyme is essentially involved in different inflammatory processes. Keeping these facts in mind PCT and Arginase were evaluated for their utility as markers to diagnose sepsis. Aim: To evaluate Arginase and PCT as biomarkers for early diagnosis of sepsis. Materials and Methods: Hundred adult patients (age >18 years) with Systemic Inflammatory Response Syndrome (SIRS) attending BJ Medical College which is affiliated with Sassoon General Hospital (Pune, Maharashtra, India) during May 2012- July 2015 were incorporated in the study. Age n sex-matched of 100 samples healthy controls were also collected. Arginase was estimated by Roman and Ray method and PCT by Enzyme-linked Immunosorbent Assay Kit (ELISA) method. Unpaired t-test was done to compare the mean biomarker levels between the cases and controls. The Area Under Curve (AUC) was calculated using Receiver Operating Curve (ROC). All the data analysis was set at 95% Confidence Interval (CI) and value p<0.05 are statistically significant. Data analysis was done using Statistical Package for Social Sciences (SPSS) version 21.0. Results: In the study, a significant increase was observed in the levels of arginase (p<0.01) and PCT (p<0.01) in cases as compared to controls. ROC curves were plotted to find out the cut-offs of arginase (4.6 IU/L) and PCT (0.04 ng/mL) to check the diagnostic efficacy of both the biomarkers. Conclusion: Serum PCT and arginase offers a high level of accuracy than other currently available tests and hence can be helpful in the management of sepsis. In addition to this apart from high sensitivity and specificity arginase estimation is cost effective as compared to PCT.https://jcdr.net/articles/PDF/14053/45261_CE[Ra1]_F(SL)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdfinfectious diseaseinflammatory markerssystemic inflammatory response syndrome |
| spellingShingle | Shruti Rahul Mulgund Subodhini Anant Abhang Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis Journal of Clinical and Diagnostic Research infectious disease inflammatory markers systemic inflammatory response syndrome |
| title | Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis |
| title_full | Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis |
| title_fullStr | Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis |
| title_full_unstemmed | Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis |
| title_short | Arginase and Procalcitonin: Promising Biomarkers for Early Diagnosis of Sepsis |
| title_sort | arginase and procalcitonin promising biomarkers for early diagnosis of sepsis |
| topic | infectious disease inflammatory markers systemic inflammatory response syndrome |
| url | https://jcdr.net/articles/PDF/14053/45261_CE[Ra1]_F(SL)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
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