Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2
Dengue fever and dengue hemorrhagic fever are transmitted to humans by the Aedes aegypti and Aedes albopictus mosquitoes, with an observed 30-fold increase in global incidence the last 50 years. Despite the tremendous efforts invested anti-dengue virus research, no clinically approved vaccine or ant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Infectious Disease Reports |
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| Online Access: | https://www.pagepress.org/journals/index.php/idr/article/view/8744 |
| _version_ | 1818903591259209728 |
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| author | Teguh H. Sucipto Fahimah Martak |
| author_facet | Teguh H. Sucipto Fahimah Martak |
| author_sort | Teguh H. Sucipto |
| collection | DOAJ |
| description | Dengue fever and dengue hemorrhagic fever are transmitted to humans by the Aedes aegypti and Aedes albopictus mosquitoes, with an observed 30-fold increase in global incidence the last 50 years. Despite the tremendous efforts invested anti-dengue virus research, no clinically approved vaccine or antiviral chemotherapeutics are available for humans, and disease treatment is limited to supportive care. Over the years there has been a continuous interest in the chemistry of metal complexes with biological activity, including platinum complexes with antitumor activity and silver complexes with antimicrobial action. Aim of the project was to investigate [Cu(2,4,5-triphenyl-1H- imidazole)2 (H2O)2].Cl2 as antiviral compound that was further tested for inhibitory effect on the replication of dengue virus type 2 (DENV-2) in Vero cell. DENV-2 were infected in Vero cells and replication of virus was measured by Viral ToxGlo with selectivity index value (SI) and determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for com- pound. The standard curve between concentration of compound (μg/mL) and %viability of cells was analyzed by logarithmic cor- relation regression with regression equation. For infection rates, t-test was used to exam- ine the statistical significances among the concentrations of compound. P<0.05 was considered to be significant. The maximum inhibitory concentration (IC50) of [Cu(2,4,5- triphenyl-1H-imidazole)2 (H2O)2].Cl2 against DENV-2 was 98.62 μg/mL. The cytotoxic concentration (CC50) of compound against Vero cells was 300.36μg/mL. The SI values for [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 1.86. Based on selectivity index values, [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 can inhibit the growth of DENV- 2 and has low toxicity values for Vero cells. |
| first_indexed | 2024-12-19T20:53:58Z |
| format | Article |
| id | doaj.art-380810fc32904ab1ae1246be774ee22f |
| institution | Directory Open Access Journal |
| issn | 2036-7430 2036-7449 |
| language | English |
| last_indexed | 2024-12-19T20:53:58Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Infectious Disease Reports |
| spelling | doaj.art-380810fc32904ab1ae1246be774ee22f2022-12-21T20:06:02ZengMDPI AGInfectious Disease Reports2036-74302036-74492020-07-01121s10.4081/idr.2020.8744Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2Teguh H. Sucipto0Fahimah Martak1Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Jl. Mulyorejo, Surabaya, East JavaDepartment of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember, Jl. Raya ITS, Surabaya, East JavaDengue fever and dengue hemorrhagic fever are transmitted to humans by the Aedes aegypti and Aedes albopictus mosquitoes, with an observed 30-fold increase in global incidence the last 50 years. Despite the tremendous efforts invested anti-dengue virus research, no clinically approved vaccine or antiviral chemotherapeutics are available for humans, and disease treatment is limited to supportive care. Over the years there has been a continuous interest in the chemistry of metal complexes with biological activity, including platinum complexes with antitumor activity and silver complexes with antimicrobial action. Aim of the project was to investigate [Cu(2,4,5-triphenyl-1H- imidazole)2 (H2O)2].Cl2 as antiviral compound that was further tested for inhibitory effect on the replication of dengue virus type 2 (DENV-2) in Vero cell. DENV-2 were infected in Vero cells and replication of virus was measured by Viral ToxGlo with selectivity index value (SI) and determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for com- pound. The standard curve between concentration of compound (μg/mL) and %viability of cells was analyzed by logarithmic cor- relation regression with regression equation. For infection rates, t-test was used to exam- ine the statistical significances among the concentrations of compound. P<0.05 was considered to be significant. The maximum inhibitory concentration (IC50) of [Cu(2,4,5- triphenyl-1H-imidazole)2 (H2O)2].Cl2 against DENV-2 was 98.62 μg/mL. The cytotoxic concentration (CC50) of compound against Vero cells was 300.36μg/mL. The SI values for [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 1.86. Based on selectivity index values, [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 can inhibit the growth of DENV- 2 and has low toxicity values for Vero cells.https://www.pagepress.org/journals/index.php/idr/article/view/8744DENV-2Vero cells[Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2, inhibition |
| spellingShingle | Teguh H. Sucipto Fahimah Martak Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2 Infectious Disease Reports DENV-2 Vero cells [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2, inhibition |
| title | Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2 |
| title_full | Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2 |
| title_fullStr | Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2 |
| title_full_unstemmed | Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2 |
| title_short | Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2 |
| title_sort | inhibition of dengue virus serotype 2 in vero cells with cu 2 4 5 triphenyl 1h imidazole 2 h2o 2 cl2 |
| topic | DENV-2 Vero cells [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2, inhibition |
| url | https://www.pagepress.org/journals/index.php/idr/article/view/8744 |
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