Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats
Objective(s): Maternal high-fat diet (HFD) consumption has been linked to metabolic disorders and reproductive dysfunctions in offspring. Troxerutin (TRO) has anti-hyperlipidemic, anti-oxidant, and anti-inflammatory effects. This study examined the effects of TRO on apelin-13, its receptors mRNA and...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2019-06-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_12762_5d2c3f6bc77dc2c601ad73ea1f6f375a.pdf |
| _version_ | 1818147176515108864 |
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| author | Keivan Mehri Seyed Mehdi Banan Khojasteh Fereshteh Farajdokht Zohreh Zavvari Oskuye Hadi Ebrahimi Rogaye Diba Parvin Bayandor Maryam Hosseindoost Shirin Babri |
| author_facet | Keivan Mehri Seyed Mehdi Banan Khojasteh Fereshteh Farajdokht Zohreh Zavvari Oskuye Hadi Ebrahimi Rogaye Diba Parvin Bayandor Maryam Hosseindoost Shirin Babri |
| author_sort | Keivan Mehri |
| collection | DOAJ |
| description | Objective(s): Maternal high-fat diet (HFD) consumption has been linked to metabolic disorders and reproductive dysfunctions in offspring. Troxerutin (TRO) has anti-hyperlipidemic, anti-oxidant, and anti-inflammatory effects. This study examined the effects of TRO on apelin-13, its receptors mRNA and ovarian histological changes in the offspring of HFD fed rats. Materials and Methods: Female Wistar rats were randomly divided into control diet (CD) or HFD groups and received these diets for eight weeks. After mating, dams were assigned into four subgroups: CD, CD + TRO, HFD, and HFD + TRO, and received their respective diets until the end of lactation. Troxerutin (150 mg/kg/day) was gavaged in the CD + TRO and HFD + TRO groups during pregnancy. On the postnatal day (PND) 21 all female offspring were separated and fed CD until PND 90. On PND 90 animals were sacrificed and ovarian tissue samples were collected for further evaluation. Results: Results showed that HFD significantly decreased serum apelin-13 in the female offspring of the HFD dams, which was significantly reversed by TRO. Moreover, real-time polymerase chain reaction (PCR) analysis revealed that TRO treatment significantly decreased the ovarian mRNA expression of the apelin-13 receptor in the troxerutin-received offspring. Furthermore, histological examination revealed that TRO increased the number of atretic follicles in the ovaries of HFD+TRO offspring.Conclusion: Maternal high fat feeding compromises ovarian health including follicular growth and development in the adult offspring and troxerutin treatment improved negative effects of maternal HFD on the apelin-13 level and ovarian development of offspring. |
| first_indexed | 2024-12-11T12:31:05Z |
| format | Article |
| id | doaj.art-3808563472494dfca246b5869de9352b |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-11T12:31:05Z |
| publishDate | 2019-06-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-3808563472494dfca246b5869de9352b2022-12-22T01:07:14ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742019-06-0122663764210.22038/ijbms.2019.34158.812312762Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed ratsKeivan Mehri0Seyed Mehdi Banan Khojasteh1Fereshteh Farajdokht2Zohreh Zavvari Oskuye3Hadi Ebrahimi4Rogaye Diba5Parvin Bayandor6Maryam Hosseindoost7Shirin Babri8Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Animal Biology, Faculty of Natural Sciences, University of Tabriz, IranNeurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranObjective(s): Maternal high-fat diet (HFD) consumption has been linked to metabolic disorders and reproductive dysfunctions in offspring. Troxerutin (TRO) has anti-hyperlipidemic, anti-oxidant, and anti-inflammatory effects. This study examined the effects of TRO on apelin-13, its receptors mRNA and ovarian histological changes in the offspring of HFD fed rats. Materials and Methods: Female Wistar rats were randomly divided into control diet (CD) or HFD groups and received these diets for eight weeks. After mating, dams were assigned into four subgroups: CD, CD + TRO, HFD, and HFD + TRO, and received their respective diets until the end of lactation. Troxerutin (150 mg/kg/day) was gavaged in the CD + TRO and HFD + TRO groups during pregnancy. On the postnatal day (PND) 21 all female offspring were separated and fed CD until PND 90. On PND 90 animals were sacrificed and ovarian tissue samples were collected for further evaluation. Results: Results showed that HFD significantly decreased serum apelin-13 in the female offspring of the HFD dams, which was significantly reversed by TRO. Moreover, real-time polymerase chain reaction (PCR) analysis revealed that TRO treatment significantly decreased the ovarian mRNA expression of the apelin-13 receptor in the troxerutin-received offspring. Furthermore, histological examination revealed that TRO increased the number of atretic follicles in the ovaries of HFD+TRO offspring.Conclusion: Maternal high fat feeding compromises ovarian health including follicular growth and development in the adult offspring and troxerutin treatment improved negative effects of maternal HFD on the apelin-13 level and ovarian development of offspring.http://ijbms.mums.ac.ir/article_12762_5d2c3f6bc77dc2c601ad73ea1f6f375a.pdfApelin-13APJ receptorMaternal high-fat dietOvarian developmentTroxerutin |
| spellingShingle | Keivan Mehri Seyed Mehdi Banan Khojasteh Fereshteh Farajdokht Zohreh Zavvari Oskuye Hadi Ebrahimi Rogaye Diba Parvin Bayandor Maryam Hosseindoost Shirin Babri Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats Iranian Journal of Basic Medical Sciences Apelin-13 APJ receptor Maternal high-fat diet Ovarian development Troxerutin |
| title | Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats |
| title_full | Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats |
| title_fullStr | Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats |
| title_full_unstemmed | Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats |
| title_short | Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats |
| title_sort | effect of troxerutin on apelin 13 apelin receptors apj and ovarian histological changes in the offspring of high fat diet fed rats |
| topic | Apelin-13 APJ receptor Maternal high-fat diet Ovarian development Troxerutin |
| url | http://ijbms.mums.ac.ir/article_12762_5d2c3f6bc77dc2c601ad73ea1f6f375a.pdf |
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