High intensity interval training attenuates osteoarthritis-associated hyperalgesia in rats
Abstract High-intensity interval training (HIIT) is a physical therapy that may benefit patients with osteoarthritis (OA). Cacna2d1 is a calcium channel subunit protein that plays an important role in the activity of nerve cells. However, there is currently no evidence on HIIT relieving OA-associate...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-04-01
|
| Series: | Journal of Physiological Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12576-023-00866-4 |
| _version_ | 1797836535713759232 |
|---|---|
| author | Xinwei Wang Jiulong Song Peng Xia Qiang Lin Anliang Chen Kai Cheng Fane Kong Yi Shi Xueping Li |
| author_facet | Xinwei Wang Jiulong Song Peng Xia Qiang Lin Anliang Chen Kai Cheng Fane Kong Yi Shi Xueping Li |
| author_sort | Xinwei Wang |
| collection | DOAJ |
| description | Abstract High-intensity interval training (HIIT) is a physical therapy that may benefit patients with osteoarthritis (OA). Cacna2d1 is a calcium channel subunit protein that plays an important role in the activity of nerve cells. However, there is currently no evidence on HIIT relieving OA-associate hyperalgesia by decreased Cacna2d1. Our study established the OA rat models with intra-articular injection of monosodium iodoacetate (MIA). This experiment was divided into two stages. The first stage comprised three groups: the control, OA, and OA-HIIT groups. The second stage comprised two groups, including the AAV-C and AAV-shRNA-Cacna2d1 groups. OA rats were positioned at the L5–L6 segments, and 20 µl of AAV virus was injected intrathecally. The pain threshold, cartilage analysis, Cacna2d1, and pain neurotransmitters were measured and compared. The pain threshold was significantly lower in OA rats than in control rats from the first to the tenth week. Starting from the sixth week, OA-HIIT rats exhibited significantly increased pain thresholds. The expression of Cacna2d1 increased in OA rats. Moreover, the knockdown of Cacna2d1 significantly down-regulated the expression of c-Fos, SP, and Vglut2 in the posterior horn of the spinal cord. In conclusion, HIIT attenuates OA-associated hyperalgesia, which may be related to the down-regulation of Cacna2d1. |
| first_indexed | 2024-04-09T15:11:35Z |
| format | Article |
| id | doaj.art-380858c24bbe46a3a78f1a771f07539b |
| institution | Directory Open Access Journal |
| issn | 1880-6562 |
| language | English |
| last_indexed | 2024-04-09T15:11:35Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Physiological Sciences |
| spelling | doaj.art-380858c24bbe46a3a78f1a771f07539b2023-04-30T11:09:55ZengBMCJournal of Physiological Sciences1880-65622023-04-0173111010.1186/s12576-023-00866-4High intensity interval training attenuates osteoarthritis-associated hyperalgesia in ratsXinwei Wang0Jiulong Song1Peng Xia2Qiang Lin3Anliang Chen4Kai Cheng5Fane Kong6Yi Shi7Xueping Li8Department of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical UniversityAbstract High-intensity interval training (HIIT) is a physical therapy that may benefit patients with osteoarthritis (OA). Cacna2d1 is a calcium channel subunit protein that plays an important role in the activity of nerve cells. However, there is currently no evidence on HIIT relieving OA-associate hyperalgesia by decreased Cacna2d1. Our study established the OA rat models with intra-articular injection of monosodium iodoacetate (MIA). This experiment was divided into two stages. The first stage comprised three groups: the control, OA, and OA-HIIT groups. The second stage comprised two groups, including the AAV-C and AAV-shRNA-Cacna2d1 groups. OA rats were positioned at the L5–L6 segments, and 20 µl of AAV virus was injected intrathecally. The pain threshold, cartilage analysis, Cacna2d1, and pain neurotransmitters were measured and compared. The pain threshold was significantly lower in OA rats than in control rats from the first to the tenth week. Starting from the sixth week, OA-HIIT rats exhibited significantly increased pain thresholds. The expression of Cacna2d1 increased in OA rats. Moreover, the knockdown of Cacna2d1 significantly down-regulated the expression of c-Fos, SP, and Vglut2 in the posterior horn of the spinal cord. In conclusion, HIIT attenuates OA-associated hyperalgesia, which may be related to the down-regulation of Cacna2d1.https://doi.org/10.1186/s12576-023-00866-4High intensity interval trainingHyperalgesiaOsteoarthritisPain |
| spellingShingle | Xinwei Wang Jiulong Song Peng Xia Qiang Lin Anliang Chen Kai Cheng Fane Kong Yi Shi Xueping Li High intensity interval training attenuates osteoarthritis-associated hyperalgesia in rats Journal of Physiological Sciences High intensity interval training Hyperalgesia Osteoarthritis Pain |
| title | High intensity interval training attenuates osteoarthritis-associated hyperalgesia in rats |
| title_full | High intensity interval training attenuates osteoarthritis-associated hyperalgesia in rats |
| title_fullStr | High intensity interval training attenuates osteoarthritis-associated hyperalgesia in rats |
| title_full_unstemmed | High intensity interval training attenuates osteoarthritis-associated hyperalgesia in rats |
| title_short | High intensity interval training attenuates osteoarthritis-associated hyperalgesia in rats |
| title_sort | high intensity interval training attenuates osteoarthritis associated hyperalgesia in rats |
| topic | High intensity interval training Hyperalgesia Osteoarthritis Pain |
| url | https://doi.org/10.1186/s12576-023-00866-4 |
| work_keys_str_mv | AT xinweiwang highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT jiulongsong highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT pengxia highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT qianglin highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT anliangchen highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT kaicheng highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT fanekong highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT yishi highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats AT xuepingli highintensityintervaltrainingattenuatesosteoarthritisassociatedhyperalgesiainrats |