Activation of Drosophila hemocyte motility by the ecdysone hormone
Summary Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues tha...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2013-11-01
|
| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/2/12/1412 |
| _version_ | 1818900999034634240 |
|---|---|
| author | Christopher J. Sampson Unum Amin Juan-Pablo Couso |
| author_facet | Christopher J. Sampson Unum Amin Juan-Pablo Couso |
| author_sort | Christopher J. Sampson |
| collection | DOAJ |
| description | Summary
Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton. |
| first_indexed | 2024-12-19T20:12:46Z |
| format | Article |
| id | doaj.art-3808c0896c3e45b199e6221ad8858238 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-19T20:12:46Z |
| publishDate | 2013-11-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-3808c0896c3e45b199e6221ad88582382022-12-21T20:07:16ZengThe Company of BiologistsBiology Open2046-63902013-11-012121412142010.1242/bio.2013661920136619Activation of Drosophila hemocyte motility by the ecdysone hormoneChristopher J. SampsonUnum AminJuan-Pablo CousoSummary Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton.http://bio.biologists.org/content/2/12/1412Cell cultureCell migrationCytoskeleton dynamicsDrosophilaEcdysoneHemocytes |
| spellingShingle | Christopher J. Sampson Unum Amin Juan-Pablo Couso Activation of Drosophila hemocyte motility by the ecdysone hormone Biology Open Cell culture Cell migration Cytoskeleton dynamics Drosophila Ecdysone Hemocytes |
| title | Activation of Drosophila hemocyte motility by the ecdysone hormone |
| title_full | Activation of Drosophila hemocyte motility by the ecdysone hormone |
| title_fullStr | Activation of Drosophila hemocyte motility by the ecdysone hormone |
| title_full_unstemmed | Activation of Drosophila hemocyte motility by the ecdysone hormone |
| title_short | Activation of Drosophila hemocyte motility by the ecdysone hormone |
| title_sort | activation of drosophila hemocyte motility by the ecdysone hormone |
| topic | Cell culture Cell migration Cytoskeleton dynamics Drosophila Ecdysone Hemocytes |
| url | http://bio.biologists.org/content/2/12/1412 |
| work_keys_str_mv | AT christopherjsampson activationofdrosophilahemocytemotilitybytheecdysonehormone AT unumamin activationofdrosophilahemocytemotilitybytheecdysonehormone AT juanpablocouso activationofdrosophilahemocytemotilitybytheecdysonehormone |