Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective Study
Introduction: In the era of Direct Acting Antiviral (DAA), elimination of HCV is possible but persistence in extra hepatic sites is hindrance. Detection of HCV has been reported in platelets, however its impact on DAA therapy not been studied. Aim: Detection and quantification of HCV RNA from th...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2018-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/12336/36491_CE[Ra]_F(P)_PF1(AGAK)_PFA(AK)_PB(AG_SL)_PN(SL).pdf |
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| author | Pradheer Gupta Manish Chandra Choudhary Meenu Bajpai Ekta Gupta |
| author_facet | Pradheer Gupta Manish Chandra Choudhary Meenu Bajpai Ekta Gupta |
| author_sort | Pradheer Gupta |
| collection | DOAJ |
| description | Introduction: In the era of Direct Acting Antiviral (DAA),
elimination of HCV is possible but persistence in extra hepatic
sites is hindrance. Detection of HCV has been reported in
platelets, however its impact on DAA therapy not been studied.
Aim: Detection and quantification of HCV RNA from the platelets
and to compare with parallel plasma levels.
Materials and Methods: Whole blood collected from 62 chronic
HCV infected patients. HCV RNA quantitation was done from
paired plasma and platelet pool at baseline using commercial
real time PCR assay (Abbott).
Correlation calculated by Bland-Altman analysis. Bivariate
association and p-value calculated using chi-square tests.
Results: A total of 45 patients were viremic while 17 were nonviremic. Median plasma viral load was log105.7 (IQR: log103.55-
6.7) IU/mL, median platelet viral load was log104.13 (IQR: log100-
5.5) IU/mL. Genotype (GT) 3 (66.6%) was commonest followed
by GT 1 (33.3%). Sustained Virologic Response (SVR) was
attained in 39/45 patients (92.8%) patients while 3/45 patients
(6.67%) were non-responders. Viral load at baseline in platelet
pool was higher in non-responders when compared to those
achieved SVR (median log104.46 vs. log103.27, p=0.456).
Conclusion: HCV-RNA was efficiently detected in platelets.
Persistence in platelets could be one of the factors associated
with treatment failure. |
| first_indexed | 2024-12-12T13:14:09Z |
| format | Article |
| id | doaj.art-3811872ea1b447ce8028042d4834126f |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-12T13:14:09Z |
| publishDate | 2018-12-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-3811872ea1b447ce8028042d4834126f2022-12-22T00:23:27ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2018-12-011212DM01DM0310.7860/JCDR/2018/36491.12336Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective StudyPradheer Gupta0Manish Chandra Choudhary1Meenu Bajpai2Ekta Gupta3Senior Resident, Department of Clinical Virology, Institute of Liver and Biliary Sciences, Delhi, India.Senior Research Fellow, Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, Delhi, India.Additional Professor, Department of Transfusion Medicine, Institute of Liver and Biliary Sciences, Delhi, India.Additional Professor, Department of Clinical Virology, Institute of Liver and Biliary Sciences, Delhi, India.Introduction: In the era of Direct Acting Antiviral (DAA), elimination of HCV is possible but persistence in extra hepatic sites is hindrance. Detection of HCV has been reported in platelets, however its impact on DAA therapy not been studied. Aim: Detection and quantification of HCV RNA from the platelets and to compare with parallel plasma levels. Materials and Methods: Whole blood collected from 62 chronic HCV infected patients. HCV RNA quantitation was done from paired plasma and platelet pool at baseline using commercial real time PCR assay (Abbott). Correlation calculated by Bland-Altman analysis. Bivariate association and p-value calculated using chi-square tests. Results: A total of 45 patients were viremic while 17 were nonviremic. Median plasma viral load was log105.7 (IQR: log103.55- 6.7) IU/mL, median platelet viral load was log104.13 (IQR: log100- 5.5) IU/mL. Genotype (GT) 3 (66.6%) was commonest followed by GT 1 (33.3%). Sustained Virologic Response (SVR) was attained in 39/45 patients (92.8%) patients while 3/45 patients (6.67%) were non-responders. Viral load at baseline in platelet pool was higher in non-responders when compared to those achieved SVR (median log104.46 vs. log103.27, p=0.456). Conclusion: HCV-RNA was efficiently detected in platelets. Persistence in platelets could be one of the factors associated with treatment failure.https://jcdr.net/articles/PDF/12336/36491_CE[Ra]_F(P)_PF1(AGAK)_PFA(AK)_PB(AG_SL)_PN(SL).pdfdirect acting antiviralsreal time polymerase chain reactionsustained virologic response |
| spellingShingle | Pradheer Gupta Manish Chandra Choudhary Meenu Bajpai Ekta Gupta Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective Study Journal of Clinical and Diagnostic Research direct acting antivirals real time polymerase chain reaction sustained virologic response |
| title | Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective Study |
| title_full | Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective Study |
| title_fullStr | Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective Study |
| title_full_unstemmed | Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective Study |
| title_short | Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective Study |
| title_sort | platelets as an extra hepatic reservoir of hepatitis c virus rna a prospective study |
| topic | direct acting antivirals real time polymerase chain reaction sustained virologic response |
| url | https://jcdr.net/articles/PDF/12336/36491_CE[Ra]_F(P)_PF1(AGAK)_PFA(AK)_PB(AG_SL)_PN(SL).pdf |
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