Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes
Summary: T cells are able to recognize and kill pathogens that infect host cells, including bacteria, viruses, and tumor cells. Here, we present a protocol to detect T cell function and bacterial load in OVA-Listeria monocytogenes-infected mice. We provide a detailed description of the steps for det...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672300549X |
| _version_ | 1797671385552650240 |
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| author | Yudai Xu Zijian Wang Wen Gao Haoyun Wang Zhixian Wang Chenxuan Tian Guobing Chen |
| author_facet | Yudai Xu Zijian Wang Wen Gao Haoyun Wang Zhixian Wang Chenxuan Tian Guobing Chen |
| author_sort | Yudai Xu |
| collection | DOAJ |
| description | Summary: T cells are able to recognize and kill pathogens that infect host cells, including bacteria, viruses, and tumor cells. Here, we present a protocol to detect T cell function and bacterial load in OVA-Listeria monocytogenes-infected mice. We provide a detailed description of the steps for detecting OVA-specific CD8+ T cells and their cytokine expression levels in splenocytes using flow cytometry on day 7 after infecting mice with OVA-Listeria monocytogenes. Additionally, we describe the steps for detecting the OVA-Listeria monocytogenes load in the mouse liver.For complete details on the use and execution of this protocol, please refer to Chen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-11T21:14:45Z |
| format | Article |
| id | doaj.art-381441f761634fc8b99f1910f67eaae5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-11T21:14:45Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-381441f761634fc8b99f1910f67eaae52023-09-29T04:45:08ZengElsevierSTAR Protocols2666-16672023-12-0144102582Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenesYudai Xu0Zijian Wang1Wen Gao2Haoyun Wang3Zhixian Wang4Chenxuan Tian5Guobing Chen6Department of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou 510632, China; Guangdong-Hong Kong-Macau Great Bay Area Geroscience Joint Laboratory, Guangzhou 510632, ChinaDepartment of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou 510632, China; Guangdong-Hong Kong-Macau Great Bay Area Geroscience Joint Laboratory, Guangzhou 510632, ChinaGuangdong-Hong Kong-Macau Great Bay Area Geroscience Joint Laboratory, Guangzhou 510632, China; NHC Key Laboratory of Male Reproduction and Genetics, Guangzhou 510600, China; Department of Reproductive Medicine Center, Guangdong Provincial Reproductive Science Institute (Guangdong Provincial Fertility Hospital), Guangzhou 510600, ChinaDepartment of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou 510632, China; Guangdong-Hong Kong-Macau Great Bay Area Geroscience Joint Laboratory, Guangzhou 510632, ChinaDepartment of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou 510632, ChinaDepartment of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou 510632, ChinaDepartment of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou 510632, China; Guangdong-Hong Kong-Macau Great Bay Area Geroscience Joint Laboratory, Guangzhou 510632, China; Affiliated Huaqiao Hospital, Jinan University, Guangzhou 510630, China; Corresponding authorSummary: T cells are able to recognize and kill pathogens that infect host cells, including bacteria, viruses, and tumor cells. Here, we present a protocol to detect T cell function and bacterial load in OVA-Listeria monocytogenes-infected mice. We provide a detailed description of the steps for detecting OVA-specific CD8+ T cells and their cytokine expression levels in splenocytes using flow cytometry on day 7 after infecting mice with OVA-Listeria monocytogenes. Additionally, we describe the steps for detecting the OVA-Listeria monocytogenes load in the mouse liver.For complete details on the use and execution of this protocol, please refer to Chen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672300549XCell BiologySingle CellFlow Cytometry/Mass CytometryImmunologyMicrobiology |
| spellingShingle | Yudai Xu Zijian Wang Wen Gao Haoyun Wang Zhixian Wang Chenxuan Tian Guobing Chen Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes STAR Protocols Cell Biology Single Cell Flow Cytometry/Mass Cytometry Immunology Microbiology |
| title | Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes |
| title_full | Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes |
| title_fullStr | Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes |
| title_full_unstemmed | Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes |
| title_short | Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes |
| title_sort | detection of the cd8 t cell immune response in mice infected with ova listeria monocytogenes |
| topic | Cell Biology Single Cell Flow Cytometry/Mass Cytometry Immunology Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S266616672300549X |
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