A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions
<p>Abstract</p> <p>Background</p> <p>Calorie or dietary restriction extends life span in a wide range of organisms including the filamentous fungus <it>Podospora anserina</it>. Under dietary restricted conditions, <it>P. anserina </it>isolates ar...
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-04-01
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| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/8/9 |
| _version_ | 1811234292488994816 |
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| author | Hoekstra Rolf F Maas Marc FPM Debets Alfons JM |
| author_facet | Hoekstra Rolf F Maas Marc FPM Debets Alfons JM |
| author_sort | Hoekstra Rolf F |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Calorie or dietary restriction extends life span in a wide range of organisms including the filamentous fungus <it>Podospora anserina</it>. Under dietary restricted conditions, <it>P. anserina </it>isolates are several-fold longer lived. This is however not the case in isolates that carry one of the pAL2-1 homologous mitochondrial plasmids.</p> <p>Results</p> <p>We show that the pAL2-1 homologues act as 'insertional mutators' of the mitochondrial genome, which may explain their negative effect on life span extension. Sequencing revealed at least fourteen unique plasmid integration sites, of which twelve were located within the mitochondrial genome and two within copies of the plasmid itself. The plasmids were able to integrate in their entirety, via a non-homologous mode of recombination. Some of the integrated plasmid copies were truncated, which probably resulted from secondary, post-integrative, recombination processes. Integration sites were predominantly located within and surrounding the region containing the mitochondrial rDNA loci.</p> <p>Conclusion</p> <p>We propose a model for the mechanism of integration, based on innate modes of mtDNA recombination, and discuss its possible link with the plasmid's negative effect on dietary restriction mediated life span extension.</p> |
| first_indexed | 2024-04-12T11:34:06Z |
| format | Article |
| id | doaj.art-3814b863f81b463983b8b56ff5942403 |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-04-12T11:34:06Z |
| publishDate | 2007-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-3814b863f81b463983b8b56ff59424032022-12-22T03:34:53ZengBMCBMC Genetics1471-21562007-04-0181910.1186/1471-2156-8-9A mitochondrial mutator plasmid that causes senescence under dietary restricted conditionsHoekstra Rolf FMaas Marc FPMDebets Alfons JM<p>Abstract</p> <p>Background</p> <p>Calorie or dietary restriction extends life span in a wide range of organisms including the filamentous fungus <it>Podospora anserina</it>. Under dietary restricted conditions, <it>P. anserina </it>isolates are several-fold longer lived. This is however not the case in isolates that carry one of the pAL2-1 homologous mitochondrial plasmids.</p> <p>Results</p> <p>We show that the pAL2-1 homologues act as 'insertional mutators' of the mitochondrial genome, which may explain their negative effect on life span extension. Sequencing revealed at least fourteen unique plasmid integration sites, of which twelve were located within the mitochondrial genome and two within copies of the plasmid itself. The plasmids were able to integrate in their entirety, via a non-homologous mode of recombination. Some of the integrated plasmid copies were truncated, which probably resulted from secondary, post-integrative, recombination processes. Integration sites were predominantly located within and surrounding the region containing the mitochondrial rDNA loci.</p> <p>Conclusion</p> <p>We propose a model for the mechanism of integration, based on innate modes of mtDNA recombination, and discuss its possible link with the plasmid's negative effect on dietary restriction mediated life span extension.</p>http://www.biomedcentral.com/1471-2156/8/9 |
| spellingShingle | Hoekstra Rolf F Maas Marc FPM Debets Alfons JM A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions BMC Genetics |
| title | A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions |
| title_full | A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions |
| title_fullStr | A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions |
| title_full_unstemmed | A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions |
| title_short | A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions |
| title_sort | mitochondrial mutator plasmid that causes senescence under dietary restricted conditions |
| url | http://www.biomedcentral.com/1471-2156/8/9 |
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