Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani
Rhizoctonia solani is a widely distributed plant pathogen that can damage many crops. Here, we identified a novel mycovirus tentatively named Rhizoctonia solani partitivirus 433 (RsPV433) from an R. solani (AG-3) strain which caused tobacco target spot disease on flue-cured tobacco. RsPV433 was cons...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.978075/full |
| _version_ | 1797995438119321600 |
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| author | Xiangru Chen Zhaoyao Yu Yujia Sun Meipeng Yang Ning Jiang |
| author_facet | Xiangru Chen Zhaoyao Yu Yujia Sun Meipeng Yang Ning Jiang |
| author_sort | Xiangru Chen |
| collection | DOAJ |
| description | Rhizoctonia solani is a widely distributed plant pathogen that can damage many crops. Here, we identified a novel mycovirus tentatively named Rhizoctonia solani partitivirus 433 (RsPV433) from an R. solani (AG-3) strain which caused tobacco target spot disease on flue-cured tobacco. RsPV433 was consisted of two dsRNA segments with lengths of 2450 and 2273 bp, which encoded an RNA-dependent RNA polymerase and a coat protein, respectively. BLASTP results of RsPV433 showed that the closest relative of RsPV433 was Sarcosphaera coronaria partitivirus (QLC36830.1), with an identity of 60.85% on the RdRp amino sequence. Phylogenetic analysis indicated that RsPV433 belonged to the Betapartitivirus genus in the Partitiviridae family. The virus transmission experiment revealed that RsPV433 can be transmitted horizontally. We further tested the biological effect of RsPV433 on R. solani strains and found that the RsPV433-infected R. solani strain grew slower than the RsPV433-free strain on the PDA medium and RsPV433 seemed to have no obvious impact on the lesion inducing ability of R. solani. |
| first_indexed | 2024-04-11T10:00:19Z |
| format | Article |
| id | doaj.art-381542da3fa74101b73e1a6ae104e9e6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T10:00:19Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-381542da3fa74101b73e1a6ae104e9e62022-12-22T04:30:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.978075978075Molecular characterization of a novel partitivirus isolated from Rhizoctonia solaniXiangru Chen0Zhaoyao Yu1Yujia Sun2Meipeng Yang3Ning Jiang4Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang, ChinaKey Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang, ChinaKey Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang, ChinaKey Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang, ChinaAgronomic Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, ChinaRhizoctonia solani is a widely distributed plant pathogen that can damage many crops. Here, we identified a novel mycovirus tentatively named Rhizoctonia solani partitivirus 433 (RsPV433) from an R. solani (AG-3) strain which caused tobacco target spot disease on flue-cured tobacco. RsPV433 was consisted of two dsRNA segments with lengths of 2450 and 2273 bp, which encoded an RNA-dependent RNA polymerase and a coat protein, respectively. BLASTP results of RsPV433 showed that the closest relative of RsPV433 was Sarcosphaera coronaria partitivirus (QLC36830.1), with an identity of 60.85% on the RdRp amino sequence. Phylogenetic analysis indicated that RsPV433 belonged to the Betapartitivirus genus in the Partitiviridae family. The virus transmission experiment revealed that RsPV433 can be transmitted horizontally. We further tested the biological effect of RsPV433 on R. solani strains and found that the RsPV433-infected R. solani strain grew slower than the RsPV433-free strain on the PDA medium and RsPV433 seemed to have no obvious impact on the lesion inducing ability of R. solani.https://www.frontiersin.org/articles/10.3389/fmicb.2022.978075/fulldsRNA (double-stranded RNA)mycovirusPartitiviridaeRhizoctonia solanitobacco target spot disease |
| spellingShingle | Xiangru Chen Zhaoyao Yu Yujia Sun Meipeng Yang Ning Jiang Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani Frontiers in Microbiology dsRNA (double-stranded RNA) mycovirus Partitiviridae Rhizoctonia solani tobacco target spot disease |
| title | Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani |
| title_full | Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani |
| title_fullStr | Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani |
| title_full_unstemmed | Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani |
| title_short | Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani |
| title_sort | molecular characterization of a novel partitivirus isolated from rhizoctonia solani |
| topic | dsRNA (double-stranded RNA) mycovirus Partitiviridae Rhizoctonia solani tobacco target spot disease |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.978075/full |
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