Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea
The mycotoxin Ochratoxin A (OTA) is responsible for producing many effects on human and animal health. In this work, the evaluation of the presence of OTA in tea beverage samples consisted of extraction and preconcentration through the solidification of a floating organic drop (DLLME-SFO) combined w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-04-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021007660 |
| _version_ | 1819297375849545728 |
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| author | Mariel Cina María del Valle Ponce Luis Dante Martinez Soledad Cerutti |
| author_facet | Mariel Cina María del Valle Ponce Luis Dante Martinez Soledad Cerutti |
| author_sort | Mariel Cina |
| collection | DOAJ |
| description | The mycotoxin Ochratoxin A (OTA) is responsible for producing many effects on human and animal health. In this work, the evaluation of the presence of OTA in tea beverage samples consisted of extraction and preconcentration through the solidification of a floating organic drop (DLLME-SFO) combined with an additional octadecyl silane clean-up step. The obtained extract was analyzed by UHPLC-MS/MS. Interferences from the matrix were effectively reduced and, consequently, recovery increased from 43.18% ± 4.1%–96.02% ± 2.54%. The validation assays were carried out by external calibration and spiked samples, with satisfactory recoveries. An adequate dynamic calibration range was obtained over a concentration interval between 0.5 and 70 μg mL−1 OTA. Capabilities of detection and quantification were 0.5 and 1.4 μg mL−1. The obtained Green Certificate was compared with other techniques to establish the greenness profile of the procedure. Quantification of ochratoxin A levels in tea samples was performed. |
| first_indexed | 2024-12-24T05:13:01Z |
| format | Article |
| id | doaj.art-3818ecee05f34cdd8cd0edde3f9ad3d9 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-24T05:13:01Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-3818ecee05f34cdd8cd0edde3f9ad3d92022-12-21T17:13:37ZengElsevierHeliyon2405-84402021-04-0174e06663Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in teaMariel Cina0María del Valle Ponce1Luis Dante Martinez2Soledad Cerutti3Instituto de Química de San Luis (CCT-San Luis), Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Laboratorio de Espectrometría de Masas, Bloque III, Ejército de los Andes 950, San Luis, CP5700, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ, Buenos Aires, ArgentinaInstituto de Química de San Luis (CCT-San Luis), Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Laboratorio de Espectrometría de Masas, Bloque III, Ejército de los Andes 950, San Luis, CP5700, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ, Buenos Aires, ArgentinaInstituto de Química de San Luis (CCT-San Luis), Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Laboratorio de Espectrometría de Masas, Bloque III, Ejército de los Andes 950, San Luis, CP5700, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ, Buenos Aires, ArgentinaInstituto de Química de San Luis (CCT-San Luis), Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Laboratorio de Espectrometría de Masas, Bloque III, Ejército de los Andes 950, San Luis, CP5700, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina; Corresponding author.The mycotoxin Ochratoxin A (OTA) is responsible for producing many effects on human and animal health. In this work, the evaluation of the presence of OTA in tea beverage samples consisted of extraction and preconcentration through the solidification of a floating organic drop (DLLME-SFO) combined with an additional octadecyl silane clean-up step. The obtained extract was analyzed by UHPLC-MS/MS. Interferences from the matrix were effectively reduced and, consequently, recovery increased from 43.18% ± 4.1%–96.02% ± 2.54%. The validation assays were carried out by external calibration and spiked samples, with satisfactory recoveries. An adequate dynamic calibration range was obtained over a concentration interval between 0.5 and 70 μg mL−1 OTA. Capabilities of detection and quantification were 0.5 and 1.4 μg mL−1. The obtained Green Certificate was compared with other techniques to establish the greenness profile of the procedure. Quantification of ochratoxin A levels in tea samples was performed.http://www.sciencedirect.com/science/article/pii/S2405844021007660Ochratoxin ATeaDLLME-SFOUHPLC-MS/MSGreen certificate |
| spellingShingle | Mariel Cina María del Valle Ponce Luis Dante Martinez Soledad Cerutti Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea Heliyon Ochratoxin A Tea DLLME-SFO UHPLC-MS/MS Green certificate |
| title | Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea |
| title_full | Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea |
| title_fullStr | Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea |
| title_full_unstemmed | Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea |
| title_short | Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea |
| title_sort | development of a novel uhplc ms ms method for the determination of ochratoxin a in tea |
| topic | Ochratoxin A Tea DLLME-SFO UHPLC-MS/MS Green certificate |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021007660 |
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