Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field cases
Objectives: The study was undertaken with the objectives to perform seromonitoring of Peste des Petits Ruminants (PPR) antibodies in goats vaccinated with PPR vaccine and molecular character¬ization of PPR virus (PPRV) from field cases in Bangladesh. Materials and Methods: Seromonitoring work was c...
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| Format: | Article |
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Network for the Veterinarians of Bangladesh
2019-09-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=56938 |
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| author | Shamim Ahamed K. H. M. Nazmul Hussain Nazir Md. Abu Yousuf Md. Monowarul Islam Md. Yeasin Arafat Md. Ariful Islam Md. Muket Mahmud Md. Rafiqul Islam |
| author_facet | Shamim Ahamed K. H. M. Nazmul Hussain Nazir Md. Abu Yousuf Md. Monowarul Islam Md. Yeasin Arafat Md. Ariful Islam Md. Muket Mahmud Md. Rafiqul Islam |
| author_sort | Shamim Ahamed |
| collection | DOAJ |
| description | Objectives: The study was undertaken with the objectives to perform seromonitoring of Peste des Petits Ruminants (PPR) antibodies in goats vaccinated with PPR vaccine and molecular character¬ization of PPR virus (PPRV) from field cases in Bangladesh.
Materials and Methods: Seromonitoring work was conducted in Char Kalibari, Mymensingh Sadar, Mymensingh. For this, a total of 50 goats were randomly selected and were divided into two groups; vaccinated (Group A; n = 25) and non-vaccinated (Group B; n = 25). The goats of both groups were again sub-divided into four age groups; (i) 06 months (n = 5), (ii) 612 months (n = 5), (iii) 1224 months (n = 10), and (iv) >24 months (n = 5). Blood samples were collected on Day-0 and after 21 days of post-vaccination (DPV), and the sera were prepared. The sera were examined for the presence of antibodies against PPRV by competitive enzyme-linked immunosorbent assay. For molecular characterization, nasal swabs (n = 10) were collected from PPR infected goats in Jessore during PPR outbreak (February 2016). The causative agent, PPRV isolated from field cases were confirmed by N gene based on reverse transcription polymerase chain reaction (RT-PCR), followed by sequencing, phylogenetic analysis, and multiple sequence alignment analyses.
Results: In the case of seromonitoring, the results revealed that before vaccination (at Day-0), overall, 44% (n = 22/50) goats were seropositive for PPRV. In Group A, 48% (n = 12/25) goats were seropositive, but after 21 DPV, 96% (n = 24/25) goats become seropositive. On the other hand, in Group B, 40% (n = 10/25) and 16% (n = 04/25) seropositive goats found at Day-0 and after 21 DPV, respectively, indicating that the antibody titer was increasing after vaccination and decreasing in convalescent goats. Out of 10 nasal swab samples, 40% (n = 4/10) was confirmed by RT-PCR targeting nucleocapsid (N gene). Phylogenetically, our isolate (KY039156/PPRV/BDG/Jes/2016) was similar to the other strains of PPRV under lineage IV. However, there was a unique amino acid substitution, where glycine (G) was recorded in place of arginine (R). The strain is closely related with other Chinese or Indian strains. The nucleotide sequence homology by NCBI BLAST search of the isolated strain ranged from 95% to 99% with other strains circulating in Bangladesh.
Conclusion: The PPRV is prevailing in the Mymensingh and Jessore regions of Bangladesh. Effective control of PPR in goats may depend on vaccination with PPR vaccine. Molecular characterization of PPRV in Jessore reveals that the virus is differing from the strain prevalent in other regions of Bangladesh and the world. [J Adv Vet Anim Res 2019; 6(3.000): 416-424] |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-12-13T02:19:09Z |
| publishDate | 2019-09-01 |
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| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-381a7ba0439f426eb0cea5934d82fd282022-12-22T00:02:49ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102019-09-016341642410.5455/javar.2019.f36256938Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field casesShamim Ahamed0K. H. M. Nazmul Hussain Nazir1Md. Abu Yousuf2Md. Monowarul Islam3Md. Yeasin Arafat4Md. Ariful Islam5Md. Muket Mahmud6Md. Rafiqul Islam7Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Animal Health Research Division, Bangladesh Livestock Research Institute (BLRI) Savar, Dhaka, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Livestock Division, Bangladesh Agricultural Research Council, Dhaka, Bangladesh.Objectives: The study was undertaken with the objectives to perform seromonitoring of Peste des Petits Ruminants (PPR) antibodies in goats vaccinated with PPR vaccine and molecular character¬ization of PPR virus (PPRV) from field cases in Bangladesh. Materials and Methods: Seromonitoring work was conducted in Char Kalibari, Mymensingh Sadar, Mymensingh. For this, a total of 50 goats were randomly selected and were divided into two groups; vaccinated (Group A; n = 25) and non-vaccinated (Group B; n = 25). The goats of both groups were again sub-divided into four age groups; (i) 06 months (n = 5), (ii) 612 months (n = 5), (iii) 1224 months (n = 10), and (iv) >24 months (n = 5). Blood samples were collected on Day-0 and after 21 days of post-vaccination (DPV), and the sera were prepared. The sera were examined for the presence of antibodies against PPRV by competitive enzyme-linked immunosorbent assay. For molecular characterization, nasal swabs (n = 10) were collected from PPR infected goats in Jessore during PPR outbreak (February 2016). The causative agent, PPRV isolated from field cases were confirmed by N gene based on reverse transcription polymerase chain reaction (RT-PCR), followed by sequencing, phylogenetic analysis, and multiple sequence alignment analyses. Results: In the case of seromonitoring, the results revealed that before vaccination (at Day-0), overall, 44% (n = 22/50) goats were seropositive for PPRV. In Group A, 48% (n = 12/25) goats were seropositive, but after 21 DPV, 96% (n = 24/25) goats become seropositive. On the other hand, in Group B, 40% (n = 10/25) and 16% (n = 04/25) seropositive goats found at Day-0 and after 21 DPV, respectively, indicating that the antibody titer was increasing after vaccination and decreasing in convalescent goats. Out of 10 nasal swab samples, 40% (n = 4/10) was confirmed by RT-PCR targeting nucleocapsid (N gene). Phylogenetically, our isolate (KY039156/PPRV/BDG/Jes/2016) was similar to the other strains of PPRV under lineage IV. However, there was a unique amino acid substitution, where glycine (G) was recorded in place of arginine (R). The strain is closely related with other Chinese or Indian strains. The nucleotide sequence homology by NCBI BLAST search of the isolated strain ranged from 95% to 99% with other strains circulating in Bangladesh. Conclusion: The PPRV is prevailing in the Mymensingh and Jessore regions of Bangladesh. Effective control of PPR in goats may depend on vaccination with PPR vaccine. Molecular characterization of PPRV in Jessore reveals that the virus is differing from the strain prevalent in other regions of Bangladesh and the world. [J Adv Vet Anim Res 2019; 6(3.000): 416-424]http://www.ejmanager.com/fulltextpdf.php?mno=56938PPR virus; cELISA; Jessore; RT-PCR; Mymensingh; phylogenetic analysis; sequencing |
| spellingShingle | Shamim Ahamed K. H. M. Nazmul Hussain Nazir Md. Abu Yousuf Md. Monowarul Islam Md. Yeasin Arafat Md. Ariful Islam Md. Muket Mahmud Md. Rafiqul Islam Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field cases Journal of Advanced Veterinary and Animal Research PPR virus; cELISA; Jessore; RT-PCR; Mymensingh; phylogenetic analysis; sequencing |
| title | Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field cases |
| title_full | Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field cases |
| title_fullStr | Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field cases |
| title_full_unstemmed | Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field cases |
| title_short | Seromonitoring of Peste des Petits Ruminants in goats and molecular characterization of PPR virus from field cases |
| title_sort | seromonitoring of peste des petits ruminants in goats and molecular characterization of ppr virus from field cases |
| topic | PPR virus; cELISA; Jessore; RT-PCR; Mymensingh; phylogenetic analysis; sequencing |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=56938 |
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