Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO)
Sub chronic exposure to lead in rats slows gastric emptying, but little is known about the effects of lead on gastric secretion. This study was designed to investigate the effects of lead on gastric acid secretion and its possible mechanisms in rats. Lead acetate was dissolved in drinking water in a...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2011-01-01
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| Series: | Acta Medica Iranica |
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| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/3681 |
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| author | Zakieh Vahedian Fatemeh Nabavizadeh Mansoor Keshavarz Jalal Vahedian Fatemeh Mirershadi |
| author_facet | Zakieh Vahedian Fatemeh Nabavizadeh Mansoor Keshavarz Jalal Vahedian Fatemeh Mirershadi |
| author_sort | Zakieh Vahedian |
| collection | DOAJ |
| description | Sub chronic exposure to lead in rats slows gastric emptying, but little is known about the effects of lead on gastric secretion. This study was designed to investigate the effects of lead on gastric acid secretion and its possible mechanisms in rats. Lead acetate was dissolved in drinking water in a concentration of 1%. Sodium acetate-containing water with a molar concentration similar to lead was also prepared. We had nine groups of animals (n=8); four of them were exposed to lead for 1, 2, 3, and 4 weeks (Pb1, Pb2, Pb3 and Pb4 groups, respectively). Sodium acetate solution was given to another four groups for 1, 2, 3, and 4 weeks (Na1, Na2, Na3 and Na4 groups, respectively). Gastric secretion was collected by washout technique and its acid output was measured in the basal (Basal Acid Output, BAO), vogotomy (Vagotomized Acid Output, VAO), and vagally stimulated (Vagally Stimulated Acid Output, VSAO) states using titrator instrument. Nitric oxide (NO) metabolite of gastric tissue was determined by Griess micro assay method to evaluate the possible mechanism of lead effect on gastric secretion. VSAO was significantly less in Pb1 and Pb2 groups than Na1 and Na2 ones respectively (1.75 ± 0.17, 2.10 ± 0.30 vs. 5.79 ± 0.20, 6.18 ± 0.27 µmol/15min) (P=0.001, P=0.001). BAO was significantly more in Pb3 and Pb4 groups than Na3 and Na4 ones respectively (2.77 ± 0.37, 2.80 ± 0.31 vs. 1.73 ± 0.16, 1.79 ± 0.34 µmol/15min) (P=0.01, P=0.02), but it was the same after vagotomy. VSAO was more in Pb3 and Pb4 groups than their Na counterparts (P=0.001, P=0.0001). NO metabolite of gastric tissue was more in all Pb groups in comparison to their Na counterparts (P=0.0001). In this study, it seems that lead exposure, via NO mechanism, has different effects on acid secretion. Nitric oxide in small and large amounts decrease and increase gastric acid secretion, respectively. |
| first_indexed | 2024-04-12T20:22:21Z |
| format | Article |
| id | doaj.art-381d29d4b1304319b47d0ce3669ac9ea |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-04-12T20:22:21Z |
| publishDate | 2011-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-381d29d4b1304319b47d0ce3669ac9ea2022-12-22T03:17:57ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942011-01-01491Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO)Zakieh Vahedian0Fatemeh Nabavizadeh1Mansoor Keshavarz2Jalal Vahedian3Fatemeh Mirershadi4School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of General Surgery, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Master Science of Physiology, Ardabil Azad University, Ardabil, Iran.Sub chronic exposure to lead in rats slows gastric emptying, but little is known about the effects of lead on gastric secretion. This study was designed to investigate the effects of lead on gastric acid secretion and its possible mechanisms in rats. Lead acetate was dissolved in drinking water in a concentration of 1%. Sodium acetate-containing water with a molar concentration similar to lead was also prepared. We had nine groups of animals (n=8); four of them were exposed to lead for 1, 2, 3, and 4 weeks (Pb1, Pb2, Pb3 and Pb4 groups, respectively). Sodium acetate solution was given to another four groups for 1, 2, 3, and 4 weeks (Na1, Na2, Na3 and Na4 groups, respectively). Gastric secretion was collected by washout technique and its acid output was measured in the basal (Basal Acid Output, BAO), vogotomy (Vagotomized Acid Output, VAO), and vagally stimulated (Vagally Stimulated Acid Output, VSAO) states using titrator instrument. Nitric oxide (NO) metabolite of gastric tissue was determined by Griess micro assay method to evaluate the possible mechanism of lead effect on gastric secretion. VSAO was significantly less in Pb1 and Pb2 groups than Na1 and Na2 ones respectively (1.75 ± 0.17, 2.10 ± 0.30 vs. 5.79 ± 0.20, 6.18 ± 0.27 µmol/15min) (P=0.001, P=0.001). BAO was significantly more in Pb3 and Pb4 groups than Na3 and Na4 ones respectively (2.77 ± 0.37, 2.80 ± 0.31 vs. 1.73 ± 0.16, 1.79 ± 0.34 µmol/15min) (P=0.01, P=0.02), but it was the same after vagotomy. VSAO was more in Pb3 and Pb4 groups than their Na counterparts (P=0.001, P=0.0001). NO metabolite of gastric tissue was more in all Pb groups in comparison to their Na counterparts (P=0.0001). In this study, it seems that lead exposure, via NO mechanism, has different effects on acid secretion. Nitric oxide in small and large amounts decrease and increase gastric acid secretion, respectively.https://acta.tums.ac.ir/index.php/acta/article/view/3681LeadNitric oxideGastric acidRats |
| spellingShingle | Zakieh Vahedian Fatemeh Nabavizadeh Mansoor Keshavarz Jalal Vahedian Fatemeh Mirershadi Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO) Acta Medica Iranica Lead Nitric oxide Gastric acid Rats |
| title | Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO) |
| title_full | Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO) |
| title_fullStr | Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO) |
| title_full_unstemmed | Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO) |
| title_short | Lead Exposure Changes Gastric Acid Secretion in Rat: Role of Nitric Oxide (NO) |
| title_sort | lead exposure changes gastric acid secretion in rat role of nitric oxide no |
| topic | Lead Nitric oxide Gastric acid Rats |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/3681 |
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