The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homes
Background Residents in long-term care homes, who tend to be of advanced age and frail, are at increased risk of respiratory infections. The respiratory microbiota is known to change with age, but whether these changes contribute to the risk of infection is not known. Our goal was to determine how t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
European Respiratory Society
2023-09-01
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| Series: | ERJ Open Research |
| Online Access: | http://openres.ersjournals.com/content/9/5/00212-2023.full |
| _version_ | 1797629331024904192 |
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| author | Dawn M.E. Bowdish Laura Rossi Mark Loeb Jennie Johnstone Louis P. Schenck Michelle Fontes Michael G. Surette Fiona J. Whelan |
| author_facet | Dawn M.E. Bowdish Laura Rossi Mark Loeb Jennie Johnstone Louis P. Schenck Michelle Fontes Michael G. Surette Fiona J. Whelan |
| author_sort | Dawn M.E. Bowdish |
| collection | DOAJ |
| description | Background
Residents in long-term care homes, who tend to be of advanced age and frail, are at increased risk of respiratory infections. The respiratory microbiota is known to change with age, but whether these changes contribute to the risk of infection is not known. Our goal was to determine how the nasal microbiota of frail older adults changes during symptoms of influenza-like illness (ILI) and how this may be impacted by enrolment in a placebo-controlled trial testing the feasibility of administering a Lactobacillus rhamnosus GG probiotic to prevent respiratory infection (2014–2017).
Methods
The microbiome of the nasal (mid-turbinate) of 150 residents of long-term care homes was interrogated using 16S rRNA gene sequencing.
Results
We identified a diverse and individualised microbiota which could be separated into nine distinct clusters based on Bray–Curtis distances. Samples collected during symptoms of ILI differed statistically from those collected pre- and post-cold and influenza season, and we observed decreased temporal stability (as measured by movement between clusters) in individuals who experienced ILI compared to those who did not.
Conclusions
The use of probiotics decreased ILI-induced changes to the microbiota; however, it is not clear whether this decrease is sufficient to prevent respiratory illness. |
| first_indexed | 2024-03-11T10:51:51Z |
| format | Article |
| id | doaj.art-381dac665cf5466ca2de5934064c097e |
| institution | Directory Open Access Journal |
| issn | 2312-0541 |
| language | English |
| last_indexed | 2024-03-11T10:51:51Z |
| publishDate | 2023-09-01 |
| publisher | European Respiratory Society |
| record_format | Article |
| series | ERJ Open Research |
| spelling | doaj.art-381dac665cf5466ca2de5934064c097e2023-11-13T14:27:50ZengEuropean Respiratory SocietyERJ Open Research2312-05412023-09-019510.1183/23120541.00212-202300212-2023The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homesDawn M.E. Bowdish0Laura Rossi1Mark Loeb2Jennie Johnstone3Louis P. Schenck4Michelle Fontes5Michael G. Surette6Fiona J. Whelan7 Department of Medicine, McMaster University, Hamilton, ON, Canada Department of Medicine, McMaster University, Hamilton, ON, Canada M.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, Canada Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada Department of Medicine, McMaster University, Hamilton, ON, Canada Department of Medicine, McMaster University, Hamilton, ON, Canada School of Life Sciences, University of Nottingham, Nottingham, UK Background Residents in long-term care homes, who tend to be of advanced age and frail, are at increased risk of respiratory infections. The respiratory microbiota is known to change with age, but whether these changes contribute to the risk of infection is not known. Our goal was to determine how the nasal microbiota of frail older adults changes during symptoms of influenza-like illness (ILI) and how this may be impacted by enrolment in a placebo-controlled trial testing the feasibility of administering a Lactobacillus rhamnosus GG probiotic to prevent respiratory infection (2014–2017). Methods The microbiome of the nasal (mid-turbinate) of 150 residents of long-term care homes was interrogated using 16S rRNA gene sequencing. Results We identified a diverse and individualised microbiota which could be separated into nine distinct clusters based on Bray–Curtis distances. Samples collected during symptoms of ILI differed statistically from those collected pre- and post-cold and influenza season, and we observed decreased temporal stability (as measured by movement between clusters) in individuals who experienced ILI compared to those who did not. Conclusions The use of probiotics decreased ILI-induced changes to the microbiota; however, it is not clear whether this decrease is sufficient to prevent respiratory illness.http://openres.ersjournals.com/content/9/5/00212-2023.full |
| spellingShingle | Dawn M.E. Bowdish Laura Rossi Mark Loeb Jennie Johnstone Louis P. Schenck Michelle Fontes Michael G. Surette Fiona J. Whelan The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homes ERJ Open Research |
| title | The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homes |
| title_full | The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homes |
| title_fullStr | The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homes |
| title_full_unstemmed | The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homes |
| title_short | The impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long-term care homes |
| title_sort | impact of respiratory infections and probiotic use on the nasal microbiota of frail residents in long term care homes |
| url | http://openres.ersjournals.com/content/9/5/00212-2023.full |
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