Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials
Abstract Background Zoonotic livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widely spread in pig herds in many countries. However, the knowledge regarding the survival of LA-MRSA in the pig farm environment is currently limited. The aim of this study was to assess the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-03-01
|
| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13028-023-00676-z |
| _version_ | 1797863934446796800 |
|---|---|
| author | Krista Tuominen Sara Frosth Karl Pedersen Thomas Rosendal Susanna Sternberg Lewerin |
| author_facet | Krista Tuominen Sara Frosth Karl Pedersen Thomas Rosendal Susanna Sternberg Lewerin |
| author_sort | Krista Tuominen |
| collection | DOAJ |
| description | Abstract Background Zoonotic livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widely spread in pig herds in many countries. However, the knowledge regarding the survival of LA-MRSA in the pig farm environment is currently limited. The aim of this study was to assess the survival of LA-MRSA on different surface materials found in the farm environment. The study investigated the survival of two different LA-MRSA strains belonging to the clonal complex (CC) 398 on four different surfaces: stainless steel, polypropylene plastic, K30 concrete and commercial concrete disk coupons. The survival of the bacteria over time was determined by the viable count method and, where possible, fitting a model to the observed data by using nonlinear least squares method to calculate the half-life ( $${t}_{1/2}$$ t 1 / 2 ) for different strain and material combinations. Results The study showed that the half-life of the bacteria was longer on polypropylene plastic ( $${t}_{1/2}$$ t 1 / 2 =11.08–15.78 days) than on stainless steel ( $${t}_{1/2}$$ t 1 / 2 =2.45–7.83 days). On these materials, both LA-MRSA strains survived through the 14 week observation period. The bacterial decay was fastest on the concrete surfaces, where LA-MRSA became undetectable after 3–9 weeks. Conclusions The survival of LA-MRSA in the pig farm environment may be affected by different surface materials. A more frequent sampling protocol (< 7 days) is needed to determine the half-life on concrete surfaces. |
| first_indexed | 2024-04-09T22:44:38Z |
| format | Article |
| id | doaj.art-3820d9e01dd240cd81f17757622a7342 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-04-09T22:44:38Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-3820d9e01dd240cd81f17757622a73422023-03-22T11:57:14ZengBMCActa Veterinaria Scandinavica1751-01472023-03-016511810.1186/s13028-023-00676-zSurvival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materialsKrista Tuominen0Sara Frosth1Karl Pedersen2Thomas Rosendal3Susanna Sternberg Lewerin4Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural SciencesDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural SciencesDepartment of Animal Health and Antimicrobial Strategies, National Veterinary InstituteDepartment of Disease Control and Epidemiology, National Veterinary InstituteDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural SciencesAbstract Background Zoonotic livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widely spread in pig herds in many countries. However, the knowledge regarding the survival of LA-MRSA in the pig farm environment is currently limited. The aim of this study was to assess the survival of LA-MRSA on different surface materials found in the farm environment. The study investigated the survival of two different LA-MRSA strains belonging to the clonal complex (CC) 398 on four different surfaces: stainless steel, polypropylene plastic, K30 concrete and commercial concrete disk coupons. The survival of the bacteria over time was determined by the viable count method and, where possible, fitting a model to the observed data by using nonlinear least squares method to calculate the half-life ( $${t}_{1/2}$$ t 1 / 2 ) for different strain and material combinations. Results The study showed that the half-life of the bacteria was longer on polypropylene plastic ( $${t}_{1/2}$$ t 1 / 2 =11.08–15.78 days) than on stainless steel ( $${t}_{1/2}$$ t 1 / 2 =2.45–7.83 days). On these materials, both LA-MRSA strains survived through the 14 week observation period. The bacterial decay was fastest on the concrete surfaces, where LA-MRSA became undetectable after 3–9 weeks. Conclusions The survival of LA-MRSA in the pig farm environment may be affected by different surface materials. A more frequent sampling protocol (< 7 days) is needed to determine the half-life on concrete surfaces.https://doi.org/10.1186/s13028-023-00676-zBacterial decayConcreteEnvironmentHalf-lifeLA-MRSAPlastic |
| spellingShingle | Krista Tuominen Sara Frosth Karl Pedersen Thomas Rosendal Susanna Sternberg Lewerin Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials Acta Veterinaria Scandinavica Bacterial decay Concrete Environment Half-life LA-MRSA Plastic |
| title | Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials |
| title_full | Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials |
| title_fullStr | Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials |
| title_full_unstemmed | Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials |
| title_short | Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials |
| title_sort | survival of livestock associated methicillin resistant staphylococcus aureus cc398 on different surface materials |
| topic | Bacterial decay Concrete Environment Half-life LA-MRSA Plastic |
| url | https://doi.org/10.1186/s13028-023-00676-z |
| work_keys_str_mv | AT kristatuominen survivaloflivestockassociatedmethicillinresistantstaphylococcusaureuscc398ondifferentsurfacematerials AT sarafrosth survivaloflivestockassociatedmethicillinresistantstaphylococcusaureuscc398ondifferentsurfacematerials AT karlpedersen survivaloflivestockassociatedmethicillinresistantstaphylococcusaureuscc398ondifferentsurfacematerials AT thomasrosendal survivaloflivestockassociatedmethicillinresistantstaphylococcusaureuscc398ondifferentsurfacematerials AT susannasternberglewerin survivaloflivestockassociatedmethicillinresistantstaphylococcusaureuscc398ondifferentsurfacematerials |