A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells
Background/Purpose: In vitro neural cell-based models have been widely used to mimic the in vivo neural tissue environments and quantitatively understand the effects of pharmaceutical molecules on neural diseases. Recently, several biomimetic neural tissue models have been widely developed by using...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Journal of the Formosan Medical Association |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664619305236 |
| _version_ | 1819168658166906880 |
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| author | Hsiao-Cheng Tsai Jyh-Horng Wang Yun-An Chen Li-Kai Tsai Tai-Horng Young Yi-Chen Ethan Li |
| author_facet | Hsiao-Cheng Tsai Jyh-Horng Wang Yun-An Chen Li-Kai Tsai Tai-Horng Young Yi-Chen Ethan Li |
| author_sort | Hsiao-Cheng Tsai |
| collection | DOAJ |
| description | Background/Purpose: In vitro neural cell-based models have been widely used to mimic the in vivo neural tissue environments and quantitatively understand the effects of pharmaceutical molecules on neural diseases. Recently, several biomimetic neural tissue models have been widely developed by using biomaterials or surface modification. However, the complex protocols of material synthesis or surface modification lack an easy execution to fabricate the neuron favorite environment. Methods: In this study, we utilized a layer-by-layer technique as a surface modification method for regulating the behaviors of neural stem/precursor cells (NSPCs) on material surfaces. Polyelectrolyte multilayers (PEMs) via alternate deposition of poly (allylamine hydrochloride) (PAH) and poly (sodium-4-styrenesulfonate) (PSS) were used to culture NSPCs. After incubation for 7 days, the neuronal differentiation of NSPCs and synapse function of differentiated neurons were identified by immunocytochemistry for lineage specific markers. Results: Compared with the only PAH film, the PSS-ending film (neuron-rich model) was shown to significantly promote differentiation of NSPCs into neurons (more than 50%), form a neuronal network structure; and differentiated neurons exhibiting functional synaptic activity. Conclusion: This study shows that the PEMs provided an easily alternative approach to modify the surface properties; and might be a method to obtain a neuron-rich model for the biological/pharmaceutical applications. Keywords: Polyelectrolyte multilayer (PEM), Neural stem/precursor cells (NSPCs), Neuronal differentiation, Neurite outgrowth |
| first_indexed | 2024-12-22T19:07:06Z |
| format | Article |
| id | doaj.art-38213f91620a43479f41f5d68922f50a |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-22T19:07:06Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-38213f91620a43479f41f5d68922f50a2022-12-21T18:15:46ZengElsevierJournal of the Formosan Medical Association0929-66462020-01-011191430438A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cellsHsiao-Cheng Tsai0Jyh-Horng Wang1Yun-An Chen2Li-Kai Tsai3Tai-Horng Young4Yi-Chen Ethan Li5Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei 100, TaiwanDepartment of Orthopedic Surgery, National Taiwan University Hospital, Taipei, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, TaiwanDepartment of Neurology and Stroke Center, National Taiwan University Hospital and College of Medicine, Taipei, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan; Corresponding author. Fax: +886 2 23940049.Department of Chemical Engineering, Feng Chia University, Taichung, Taiwan; Corresponding author. Fax: +886 4 24510890.Background/Purpose: In vitro neural cell-based models have been widely used to mimic the in vivo neural tissue environments and quantitatively understand the effects of pharmaceutical molecules on neural diseases. Recently, several biomimetic neural tissue models have been widely developed by using biomaterials or surface modification. However, the complex protocols of material synthesis or surface modification lack an easy execution to fabricate the neuron favorite environment. Methods: In this study, we utilized a layer-by-layer technique as a surface modification method for regulating the behaviors of neural stem/precursor cells (NSPCs) on material surfaces. Polyelectrolyte multilayers (PEMs) via alternate deposition of poly (allylamine hydrochloride) (PAH) and poly (sodium-4-styrenesulfonate) (PSS) were used to culture NSPCs. After incubation for 7 days, the neuronal differentiation of NSPCs and synapse function of differentiated neurons were identified by immunocytochemistry for lineage specific markers. Results: Compared with the only PAH film, the PSS-ending film (neuron-rich model) was shown to significantly promote differentiation of NSPCs into neurons (more than 50%), form a neuronal network structure; and differentiated neurons exhibiting functional synaptic activity. Conclusion: This study shows that the PEMs provided an easily alternative approach to modify the surface properties; and might be a method to obtain a neuron-rich model for the biological/pharmaceutical applications. Keywords: Polyelectrolyte multilayer (PEM), Neural stem/precursor cells (NSPCs), Neuronal differentiation, Neurite outgrowthhttp://www.sciencedirect.com/science/article/pii/S0929664619305236 |
| spellingShingle | Hsiao-Cheng Tsai Jyh-Horng Wang Yun-An Chen Li-Kai Tsai Tai-Horng Young Yi-Chen Ethan Li A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells Journal of the Formosan Medical Association |
| title | A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells |
| title_full | A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells |
| title_fullStr | A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells |
| title_full_unstemmed | A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells |
| title_short | A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells |
| title_sort | self assembled layer by layer surface modification to fabricate the neuron rich model from neural stem precursor cells |
| url | http://www.sciencedirect.com/science/article/pii/S0929664619305236 |
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