A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device
Microfluidic systems have been widely utilized in high-throughput biology analysis, but the difficulties in liquid manipulation and cell cultivation limit its application. This work has developed a new digital microfluidic (DMF) system for on-demand droplet control. By adopting an extending-depth-of...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2023-05-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545822440060 |
| _version_ | 1797826269666082816 |
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| author | Qiushu Chen Qi Meng Yuzhe Liu Xiangan Long Yawei Kong Longfang Yao Liwen Chen Chuanyong Wu Kaiqin Chu Lan Mi Jiong Ma |
| author_facet | Qiushu Chen Qi Meng Yuzhe Liu Xiangan Long Yawei Kong Longfang Yao Liwen Chen Chuanyong Wu Kaiqin Chu Lan Mi Jiong Ma |
| author_sort | Qiushu Chen |
| collection | DOAJ |
| description | Microfluidic systems have been widely utilized in high-throughput biology analysis, but the difficulties in liquid manipulation and cell cultivation limit its application. This work has developed a new digital microfluidic (DMF) system for on-demand droplet control. By adopting an extending-depth-of-field (EDoF) phase modulator to the optical system, the entire depth of the microfluidic channel can be covered in one image without any refocusing process, ensuring that 95% of the particles in the droplet are captured within three shots together with shaking processes. With this system, suspension droplets are generated and droplets containing only one yeast cell can be recognized, then each single cell is cultured in the array of the chip. By observing their growth in cell numbers and the green fluorescence protein (GFP) production via fluorescence imaging, the single cell with the highest production can be identified. The results have proved the heterogeneity of yeast cells, and showed that the combined system can be applied for rapid single-cell sorting, cultivation, and analysis. |
| first_indexed | 2024-03-13T11:06:16Z |
| format | Article |
| id | doaj.art-382256a1f5d44d3d93711c39032eed05 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-03-13T11:06:16Z |
| publishDate | 2023-05-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-382256a1f5d44d3d93711c39032eed052023-05-16T06:39:07ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-05-01160310.1142/S1793545822440060A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field deviceQiushu Chen0Qi Meng1Yuzhe Liu2Xiangan Long3Yawei Kong4Longfang Yao5Liwen Chen6Chuanyong Wu7Kaiqin Chu8Lan Mi9Jiong Ma10Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230027, Anhui, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. ChinaInstitute of Biomedical Engineering and Technology, Academy for Engineering and Technology, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. ChinaShanghai Hengxin BioTechnology, Ltd., 1688 North Guo Quan Rd, Bldg A8, Rm 801, Shanghai 200438, P. R. ChinaDepartment of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230027, Anhui, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, P. R. ChinaMicrofluidic systems have been widely utilized in high-throughput biology analysis, but the difficulties in liquid manipulation and cell cultivation limit its application. This work has developed a new digital microfluidic (DMF) system for on-demand droplet control. By adopting an extending-depth-of-field (EDoF) phase modulator to the optical system, the entire depth of the microfluidic channel can be covered in one image without any refocusing process, ensuring that 95% of the particles in the droplet are captured within three shots together with shaking processes. With this system, suspension droplets are generated and droplets containing only one yeast cell can be recognized, then each single cell is cultured in the array of the chip. By observing their growth in cell numbers and the green fluorescence protein (GFP) production via fluorescence imaging, the single cell with the highest production can be identified. The results have proved the heterogeneity of yeast cells, and showed that the combined system can be applied for rapid single-cell sorting, cultivation, and analysis.https://www.worldscientific.com/doi/10.1142/S1793545822440060Single-cell analysisdigital microfluidic (DMF)extending-depth-of-field system |
| spellingShingle | Qiushu Chen Qi Meng Yuzhe Liu Xiangan Long Yawei Kong Longfang Yao Liwen Chen Chuanyong Wu Kaiqin Chu Lan Mi Jiong Ma A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device Journal of Innovative Optical Health Sciences Single-cell analysis digital microfluidic (DMF) extending-depth-of-field system |
| title | A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device |
| title_full | A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device |
| title_fullStr | A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device |
| title_full_unstemmed | A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device |
| title_short | A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device |
| title_sort | digital microfluidic single cell manipulation system optimized by extending depth of field device |
| topic | Single-cell analysis digital microfluidic (DMF) extending-depth-of-field system |
| url | https://www.worldscientific.com/doi/10.1142/S1793545822440060 |
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