Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash Bioconsolidation
Whey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP)...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/14/10/2470 |
| _version_ | 1797534648761319424 |
|---|---|
| author | Sandra Chaparro Hugo A. Rojas Gerardo Caicedo Gustavo Romanelli Antonio Pineda Rafael Luque José J. Martínez |
| author_facet | Sandra Chaparro Hugo A. Rojas Gerardo Caicedo Gustavo Romanelli Antonio Pineda Rafael Luque José J. Martínez |
| author_sort | Sandra Chaparro |
| collection | DOAJ |
| description | Whey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP). In this work, a native <i>Sporosarcina pasteurii</i> was isolated and then cultured by using different proportions of whey (W) in nutrient broth (NB). The solids were characterized by XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were also studied. Whey concentration was directly related to CaCO<sub>3</sub> production. Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All experiments showed calcite and vaterite fractions, where a whey increment in the media increased calcite content and decreased vaterite content, causing a decrease in crystal size. MICP improved compressive strength (CS) in sand and fly ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in biogenic CaCO<sub>3</sub> production. Material bioconsolidation depends on the CaCO<sub>3</sub> polymorph, thus fly ash was effectively bioconsolidated by crystallization of vaterite and sand by crystallization of calcite. |
| first_indexed | 2024-03-10T11:33:29Z |
| format | Article |
| id | doaj.art-382296e98d9c4a798847fda34a15c29a |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T11:33:29Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-382296e98d9c4a798847fda34a15c29a2023-11-21T19:04:39ZengMDPI AGMaterials1996-19442021-05-011410247010.3390/ma14102470Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash BioconsolidationSandra Chaparro0Hugo A. Rojas1Gerardo Caicedo2Gustavo Romanelli3Antonio Pineda4Rafael Luque5José J. Martínez6School of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological University of Colombia, 150001 Tunja, ColombiaSchool of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological University of Colombia, 150001 Tunja, ColombiaSchool of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological University of Colombia, 150001 Tunja, ColombiaResearch and Development Centre on Applied Sciences “Dr. Jorge Ronco” (CCT-La Plata-CONICET, CIC-PBA), National University of La Plata, 1900 La Plata, ArgentinaDepartamento de Química Orgánica, Universidad de Córdoba, Ctra NNal IV-A, Km 396, E-14014 Córdoba, SpainDepartamento de Química Orgánica, Universidad de Córdoba, Ctra NNal IV-A, Km 396, E-14014 Córdoba, SpainSchool of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological University of Colombia, 150001 Tunja, ColombiaWhey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP). In this work, a native <i>Sporosarcina pasteurii</i> was isolated and then cultured by using different proportions of whey (W) in nutrient broth (NB). The solids were characterized by XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were also studied. Whey concentration was directly related to CaCO<sub>3</sub> production. Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All experiments showed calcite and vaterite fractions, where a whey increment in the media increased calcite content and decreased vaterite content, causing a decrease in crystal size. MICP improved compressive strength (CS) in sand and fly ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in biogenic CaCO<sub>3</sub> production. Material bioconsolidation depends on the CaCO<sub>3</sub> polymorph, thus fly ash was effectively bioconsolidated by crystallization of vaterite and sand by crystallization of calcite.https://www.mdpi.com/1996-1944/14/10/2470biogenic CaCO<sub>3</sub>polymorphismureolytic bacteriawhey |
| spellingShingle | Sandra Chaparro Hugo A. Rojas Gerardo Caicedo Gustavo Romanelli Antonio Pineda Rafael Luque José J. Martínez Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash Bioconsolidation Materials biogenic CaCO<sub>3</sub> polymorphism ureolytic bacteria whey |
| title | Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash Bioconsolidation |
| title_full | Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash Bioconsolidation |
| title_fullStr | Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash Bioconsolidation |
| title_full_unstemmed | Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash Bioconsolidation |
| title_short | Whey as an Alternative Nutrient Medium for Growth of <i>Sporosarcina pasteurii</i> and Its Effect on CaCO<sub>3</sub> Polymorphism and Fly Ash Bioconsolidation |
| title_sort | whey as an alternative nutrient medium for growth of i sporosarcina pasteurii i and its effect on caco sub 3 sub polymorphism and fly ash bioconsolidation |
| topic | biogenic CaCO<sub>3</sub> polymorphism ureolytic bacteria whey |
| url | https://www.mdpi.com/1996-1944/14/10/2470 |
| work_keys_str_mv | AT sandrachaparro wheyasanalternativenutrientmediumforgrowthofisporosarcinapasteuriiianditseffectoncacosub3subpolymorphismandflyashbioconsolidation AT hugoarojas wheyasanalternativenutrientmediumforgrowthofisporosarcinapasteuriiianditseffectoncacosub3subpolymorphismandflyashbioconsolidation AT gerardocaicedo wheyasanalternativenutrientmediumforgrowthofisporosarcinapasteuriiianditseffectoncacosub3subpolymorphismandflyashbioconsolidation AT gustavoromanelli wheyasanalternativenutrientmediumforgrowthofisporosarcinapasteuriiianditseffectoncacosub3subpolymorphismandflyashbioconsolidation AT antoniopineda wheyasanalternativenutrientmediumforgrowthofisporosarcinapasteuriiianditseffectoncacosub3subpolymorphismandflyashbioconsolidation AT rafaelluque wheyasanalternativenutrientmediumforgrowthofisporosarcinapasteuriiianditseffectoncacosub3subpolymorphismandflyashbioconsolidation AT josejmartinez wheyasanalternativenutrientmediumforgrowthofisporosarcinapasteuriiianditseffectoncacosub3subpolymorphismandflyashbioconsolidation |