Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors
Abstract Purpose: To evaluate the expression of EGFR, KRAS genes, microRNAs-21 and 203 in colon and rectal cancer samples, correlated with their age at diagnosis, histological subtype, value of pretreatment CEA, TNM staging and clinical outcome. Methods: Expression of genes and microRNAs by real...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2017-03-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000300243&tlng=en |
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| author | Thais Inácio de Carvalho Paulo Cezar Novais Fermino Sanches Lizarte Neto Renata Danielle Sicchieri Marcella Suelma Torrecillas Rosa Camila Albuquerque Mello de Carvalho Daniela Pretti da Cunha Tirapelli Fernanda Maris Peria José Joaquim Ribeiro da Rocha Omar Féres |
| author_facet | Thais Inácio de Carvalho Paulo Cezar Novais Fermino Sanches Lizarte Neto Renata Danielle Sicchieri Marcella Suelma Torrecillas Rosa Camila Albuquerque Mello de Carvalho Daniela Pretti da Cunha Tirapelli Fernanda Maris Peria José Joaquim Ribeiro da Rocha Omar Féres |
| author_sort | Thais Inácio de Carvalho |
| collection | DOAJ |
| description | Abstract Purpose: To evaluate the expression of EGFR, KRAS genes, microRNAs-21 and 203 in colon and rectal cancer samples, correlated with their age at diagnosis, histological subtype, value of pretreatment CEA, TNM staging and clinical outcome. Methods: Expression of genes and microRNAs by real time PCR in tumor and non-tumor samples obtained from surgical treatment of 50 patients. Results: An increased expression of microRNAs-21 and 203 in tumor samples in relation to non-tumor samples was found. There was no statistically significant difference between the expression of these genes and microRNAs when compared to age at diagnosis and histological subtype. The EGFR gene showed higher expression in relation to the value of CEA diagnosis. The expression of microRNA-203 was progressively lower in relation to the TNM staging and was higher in the patient group in clinical remission. Conclusions: The therapy of colon and rectum tumors based on microRNAs remains under investigation reserving huge potential for future applications and clinical interventions in conjunction with existing therapies. We expect, based on the exposed data, to stimulate the development of new therapeutic possibilities, making the treatment of these tumors more effective. |
| first_indexed | 2024-12-22T02:29:25Z |
| format | Article |
| id | doaj.art-3822ecb0e2c543bca9a27956e1413025 |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-22T02:29:25Z |
| publishDate | 2017-03-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-3822ecb0e2c543bca9a27956e14130252022-12-21T18:41:55ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502017-03-0132324325010.1590/s0102-865020170030000009Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factorsThais Inácio de CarvalhoPaulo Cezar NovaisFermino Sanches Lizarte NetoRenata Danielle SicchieriMarcella Suelma Torrecillas RosaCamila Albuquerque Mello de CarvalhoDaniela Pretti da Cunha TirapelliFernanda Maris PeriaJosé Joaquim Ribeiro da RochaOmar FéresAbstract Purpose: To evaluate the expression of EGFR, KRAS genes, microRNAs-21 and 203 in colon and rectal cancer samples, correlated with their age at diagnosis, histological subtype, value of pretreatment CEA, TNM staging and clinical outcome. Methods: Expression of genes and microRNAs by real time PCR in tumor and non-tumor samples obtained from surgical treatment of 50 patients. Results: An increased expression of microRNAs-21 and 203 in tumor samples in relation to non-tumor samples was found. There was no statistically significant difference between the expression of these genes and microRNAs when compared to age at diagnosis and histological subtype. The EGFR gene showed higher expression in relation to the value of CEA diagnosis. The expression of microRNA-203 was progressively lower in relation to the TNM staging and was higher in the patient group in clinical remission. Conclusions: The therapy of colon and rectum tumors based on microRNAs remains under investigation reserving huge potential for future applications and clinical interventions in conjunction with existing therapies. We expect, based on the exposed data, to stimulate the development of new therapeutic possibilities, making the treatment of these tumors more effective.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000300243&tlng=enColonic Neoplasms. Rectal Neoplasms. GeneserbB-1. MicroRNAs. |
| spellingShingle | Thais Inácio de Carvalho Paulo Cezar Novais Fermino Sanches Lizarte Neto Renata Danielle Sicchieri Marcella Suelma Torrecillas Rosa Camila Albuquerque Mello de Carvalho Daniela Pretti da Cunha Tirapelli Fernanda Maris Peria José Joaquim Ribeiro da Rocha Omar Féres Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors Acta Cirúrgica Brasileira Colonic Neoplasms. Rectal Neoplasms. Genes erbB-1. MicroRNAs. |
| title | Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors |
| title_full | Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors |
| title_fullStr | Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors |
| title_full_unstemmed | Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors |
| title_short | Analysis of gene expression EGFR and KRAS, microRNA-21 and microRNA-203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors |
| title_sort | analysis of gene expression egfr and kras microrna 21 and microrna 203 in patients with colon and rectal cancer and correlation with clinical outcome and prognostic factors |
| topic | Colonic Neoplasms. Rectal Neoplasms. Genes erbB-1. MicroRNAs. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000300243&tlng=en |
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