cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells
Introduction: Malignant astrocytic gliomas are the most common and lethal brain malignancies due to their refractory to the current therapies. Nowadays, molecular targeted therapy has attracted great attention in treatment of glioma. Connexin 43 (Cx43) and micro ribonucleic acid- 21(miR-21) are amon...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2015-02-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-606-1&slc_lang=en&sid=1 |
| _version_ | 1797284982806282240 |
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| author | Hossein Mostafavi Mojtaba Khaksarian Mohammad Taghi Joghataei Sadegh Yoosefee Mahmoud Reza Hadjighassem Maryam Soleimannejad Raheleh Gholamzadeh Sanam Seifollahi Bahnamiri |
| author_facet | Hossein Mostafavi Mojtaba Khaksarian Mohammad Taghi Joghataei Sadegh Yoosefee Mahmoud Reza Hadjighassem Maryam Soleimannejad Raheleh Gholamzadeh Sanam Seifollahi Bahnamiri |
| author_sort | Hossein Mostafavi |
| collection | DOAJ |
| description | Introduction: Malignant astrocytic gliomas are the most common and lethal brain malignancies
due to their refractory to the current therapies. Nowadays, molecular targeted therapy has
attracted great attention in treatment of glioma. Connexin 43 (Cx43) and micro ribonucleic acid-
21(miR-21) are among molecules that are involved in glioma development and progression.
These molecules showed potential to be as target molecules with regard to glioma. Some studies
have reported that cyclic adenosine monophosphate (cAMP) signaling could be effective on
Cx43 and miR-21 in tissues other than in brain. We investigate possible relationship between
β-adrenergic receptor and its newly described downstream, exchange protein directly activated
by cAMP (Epac) signaling pathway and expression of Cx43 and miR-21 in low (1321N1) and
high grade (U87MG) glioma cell lines. Methods: We treated cells with β-adrenergic agonist and Epac activator with and without adenyl
cyclase inhibitor. Cx43 and miR-21 expression were measured with real-time PCR. Results: Our data showed that in 1321N1 cells, β-adrenergic-Epac pathway stimulation up and
down-regulated Cx43 and miR-21 expression respectively. Whereas, in U87MG cells these
interventions had no effect on Cx43 and miR-21 expression. Discussion: These findings demonstrate that low grade astrocytoma cells have better response to
our pharmacological interventions. |
| first_indexed | 2024-03-07T17:55:38Z |
| format | Article |
| id | doaj.art-382948436496403aa8a9be8f7dc6527a |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:55:38Z |
| publishDate | 2015-02-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-382948436496403aa8a9be8f7dc6527a2024-03-02T12:14:10ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422015-02-01615257cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma CellsHossein Mostafavi0Mojtaba Khaksarian1Mohammad Taghi Joghataei2Sadegh Yoosefee3Mahmoud Reza Hadjighassem4Maryam Soleimannejad5Raheleh Gholamzadeh6Sanam Seifollahi Bahnamiri7 Department of Physiology and Pharmacology, Zanjan University of Medical Sciences Department of Physiology, Lorestan University of Medical Sciences Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences Introduction: Malignant astrocytic gliomas are the most common and lethal brain malignancies due to their refractory to the current therapies. Nowadays, molecular targeted therapy has attracted great attention in treatment of glioma. Connexin 43 (Cx43) and micro ribonucleic acid- 21(miR-21) are among molecules that are involved in glioma development and progression. These molecules showed potential to be as target molecules with regard to glioma. Some studies have reported that cyclic adenosine monophosphate (cAMP) signaling could be effective on Cx43 and miR-21 in tissues other than in brain. We investigate possible relationship between β-adrenergic receptor and its newly described downstream, exchange protein directly activated by cAMP (Epac) signaling pathway and expression of Cx43 and miR-21 in low (1321N1) and high grade (U87MG) glioma cell lines. Methods: We treated cells with β-adrenergic agonist and Epac activator with and without adenyl cyclase inhibitor. Cx43 and miR-21 expression were measured with real-time PCR. Results: Our data showed that in 1321N1 cells, β-adrenergic-Epac pathway stimulation up and down-regulated Cx43 and miR-21 expression respectively. Whereas, in U87MG cells these interventions had no effect on Cx43 and miR-21 expression. Discussion: These findings demonstrate that low grade astrocytoma cells have better response to our pharmacological interventions.http://bcn.iums.ac.ir/browse.php?a_code=A-10-606-1&slc_lang=en&sid=1cAMP Epac Cx43 miR- 21 glioma 1321N1 U87MG beta adrenergic receptor |
| spellingShingle | Hossein Mostafavi Mojtaba Khaksarian Mohammad Taghi Joghataei Sadegh Yoosefee Mahmoud Reza Hadjighassem Maryam Soleimannejad Raheleh Gholamzadeh Sanam Seifollahi Bahnamiri cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells Basic and Clinical Neuroscience cAMP Epac Cx43 miR- 21 glioma 1321N1 U87MG beta adrenergic receptor |
| title | cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells |
| title_full | cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells |
| title_fullStr | cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells |
| title_full_unstemmed | cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells |
| title_short | cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells |
| title_sort | camp epac pathway stimulation modulate connexin 43 and microrna 21 expression in glioma cells |
| topic | cAMP Epac Cx43 miR- 21 glioma 1321N1 U87MG beta adrenergic receptor |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-606-1&slc_lang=en&sid=1 |
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