Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
Over the last several decades, kiwifruit production has been severely damaged by the bacterial plant pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa), resulting in severe economic losses worldwide. Currently, copper bactericides and antibiotics are the main to...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/12/2704 |
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| author | Jinyan Luo Dejiang Dai Luqiong Lv Temoor Ahmed Lei Chen Yanli Wang Qianli An Guochang Sun Bin Li |
| author_facet | Jinyan Luo Dejiang Dai Luqiong Lv Temoor Ahmed Lei Chen Yanli Wang Qianli An Guochang Sun Bin Li |
| author_sort | Jinyan Luo |
| collection | DOAJ |
| description | Over the last several decades, kiwifruit production has been severely damaged by the bacterial plant pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa), resulting in severe economic losses worldwide. Currently, copper bactericides and antibiotics are the main tools used to control this bacterial disease. However, their use is becoming increasingly ineffective due to the emergence of antibiotic resistance. In addition, environmental issues and the changes in the composition of soil bacterial communities are also concerning when using these substances. Although biocontrol methods have shown promising antibacterial effects on Psa infection under in vitro conditions, the efficiency of antagonistic bacteria and fungi when deployed under field conditions remains unclear. Therefore, it is crucial to develop a phage-based biocontrol strategy for this bacterial pathogen. Due to the specificity of the target bacteria and for the benefit of the environment, bacteriophages (phages) have been widely regarded as promising biological agents to control plant, animal, and human bacterial diseases. An increasing number of studies focus on the use of phages for the control of plant diseases, including the kiwifruit bacterial canker. In this review, we first introduce the characteristics of the Psa-induced kiwifruit canker, followed by a description of the diversity and virulence of Psa strains. The main focus of the review is the description of recent advances in the isolation of Psa phages and their characterization, including morphology, host range, lytic activity, genome characterization, and lysis mechanism, but we also describe the biocontrol strategies together with potential challenges introduced by abiotic factors, such as high temperature, extreme pH, and UV irradiation in kiwifruit orchards. The information presented in this review highlights the potential role of phages in controlling Psa infection to ensure plant protection. |
| first_indexed | 2024-03-09T15:44:34Z |
| format | Article |
| id | doaj.art-382b6cb0f59e4216b13b09d070dc1698 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T15:44:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-382b6cb0f59e4216b13b09d070dc16982023-11-24T18:38:07ZengMDPI AGViruses1999-49152022-12-011412270410.3390/v14122704Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>Jinyan Luo0Dejiang Dai1Luqiong Lv2Temoor Ahmed3Lei Chen4Yanli Wang5Qianli An6Guochang Sun7Bin Li8Department of Plant Quarantine, Shanghai Extension and Service Center of Agriculture Technology, Shanghai 201103, ChinaStation for the Plant Protection & Quarantine and Control of Agrochemicals Zhejiang Province, Hangzhou 310004, ChinaState Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Plant Quarantine, Shanghai Extension and Service Center of Agriculture Technology, Shanghai 201103, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaOver the last several decades, kiwifruit production has been severely damaged by the bacterial plant pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa), resulting in severe economic losses worldwide. Currently, copper bactericides and antibiotics are the main tools used to control this bacterial disease. However, their use is becoming increasingly ineffective due to the emergence of antibiotic resistance. In addition, environmental issues and the changes in the composition of soil bacterial communities are also concerning when using these substances. Although biocontrol methods have shown promising antibacterial effects on Psa infection under in vitro conditions, the efficiency of antagonistic bacteria and fungi when deployed under field conditions remains unclear. Therefore, it is crucial to develop a phage-based biocontrol strategy for this bacterial pathogen. Due to the specificity of the target bacteria and for the benefit of the environment, bacteriophages (phages) have been widely regarded as promising biological agents to control plant, animal, and human bacterial diseases. An increasing number of studies focus on the use of phages for the control of plant diseases, including the kiwifruit bacterial canker. In this review, we first introduce the characteristics of the Psa-induced kiwifruit canker, followed by a description of the diversity and virulence of Psa strains. The main focus of the review is the description of recent advances in the isolation of Psa phages and their characterization, including morphology, host range, lytic activity, genome characterization, and lysis mechanism, but we also describe the biocontrol strategies together with potential challenges introduced by abiotic factors, such as high temperature, extreme pH, and UV irradiation in kiwifruit orchards. The information presented in this review highlights the potential role of phages in controlling Psa infection to ensure plant protection.https://www.mdpi.com/1999-4915/14/12/2704infectiondiversitygenomekiwifruit cankerphage-based control |
| spellingShingle | Jinyan Luo Dejiang Dai Luqiong Lv Temoor Ahmed Lei Chen Yanli Wang Qianli An Guochang Sun Bin Li Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> Viruses infection diversity genome kiwifruit canker phage-based control |
| title | Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_full | Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_fullStr | Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_full_unstemmed | Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_short | Advancements in the Use of Bacteriophages to Combat the Kiwifruit Canker Phytopathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_sort | advancements in the use of bacteriophages to combat the kiwifruit canker phytopathogen i pseudomonas syringae i pv i actinidiae i |
| topic | infection diversity genome kiwifruit canker phage-based control |
| url | https://www.mdpi.com/1999-4915/14/12/2704 |
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